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HAMINI KADAR Nisserine
Microbiologie Appliquée - LMA
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Article 08 :Année de publication 2015
Titre An in vitro antagonistic activity evaluation of rhizobacteria against Fusarium oxysporum f. sp. radicis-lycopersici (Forl) isolated from the Algerian west.
   
Revue, référence International Journal of Biosciences, ISSN 2222-5234
   
Autres auteurs Wassim Yezli
Nebia Zebboudj
Nisserine Hamini
Kadar
Mebrouk Kihal
Henni Jamal Eddine
   
Mots Clefs Fusarium oxysporum f. sp. radicis-lycopersici, Biological control, Bacillus subtilis, Pseudomonas fluorescens, Leuconostoc spp, Fusarium crown, Root rot.
   
Résumé
   The use of biological antagonists is a promising technique for the protection of plants without having a fatal effect on the environment. The in vitro confrontation tests is an obligatory step for the selection of successful antagonistic agents, what has ends in our study to the selection of two rhizobacteria: Bacillus subtilis and Pseudomonas fluorescens, isolated from the rhizosphere of tomato healthy plants and which were tested for their in vitro inhibitive power against five isolates of Fusarium oxysporum f. sp. radicis-lycopersici (Forl). The results of the direct confrontation on different culture media demonstrated that rhizobacteria have an inhibitive effect on the pathogenic, while noticing a performance of P. fluorescens with an inhibition which varies between 73,77 and 80 %, comparing with 56,36 and 60,46 % for B. subtilis. The inhibition by volatile substances of P. fluorescens varies between 25 and 39,53 %, compared with 18,86 and 32,55 % by volatile substances of B. subtilis. Rhizobacterial cell-free supernatant showed an inhibition from the first until the seventh incubation day against the five fungal isolates, when mycelia growth reaches 5±0,2 cm in presence of P. fluorescens cell free supernatant, and it reaches 5,9±0,3 cm in presence of B. subtilis cell free supernatant compared with 8,5 cm on control plates without rhizobacteria cell free supernatant.
Article 07 :Année de publication 2014
Titre Antifungal activity of lactic acid bacteria against Fusarium oxysporum f. sp. albedinis isolated from diseased date palm in South Algeria
   
Revue, référence International Journal of Biosciences, ISSN 2222-5234
   
Autres auteurs Nebia Zebboudj
Wassim Yezli
Nisserine Hamini
Kadar
Mebrouk Kihal
Henni Jamal Eddine
   
Mots Clefs Fusarium oxysporum f. sp. albedinis, Antifungal activity, Lactic acid bacteria, Lactococcus spp, Leuconostoc spp
   
Résumé
   In this study, four lactic acid bacteria (Lactococcus lactissubsp. lactis, Lactococcus lactis subsp. lactisbiovar. diacetylactis, Leuconostoc mesenteroidessubsp. mesenteroides and Leuconostoc mesenteroidessubsp. Mesenteroides biovar. Dextranicum ) were screened in vitro for antifungal activity against twelve strains of Fusarium oxysporum f. sp. albedinis (Foa), causal agent of the “Bayoud”, the most destructive disease of date palm (Phoenix dactylifera L.) in Algeria. The results of the direct confrontation of LAB against (Foa) on MRS agar and PDA medium showed that there is a better inhibition by the LAB on MRS agar. Lactococcus lactis subsp.lactis biovar. Diacetylactis gave the most effective rate inhibition, between 13.51 an d 40.29 % on PDA medium and 41.17 and 100% on MRS Agar medium, followed by the other strains with inhibition reached 35.82 % on PDA medium and between 19.29 and 100 % on MRS agar medium. When we used cell-free supernatant, there was appearance of inhibition zones after 24 h incubation, with a percentage of inhibition reached 49.41 %. The microscopic observations in the areas of confrontation of the fungus with the LAB have shown that there is an inhibition of the conidia germination.
Article 06 :Année de publication 2014
Titre Thermophilic Fungi Causing Deterioration of Amylase Activities of Ascochyta Pisi Causing Anthracnose of Peas (Pisum sativum).
   
Revue, référence Advances in Environmental Biology, ISSN 1998-1066/1995-0756
   
Autres auteurs Yakoubi Meryem
Benichou Soumaya Lalia
Terbeche Rym
Hamini
Kadar Nisserine
Kihal Mebrouk
Henni Jamal Eddine
   
Mots Clefs
   
Résumé
    The anthracnose of pea caused by the phytopathogenic fungus Ascochyta pisi Lib.is one of The fungal diseases transmissible by the seeds causing necrotic spots on the different organs of pea plants (Pisum sativum L.), and making them improper for commercialization. Indeed, this parasite is carried out of very powerful enzymatic equipment which plays a role in its pathogenecity among which the amylase.The determination of the amylolytic activity in the solid medium (Czapek modified with different concentrations of starch), can confirm the presence of an enzyme complex necessary for the hydrolysis of starch present in the medium and shows that the optimum growth for the three isolates of A.pisi were obtained from a higher concentration of starch (Czapek + 2% of starch). The study of the amylolytic activity of the three isolates on medium Czapek + 2% of starch with different pH (4, 6, 8) and at various times from incubation, shows that the optimum for the amylolytic activity for all of our isolates occurs at pH values between 6 and 7 and away from either side of these limits the amylolytic activity declines.
Article 05 :Année de publication 2014
Titre ANTIFUNGAL ACTIVITY OF CLOVE (Syzygium aromaticum L.) ESSENTIAL OIL AGAINST PHYTOPATHOGENIC FUNGI OF TOMATO (Solanum lycopersicum L) IN ALGERIA
   
Revue, référence Journal of Experimental Biology and Agricultural Sciences, ISSN No. 2320 – 8694
   
Autres auteurs Hamini
Kadar Nisserine
Hamdane Fatima
Boutoutaou Rachida
Kihal Mebrouk
Henni Jamal Eddine
   
Mots Clefs Antifungal Activity, Essential Oil, Syzygium aromaticum, Fusarium oxysporum f.sp radicis lycopercis,Fusarium commune, Fusarium redolens
   
Résumé
    Present study was conducted to find the efficiency of Syzygium aromaticum [L.] essential oil in management of three pathogenic fungus viz Fusarium oxysporum f.sp radicis lycopercisi, F. commune and F. redolens. The yield of hydro-distilled essential oil was also computed (7.49%) in study. The results of study revealed moderate to high level antifungal activities against tested microorganisms at all the tested concentration. The highest antifungal activity was recorded at 1μLml-1; on this concentration clove essential oil caused complete growth inhibition of F. oxysporum f.sp radicis lycopercisi and F. redolens while the highest antifungal activity of oil against F. commune was observed at 0.5 μLml-1.
Article 04 :Année de publication 2014
Titre In vitro effect of two fungicides on pathogenic fungi causing root rot on tomato in Algeria.
   
Revue, référence African Journal of Agricultural Research, ISSN: 1991-637X DOI: 10.5897/AJAR
   
Autres auteurs Nisserine Hamini
Kadar
Hadjira Benaouili
Soumaya Benichou
Mebrouk Kihal
Setti Benali
Henni Jamal Eddine
   
Mots Clefs Fusarium species, mycelium growth, inhibition, Trifidan, Antracol, tomato diseases, control, fungicides
   
Résumé
   Tomato (Lycopersiconesculentum) is one of the mostwidely grown vegetables in the world. There is a growing concern in recent years, both in developed anddeveloping countries, about the use of hazardous fungicides for controllingplant diseases.Thein vitroeffectof two fungicides commonly used in Algeria namely Trifidan andAntracol (propineb)on the growth of three pathogenic fungi of tomato, Fusariumoxysporumf.sp. radicis-lycopersici, F. commune and Fusariumredolenswas investigated to determine the effectiveness of the fungicides in reducing fungi growth. Each fungicide was assayed at 0, 100, 200, 400 and 500 mg L-1 rate in Potato Dextrose Broth (PDB) and incubated at 28°C for seven days.Data were collected on the mycelia weights of the fungi under each treatment.Mycelia weights of the three fungi were significantly reduced at 100 mg L-1 by the two fungicides under antracol treatment Fusariumredolens and Fusarium communeand asignificant reduction was observed at 400 and 500 mgL-1. Application of Trifidan,significantly decrease in mycelia weight of F. oxysporum f. sp. radicis-lycopersici and F. communeirrespective of the rate applied. F. redolenswas completely inhibited by Trifidan, and its inhibition started at100 mgL-1.
Article 03 :Année de publication 2014
Titre Isolation, pathogenicity test and physicochemical studies of Fusarium oxysporum f.sp radicis lycopersici
   
Revue, référence Advances in Environmental Biology, ISSN 1998-1066/1995-0756
   
Autres auteurs Benaouili Hadjira
Hamini
Kadar Nisserine
Bouras Aicha
Soumaya Benichou
Mebrouk Kihal
Henni Jamal Eddine
   
Mots Clefs Fusarium oxysporum, pathogenicity test, various media, pH, temperature, light humidity, Carbone, Nitrogen.
   
Résumé
   The study of 27 isolates of F.oxysporum from different regions of western Algeria(Oran, Mostaganem, Chleff) Allowed on the basis of their macroscopic appearance to differentiate four morphotypes (downy, cottony, ras mucous and senescent) with different pigmentation (white, yellow, pink and purple).The pathogenicity test confirmed the presence of Fusarium oxysporum f.sp radicis lycopersici in the western region of Algeria. Of the 27 strains studied 22 have shown signs of crown and root rot, with very high degrees of virulence compared to the references strains and 5 isolates were not pathogen. For a better understanding of the ecology of these isolates a study of the influence of different physicochemical conditions were realized. The results showed a better growth of the isolates on PDA medium with an important pigmentation, mycelia growth of different isolates is quiet faster in the darkness than in light and the optimum pH for growth was 6 and 7 with a rate of humidity ranging from 74 to 80% and an optimal growth temperature of 23°C and 28°C.
Article 02 :Année de publication 2014
Titre Antagonist effect of nonpathogenic Fusarium oxysporum strain Fo47 on Fusarium oxysporum f. sp. radicis-lycopersici the cause of fusarium crown and root rot in tomato cultivation
   
Revue, référence Advances in Environmental Biology, ISSN 1998-1066/1995-0756
   
Autres auteurs Bouras Aicha
Hamini
Kadar Nisserine
Si Mohamed Abdeslem
Benaouili Hadjira
Soumaya Benichou
Mebrouk Kihal
Henni Jamal Eddine
   
Mots Clefs Fusarium oxysporum,f.sp radicis lycopersici, tomato, root rot and crown, disease incidence, biocontrol, nonpathogenic Fo47.
   
Résumé
   Root rot and crown rot caused by Fusarium oxysporum f.sp. radicis-lycopersici (Forl) is one of the most serious and complex wilt root rot diseases affecting crops of tomato (Solanum lycopersicum L.) over the world. In this study, the antagonist effect of nonpathogenic Fusarium oxysporum strain Fo47 on the pathogen Forl was evaluated in vivo, in growth chamber by using two bioessays, in which, the investigated mechanisms involved the direct antagonism, including a competition for nutriments and root colonization, using a temporal separation of the antagonist ant the pathogen on the one hand and inducing systemic resistance to the tomato plant, using a physically separation of the antagonist and the pathogen to the same plant root level with a split-root method on the other. Results obtained from the two bioassays showed that the nonpathogenic Fo47 have significantly reduced the incidence of the disease while being separated in time or space of the pathogenic strain, especially when they were applied a week before it. These results have important practical implications for biocontrol of tomato diseases under commercial conditions.
Article 01 :Année de publication 2010
Titre First report of Fusarium commune and Fusarium redolens causing crown and root rot on tomato in Algeria.
   
Revue, référence New disease reports, New Disease Reports 22, 3.
   
Autres auteurs Hamini
Kadar N Edel
Hermann V Gautheron
N Steinberg C
   
Mots Clefs tomato, Fusarium oxysporum, Fusarium redolens, Fusarium commune
   
Résumé
   Tomato (Solanum lycopersicum) is a common crop in Algeria. From 2006 to 2008, surveys were conducted in the tomato-growing areas of Algeria around Alger and Oran where diseased plants were found with yellow discolouration of the vascular tissue in stems, wilting of the lower leaves and crown and root rot. Fungal isolates with morphological characteristics similar to those of Fusarium oxysporum (Nelson et al., 1983) were collected from diseased plants and preserved in the MIAE collection (Microorganisms of Interest for Agriculture and Environment, INRA Dijon, France). Four isolates were identified using a two-locus molecular strategy recommended for Fusarium species, based on sequences of the internal transcribed spacer (ITS) region and the elongation factor EF-1a gene (Geiser et al., 2004). GenBank Accession Nos. are HM584894 to HM584901. A BLAST search identifiedisolates MIAE00129 and MIAE00131 as F. redolens and isolates MIAE00101 and MIAE00112 as F. commune based on 100% similarity with corresponding sequences. F. commune is a recently described species closely related to, but phylogenetically independent of F. oxysporum (Skovgaard et al., 2003). The strains were evaluated for their pathogenicity on tomato. For each strain, ten 12-day-old seedlings of tomato cv. Monfavet 63.5 were inoculated by cutting the roots 7 mm below the crown and dipping the plants for five minutes in 5 ml of a suspension of 10 conidia/ml in minimal liquid medium (MLM). Plants were transplanted into perlite and the conidial suspension was poured onto the perlite around the crown. Ten non-inoculated plants were prepared using MLM instead of conidial suspension. Plants were incubated in a climatic chamber at 24°C during the day (16 h) and 20°C at night for four weeks. The percentages of plants affected (dead plants together with plants showing dark brown lesions on crown and roots) were 40% and 50% for F. redolens MIAE00129 and MIAE00131 respectively, and 80% and 0% for F. commune MIAE00101 and MIAE00112 respectively. No symptoms were observed on non-inoculated plants. The pathogenicity tests were performed again at a temperature of 28°C instead of 24°C during the day. The percentages of plants affected were 40% for MIAE00101 but 100% for the three other strains. To our knowledge, this is the first report of F. commune and F. redolens being pathogenic on tomato in Algeria.



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