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HENNI Jamal eddine
Microbiologie Appliquée - LMA
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Article 38 :Année de publication 2013
Titre Carbon sources and pH effect on pectinolytic activity production by Ascochyta rabiei isolated from chickpea (Cicer arietinum L.) in West Algeria
   
Revue, référence African Journal of Microbiology Research, Vol. 7(27), pp. 3483-3488
   
Autres auteurs Gharbi S
Karkachi N
Kihal M
Henni J.
   
Mots Clefs Ascochyta rabiei; pectinolytic activities; pectin méthylesterase; polygalacturonase
   
Résumé
   In Algeria, Ascochyta blight is a major limiting factor in chickpea production. We did not record any difference between the isolates while basing ourselves on the morphological and cultural characters, but the pectinolytic activity could differentiate the ten isolates of Ascochyta rabiei. Activity of pectin methyl esterase was baseless in the presence of glucose, but variable in the presence of the citrus pectin and polygalacturonic acid. We recorded the weak production ranging between 0.19 and 0.55 µeg/ml/min; however the strong activity was between 2.97 and 5.88 µeg/ml/min in the presence of citrus pectin and polygalacturonic acid, respectively. On the other hand, the polygalacturonase activity was present in culture filtrates using three sources of carbon. Weak production was recorded in the presence of glucose (0.28 to 2.63 µmole/min), however the strong activity was recorded in the presence of 16.29 µmole/min of polygalacturonic acid. The maximal production of polygalacturonase was 11.7 µmole/min in the presence of the polygalacturonic acid and 0.9 µmole/min in the presence of glucose after six days of incubation at pH 5. The polygalacturonase activity was between 0.3 and 0.62 µmole/min after 4 days of incubation at pH 8. However, optimum production was obtained at pH 5.5. It should be noted that there is an important difference in the production of polygalacturonase with simple sugars glucose and pectin, indicating that the production of the enzyme is also stimulated by the presence of inducers. The exo-polygalacturonase is present in all carbon sources, suggesting that this enzyme could probably be of a constitutive nature in Ascochyta rabiei.
Article 37 :Année de publication 2013
Titre Physico-chimical and microbiological analysis of algerien raw camel’s milk and identification of predominating thermophylic lactic acid bacteria
   
Revue, référence Journal of food science and engineering, 3 (2013) 55-63
   
Autres auteurs Merzoug Yamina
Chahrour Wassila
Zarour Kenza
Zergui Amina
Saidi Noureddine
Henni J
Kihal M.
   
Mots Clefs Raw camel’s milk; physicochemical analysis; microflora; electrophoretic analysis; proteins of milk
   
Résumé
   The dromedary camel (Camelus dromedarius) is a significant socioeconomic importance in several arid and semi-arid regions of North Africa and Middle East, and its milk constitutes an important component of human diets in these regions. The camel milk plays a vital role in the food of the Algerian nomads in the Sahara. During February and September, 20 samples of the raw camel’s milk were taken starting from different livestock of camels from three different Sahariennes regions (Bechar, El-Bayadh and Naama). These 20 collected samples were analyzed by physico-chemical and microbiological methods. The results of physicochemical analyze obtained from two hot and cold seasons are respectively the following: T °C (35.83 and 33.95), pH (6.36 and 6.49), density (1.031 and 1.032), dornic acidity (18.6 and 18.3 °D), dry matter (93.4 and 144.8 g/L), fat contents (30 and 52.1 g/L), total protein (26.3 and 33.1 g/L) and ashes (7.46 and 8.66 g/L). The protein profile obtained by electrophoretic analysis (SDS-PAGE) showed that camel milk contains several types of proteins and some have a molecular weight identical to major proteins of the cow’s milk. The final results showed that camel milk has generally a comparable composition to that of bovine milk. The microbiological analysis, of these samples, detected a significant number of the total microflora, Staphylococcus aureus and total coliforms. The absence of Clostridium and fecal coliforms was observed. Several species of lactic acid bacteria were detected such as Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis biovar. diacetylactis, Weissella cibaria and Enteroccocus feacalis.
Article 36 :Année de publication 2013
Titre Screening and identification of lactic acid bacteria isolated from sorghum silage processes in west Algeria
   
Revue, référence African Journal of Biotechnology , Vol. 12(14), pp. 1703-1709
   
Autres auteurs Chahrour W
Merzouk Y
Henni J. E
Haddaji M
Kihal M.
   
Mots Clefs Lactic acid bacteria; identification; silage; sorghum; evolution; amylolytic; technology; species
   
Résumé
   The lactic acid bacteria (LAB) isolated from sorghum (Sorghum bicolor. L.) silage were identified during different periods of evolution of sorghum silage in west Algeria. Morphological, physiological, biochemical and technological techniques were used to characterize lactic acid bacteria isolates. A total number of 27 representatives of lactic acid bacterial strains were retained and among them four dominant genus were identified as Lactobacillus (44%), Lactococcus (14.81%), Weissella (29.62%) and Leuconostoc (11.11%). The representative species identified were Lactobacillus brevis (25%), Lactobacillus pentosus (3.7%), Lactobacillus manihotivorans (11.11%), and Lactobacillus fermentum (3.7%). Lactococcus lactis subsp. lactis biovar. diacetylactis (14.81%), Weissella cibaria (7.2%), Weissella minor (11.11%), Weissella soli (3.7%), Weissella viridescense (7.2%) and Leuconostoc mesenteroides subsp. mesenteroides (11.11%). Only two strains of lactic acid bacteria were amylolytic. These results will enable future research on the relationship between LAB species and silage fermentation quality.
Article 35 :Année de publication 2012
Titre Screening of autochthonous Lactobacillus species from Algerian raw goats’ milk for the production of bacteriocin-like compounds against Staphylococcus aureus
   
Revue, référence African Journal of Microbiology Research, African Journal of Microbiology Research Vol. 6(12), pp. 2888-2898, 30 March, 2012
   
Autres auteurs Mami Anas
Boumehira Ali Zinedine
Hamedi Amine Rizk
Henni Jamal Eddine
Kihal Mebrouk
   
Mots Clefs Raw goats’ milk, lactic acid bacteria, Lactobacillus plantarum, interaction, bacteriocin, Staphylococcus, mixed, culture.
   
Résumé
   Lactic acid bacteria play a key role in maintaining the balance of normal gastro-intestinal micro flora. Food contamination by Staphylococcus aureus is a major problem for consumer’s health in Algeria, especially during the summer period. The use of bacterial interactions is a new way to limit the pathogenic germs growth. Detection of antimicrobial substances produced by lactic acid bacteria against the undesirable germs is the objective of this work. Microbiological and biochemical methods were used to identify lactic acid bacteria having an antimicrobial activity. The 2 isolates of lactic acid bacteria obtained from raw goats’ milk in western Algeria's areas were identified: Lactobacillus rhamnosus, Lactobacillus plantarum. The interactions study revealed that three lactobacilli species: L. plantarum (58) and L. plantarum (68) are able to inhibit S. aureus’ growth. In mixed culture after 24 h, L. plantarum reduces the growth of S. aureus by 1.6 log and this latter bacteria was not found after 72 h. The various tests used reveal the proteinic nature of the substance which was responsible for the growth inhibition of S. aureus. The ecological adaptation and growth characteristics of cultures of L. plantarum in food products will determine their effectiveness as bio-control agent in dairy food
Article 34 :Année de publication 2012
Titre Bioprospecting of Leuconostoc mesenteroides strains isolated from Algerian raw camel and goat milk for technological properties useful as adjunct starters
   
Revue, référence African Journal of Microbiology Research, African Journal of Microbiology Research Vol. 6(13), pp. 3192-3201, 9 April,
   
Autres auteurs Zarour K.
Benmechernene Z.
Hadadji M
Moussa
Boudjemaa B
Henni D. J.
Kihal M
   
Mots Clefs Technological, yeast extract, Leuconostoc, milk, antibiotics, growth kinetic, carbon dioxide
   
Résumé
   Leuconostoc species are lactic acid bacteria widely used in milk fermentation. Based on morphological, physiological and biochemical analysis, 18 strains of Leuconostoc mesenteroides were isolated and identified from 10 samples of goat's milk and camel's milk. Strains were identified as follows 09 strains of L. mesenteroides subsp. mesenteroides and 09 strains of L. mesenteroides subsp. dextranicum. The results of technological tests of the strains showed that strains produced dextran, carbon dioxide, and resist to 55°C for 15 min, which promote their industrial use. The growth kinetic, acidification evolution and carbon dioxide production of L. mesenteroides subsp. dextranicum strain in skim milk at 30°C were slightly higher than L. mesenteroides subsp. mesenteroides. Addition of yeast extract to skim milk stimulates the development of L. mesenteroides subsp. mesenteroides by increasing its growth, acidification activity and evolved CO2. Susceptibility to antibiotics was also evaluated on 33 antibiotics and strains of L. mesenteroides showed resistance to 42.4% of antibiotics used.
Article 33 :Année de publication 2012
Titre Caractérisation microbiologique et technologique des espèces de Leuconostoc mesenteroïdes isolées du lait cru de chèvre et de chamelle d’Algérie
   
Revue, référence Nature & Technology, Nature et Technology
   
Autres auteurs Zarour Kenza
Henni Jamal Eddine
Kihal Mebrouk
   
Mots Clefs Technological milk, Leuconostoc, growth kinetics, carbon dioxide, antibiotics, yeast extract
   
Résumé
   La caractérisation microbiologique et technologique de l’espèce Leuconostoc mesenteroïdes isolée du lait cru de chèvre, de chamelle et du fromage du Roquefort ont permis d’identifier neuf souches de Ln. mesenteroïdes subsp. mesenteroïdes et neuf souches de Ln. mesenteroïdes subsp. dextranicum. Les résultats des tests technologiques ont montré que les isolats produisent le dextrane sur milieu MSE, et le dioxyde carbone, utilisent le citrate et résistent à 55°C pendant 15 minutes, ce qui favorisent leur utilisation industrielle. La souche de Ln. mesenteroïdes subsp. dextranicum a une cinétique de croissance, d’acidification et de production de dioxyde de carbone dans le lait écrémé à 30°C, un peu plus élevée que Ln. mesenteroïdes subsp. mesenteroïdes . L’addition de l’extrait de levure au lait écrémé stimule le développement de Ln. mesenteroïdes subsp. mesenteroïdes en augmentant sa croissance, son activité acidifiante et gazogène. La sensibilité des souches aux antibiotiques a été évaluée sur 33 antibiotiques et les souches ont montré une résistance à 42,4% des antibiotiques utilisés.
Article 32 :Année de publication 2012
Titre Microsatellites Markers, an Efficient Tool in Phytopathogenic Fungus Population Studies: Background and Applications.
   
Revue, référence European Journal of Scientific Research, Vol. 84 No.2 (2012), pp.223 – 235
   
Autres auteurs Setti Benali
M. Bencheikh
Jamal Eddine HENNI
   
Mots Clefs Microsatellites, SSR, population genetics, plant pathogen, fungi
   
Résumé
   During the last decade, advances in molecular biology have led to the emergence of microsatellites as powerful molecular markers for the study of the plant pathogen populations. Microsatellites or short tandem repeats of DNA sequence motifs of 1 to 6 bases pairs in the length, which are highly abundant, hypervariable, codominant and multiallelic. They are also distributed throughout the eukaryotic genome and are detected by polymerase chain reaction. These features make them a marker of choice of numerous applications including genetic diversity, population structure, strain identification and fingerprinting. But the major drawback of microsatellites remains their isolation and their development which is extremely expensive and time consuming. Meanwhile, the reducing cost of genome sequencing technology has made an increasing numbers of publicly microsatellites databases which can be freely mined using suitable search software. The purpose of this review is to present the most relevant published reports with emphasis on the use of the microsatellites markers for the population pathogen genetics. Among the aspects reviewed in this paper includes the distribution and mutations of microsatellites, the main method used for isolation and development of SSR loci and the in silico mining of microsatellites through the publicly databases. Thereafter, we present the various applications of microsatellites in molecular plant pathology and particularly in fungal populations’ studies.
Article 31 :Année de publication 2012
Titre Genetic variability and population structure of Mycosphaerella pinodes in western Algeria using AFLP fingerprinting.
   
Revue, référence Journal of Plant Pathology, 94 (1), 127-133.
   
Autres auteurs Setti Benali
Jamal Eddine HENNI
C. Neema.
   
Mots Clefs AFLP, genetic diversity, Mycosphaerella pinodes, Pisum sativum, population structure.
   
Résumé
   The genetic diversity and population structure of Mycosphaerella pinodes (Dydimella pinodes) in Algeria were investigated by AFLP markers. A total of 75 isolates from different collection sites corresponding to four geographic regions were analyzed. Data from AFLP loci were used to estimate gene diversity, genetic distances, and to make indirect measures of gene flow between population groups. Extensive diversity was detected in the M. pinodes population regardless of the population group. The percentage of polymorphic loci varied from 41% in the semi-arid superior region to 50% in the sub-humid region. Nei’s gene diversity across loci was 0.471 and the Shannon’s index across loci was 0.663. A high level of differentiation (Gst = 0.308) and a low gene flow (Nm = 1.118) among population groups were found. Based on the analysis of molecular variance, 58% of the genetic variation of M. pinodes was within populations, 31% of the variation was between geographical regions and 11% among populations within a region. Cluster analyses using individual isolates failed to group them according to population. This is the first report on genetic diversity and population structure of M. pinodes on pea in Algeria.
Article 30 :Année de publication 2011
Titre Prevalence of mastitis infection in cattle in West Algeria and Identification of causing bacteria
   
Revue, référence Journal of Animal and Veterinary Advances, 10.22-3002-3005
   
Autres auteurs N. BENHAMED
M. MOULAY
H. AGGAD
J. E. HENNI
M. KIHAL
   
Mots Clefs Mastitis, Streptococcus, Staphylococcus, Enterobacteria, cow health, quality, hygiene, raw milk., Mastitis, Streptococcus, Staphylococcus, Enterobacteria, cow
   
Résumé
   Mastitis is a common disease that decreases milk yield and quality in lactating cows. Because of higher treatment cost and higher culling rate, mastitis causes economical losses. The CMT test was used to detect infection of mastitis in the farms; the infection is detected in 55.16% of cattle herd. The responsible infections bacteria suspected mastitis were determined .The bacteriological analysis revealed the presence of varied dominant bacteria belonging to the species of Staphylococcus aureus (30.76%), Streptococcus sp. (30.76%) and Escherichia coli (23.07%). Theses bacterial species were responsible for the infection of the cow in the different samples .The milk acidity was variable, so that almost half of the samples were at pH (6.65 to 6.90). The establishment of a guide to monitoring and surveillance is essential for control of pathogenic bacteria.
Article 29 :Année de publication 2011
Titre Applications of response surface methodology approach to determine the effect of temperature, time of incubation and light conditions on germination and germ tube growth of Puccinia coronata f.sp. avenae urediosopores
   
Revue, référence African Journal of Biotechnology, Vol. 10(46), pp. 9421-9427
   
Autres auteurs Benali Setti
Mohamed Bencheikh
Jamel Eddine Henni
   
Mots Clefs Puccinia coronata f.sp. avenae, temperature, time, illuminations, conditions, germination process, germ tube growth, urediospores.
   
Résumé
   Crown rust caused by Puccinia coronata f.sp. avenae is the most damaging disease on oat. This work analyzed the effects of temperature and illumination regime during different time of incubation on both spore germination and germ tube growth, using both analysis of variance (ANOVA) and response surface methodology (RSM). This study reveals that the maximum of germination approached 95% under dark conditions at 20°C. Similarly, the maximum germ tube length was 125±23 μm under dark conditions at the same temperature after 18 h. Both spore germination and germ tube growth were observed over a wider temperature range of 5 to 30°C. The darkness conditions seem to enhance significantly (P < 0.05) both the germination and germ tube growth. After 4 h of incubation, germination was significantly higher under darkness regime at 15, 20 and 25°C than under light conditions. The effect of darkness conditions on germ tube growth paralleled its effect on germination. Furthermore, the response surface methodology (RSM) was applied to determine the optimal conditions of temperature, time and illuminations conditions for both the germination process and germ tube growth of urediospores for P. coronata f.sp. coronata. Values for the optimal germination and germ tube growth were 20°C and 8 h under darkness conditions. Moreover, urediospores of P. coronata f.sp. coronata germination and germ tube growth had followed a quadratic response function on temperature (R² = 0.94 and 0.97). On the other hand, the experimental values were in good agreement with the predicted ones and the model was highly significant with the correlation coefficient R being 0.97 and 0.98, respectively for germination and germ tube growth.
Article 28 :Année de publication 2011
Titre Morphological and virulence variation among isolates of Mycosphaerella pinodes the causal agent of pea leaf blight
   
Revue, référence African Journal of Agricultural Research, Vol. 6(5), pp. 1067-1075
   
Autres auteurs Benali Setti
Mohamed Bencheikh
Jamel Henni
Claire Neema
   
Mots Clefs Mycosphaerella blight, morphometry, cluster analysis
   
Résumé
   Mycosphaerella blight caused by Mycosphaerella pinodes (Berk. and Blox.) Vestergr. is an important disease, causing severe damage in peas. Variability of 20 Algerian isolates of M. pinodes representative of four agro climatic regions were investigated on the basis of cultural, morphological and pathogenicity. Culture and morphology showed variations in colony color, radial growth pattern and production of pycnidia and pycnidiospores. Significant differences (P < 0.05) in both pycnidia and pycnidiospores size among isolates were observed. Hence, the size of pycnidia and pycnidiospores of M. pinodes varied from 145 × 143 μm to 280 × 265 μm and from 11.5 × 2.3 μm to 22.5 × 6.3 μm respectively. Using the factor analysis, this revealed that the first principal component (pc) was more related to the growth and sporulation aspect, hence, the colony growth and both the pycnidia and pycnidiospore density were more related to the first pc, while the second pc contributed for the pycnidiospores size. The isolates were also evaluated for their pathogenicity on seven cultivars in controlled conditions. Cluster analysis based on disease rating on a scale of 1 to 5, indicated higher similarity coefficient. In addition, using Euclidian distances method, the clusters were subdivided at 70% of similarity in seven pathotype groups (PG). The two first pathotypes grouped the most isolates (70%), representing isolates from the four agro climatic regions. However, the members of same group were different in their cultural and morphological characteristics. A detailed study to investigate molecular and genetic basis of diversity is suggested.
Article 27 :Année de publication 2011
Titre Identification and Characterization of Functional and Technological Lactobacillus plantarum Strains Isolated from Raw Goat and Camel Milk Collected in Algeria
   
Revue, référence JOURNAL OF PURE AND APPLIED MICROBIOLOGY, J. Pure & Appl. Microbiol., 5(2). 553-566
   
Autres auteurs Boumehira Ali Zineddine
Mami Anas
Hamedi Amine Rizk
Henni Jamal Eddine
Kihal Mebrouk
   
Mots Clefs Lactobacillus plantarum Milk, Poultry, animals., Growth kinetics, Antagonism, Antibiotics, Probiotics
   
Résumé
   The role of lactic acid bacteria (LAB) in food preservation, prevention of poisoning, increased nutritional value and improved organoleptic quality of food, is indirectly related to food hungry from different continents and so the ensuring a sustainable development. This work identifies five strains Lb. plantarum, with an atypical growth at 45 °C. The results of technological test are satisfactory for industrial use. The strains are heat resistant, produce flavors and have a proteolytic activity. However, they do not produce dextran on MSE media. Strains of Lb. plantarum have a kinetics growth and acidification in MRS broth and skim milk at 30 °C, almost identical. But the strain Lb. johnsonii has a different kinetic growth and acidification. The best growth temperature for Lb. plantarum LbG22 strain was 37 °C. The strains Lb. plantarum LbC5 and LbC6 have the best antibacterial activity, they inhibited all tested strains. The Lb. plantarum LbG22 strain has the greatest inhibitory activity against the strains of Staphylococcus aureus, compared to other lactic acid strains studied. In mixed culture, growth of S. aureus ATCC 43300 was inhibited after 24h of culture with Lb. plantarum LbG22. Susceptibility to antibiotics was also evaluated with 33 antibiotics.
Article 26 :Année de publication 2011
Titre Advances of Molecular Markers Application in Plant Pathology Research
   
Revue, référence European Journal of Scientific Research, European Journal of Scientific Research. Vol.50 No.1 (2011), pp.110-123
   
Autres auteurs Benali Setti
Mohamed Bencheikh
Jamel Eddine Henni
Claire Neema
   
Mots Clefs Plant pathology, molecular markers, population genetics, PCR pyresequencing, TaqMan, taxonomy
   
Résumé
   During the last decade, genetics and molecular markers have gained an increasing presence in plant pathology. The purpose of this review is to highlight the most important molecular markers development that have been studied and reported recently. We have presented the most relevant published reports with emphasis on the use of the molecular markers for the population pathogen genetics. The principles, requirements, advantages, disadvantages are discussed in this review. Among the most popular markers employed in the plant pathology studies and research include Restriction Fragments Length Polymorphism (RFLP), Random amplified polymorphism DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeats (SSR), Internal Transcribed Sequence (ITS) and Single Nucleotide Polymorphism (SNP). The application and the development of theses markers were summarized in the following areas: mapping and tagging of genes, plant pathogen identification, Genetic structure, population genetics and genetic diversity, taxonomy and phylogeny.
Article 25 :Année de publication 2010
Titre Effet inhibiteur des espèces de lactobacillus isolées du lait cru de chèvre, sur la croissance de staphylococcus aureus.
   
Revue, référence CRASTRA, 7, 3 :1-10
   
Autres auteurs Anas Mami
Bettache Guessas
Jamal Eddine Henni
Mebrouk Kihal
   
Mots Clefs lait cru de chèvre, bactéries lactiques, interaction, Bactériocine, Staphylococcus
   
Résumé
   La contamination des aliments est un problème majeur pour le consommateur surtout dans la période estivale dans les pays chaud. L’exploitation des interactions bactériennes est un nouveau moyen pour lutter contre ces germes pathogènes. La détection de bactéries lactiques productrices de substances antimicrobiennes vis-à-vis des germes indésirables fait l’objet de ce travail. Les méthodes microbiologiques et biochimiques ont été utilisées pour identifier les bactéries présentant une activité antimicrobienne. Neuf isolats de bactéries lactiques ont été identifies à partir du lait cru de chèvre dans les régions ouest d’Algérie. Les espèces dominantes appartenant au genre Lactobacilles sont : Lb. rhamnosus, Lb. plantarum, Lb. casei, Lb. paracasei subsp. paracasei, Lb. acidophilus, Lb. delbrueckii subsp lactis, Lb. fermentum, Lactobacillus paraplantarum et Lactobacillus sakei subsp. sakei. L’étude des interactions a révélé la capacité de trois espèces Lb. plantarum (58), Lb. paracasei subsp. paracasei (55) et Lb. rhamnosus (68) à inhiber Staphylococcus aureus. En culture mixte après 24 heures, Lb. plantarum réduit considérablement la croissance de Staphylococcus aureus de 1,6 log et devient nulle après 72h. Les différents tests révèlent la nature protéique de cette substance impliquée dans l’inhibition de Staphylococcus aureus.
Article 24 :Année de publication 2010
Titre Activité Anti-Bactérienne de Lactobacillus plantarum isolée du lait crude chèvre d’Algérie vis à vis de Staphylococcus aureus
   
Revue, référence LES TECHNOLOGIES DE LABORATOIRE -,, Volume 5, N°21 .26-34
   
Autres auteurs Mami Anas
Hamedi Amine Rizk
Henni Jamal Eddine
Kerfouf Ahmed
Kihal Mebrouk
   
Mots Clefs lait cru de chèvre, bactéries lactiques interaction, bactériocine, Staphylococcus aureus, culture mixte
   
Résumé
   La contamination des aliments est un problème majeur pour le consommateur surtout durant la période estivale dans les pays méditerranéens L’exploitation des interactions bactériennes est un nouveau moyen pour lutter contre les germes indésirables. L’objectif de ce travail est la recherche des bactéries lactiques productrices de substances antimicrobiennes vis-à-vis des germes nuisibles. Les méthodes microbiologiques et biochimiques ont été utilisées pour identifier les bactéries présentant une activité antimicrobienne. Neuf isolats de bactéries lactiques ont été identifiés à partir du lait cru de chèvre dans les régions de l’Ouest algérien. Les espèces dominantes appartenant au genre Lactobacilles sont : Lb. rhamnosus, Lb. plantarum, Lb. casei, Lb. paracasei subsp. paracasei, Lb. acidophilus,Lb. delbrueckii subsp lactis, Lb. fermentum, Lb. paraplantarum et Lactobacillus sakei subsp. sakei. L’étude des interactions a révélé la capacité de trois espèces Lb. plantarum (58), Lb. Paracasei subsp. paracasei (55) et Lb. rhamnosus (68) à inhiber Staphylococcus aureus. En culture mixte, Lb. plantarum réduit considérablement la croissance de Staphylococcus aureus de 1,6 log après 12 h d’incubation et aucune croissance n’a été observée après 72h. Les différents tests utilisés révèlent la nature protéique de cette substance impliquée dans l’inhibition de Staphylococcus aureus.
Article 23 :Année de publication 2010
Titre Survival analysis to determine the length of latent period of Mycosphaerella pinodes on peas (Pisum sativum L.)
   
Revue, référence African Journal of Microbiology Research, Vol. 4(18), pp. 1897-1903.
   
Autres auteurs Benali Setti
Mohamed Bencheikh
Jamel Henni
Claire Neema.
   
Mots Clefs Cox regression, survival analysis, Mycosphaerella blight, Pisum sativum.
   
Résumé
   Blight caused by Mycosphaerella pinodes is one of the most devastating diseases of pea that causes yield losses of over 50% in some years and may cause total failure to the crop under epidemic conditions. During this study, a sensitive disease assessment and statistical tool was developed for Mycosphaerella blight on peas, the latent period was used to discriminate between different treatments. The time until appearance of the first pycnidia (latent period length) was recorded. Seedling that did not display any production of pycnidia structure during the 20 days period of observation was recorded as right censored observations. Using non parametric and semi parametric survival analysis, different hypothesis dealing with factors that might influence the latent period was tested. Survival analysis using Kaplan-Meier estimates and Cox proportional hazards were performed for data analysis. During these investigations, latent period was regressed against leaf wetness duration, pea cultivar, inoculum concentration, plant age and isolate aggressiveness. Both the Cox regression and Kaplan-Meier tests had shown the importance of leaf wetness duration, inoculums concentration and isolate aggressiveness on the survival times, thus, the median latent period length was respectively 15 and 16 for tn0203 and md0202. The median for the 3 leaf wetness was 14, 16 and 17 respectively for 06, 48 and 72 h LWD. Both the cultivar and plant age had no significant risk for the pycnidia structure appearance. Likewise, using the semi parametric Cox proportional hazard regression, the 2 covariates namely higher leaf wetness, higher inoculum dose with an aggressive isolate were all associated significantly with survival time. Hence, the hazard ratio was respectively 1.205 and 1.423 for LWD and inoculum concentration respectively.
Article 22 :Année de publication 2010
Titre Antibacterial Activity of Quaternary Ammonium Salt from Diethylaminoethyl Methacrylate.
   
Revue, référence E-J Chem., 2010, 7(S1), pp S61- S66
   
Autres auteurs Makhloufia Mohammed
Benaïssa Tahar
Derdour Aïcha
Henni Djamel Eddine
   
Mots Clefs Quaternary ammonium salis, DEAEMA, Antibacterial activity, MIC
   
Résumé
   A quaternary ammonium sait was synthesized from diethylaminoethyl methacrylate (DEAEMA) by quaternization with hexadecyl bromide. The resultant compound (Am-h) was characterized by 1-1:11( and NMR spectroscopy. lis bactericidal activity was evaluated by determining minimum inhibitory concentration (MIC) values and inhibitory zone diameter against gram positive bacteria (Streptococcus sp.) and gram negative bacteria such as Acenito baumannii, Klebsiella pneumoniae and Proteus sp. respectively. The results showed that the MIC values of the synthesised compound (Am-h) were 2. Itg/mL against Acenito baumannii, Klebsiella pneurnoniae, Proteus sp. and Streptococcus sp
Article 21 :Année de publication 2010
Titre Biological control of vascular wilt disease of tomato caused by Fusarium oxysporum f.sp lycopersici by Pseudomonas fluorescens, Bacillus cereus, Serratia marcescens and Trichoderma harzianium
   
Revue, référence Research Journal of Agronomy J, 4.2.31-32
   
Autres auteurs Nour Eddine Karkachi
Samia Gharbi2
Mebrouk Kihal1
Jamal Eddine Henni
   
Mots Clefs Fusarium, Biological control, Pseudomonas, Bacillus cereus, Trichoderma, Antagonisms inhibition
   
Résumé
   Evaluation of the antagonistic activity of three bacterial and a fungi with direct confrontation method and the filtrates culture against the growth of Fusarium oxysporum f.sp lycopersici showed the inhibition of the mycelia growth of Fusarium oxysporum f.sp lycopersici with Bacillus cereus, energized the low activity, and it was more significant with Serratia marcescens and Trichoderma harzianium for the 2nd day but with Pseudomonas fluorescens, it’s was for the 5th day . The filtrates of culture of these antagonists showed that only Serratia marcescens and Trichoderma have a rate of inhibition which varies between (40-95%), and of (20-30%) with Pseudomonas fluorescens
Article 20 :Année de publication 2009
Titre Effecte of pea cultivar, pathogene isolate, inoculum concentration and leafwetness duration on Ascochytae blight caused Mycosphaerella pinodes.
   
Revue, référence Phytopathol. Mediterr. / Phytopathology Mediterraneen, 48, 214-222.
   
Autres auteurs Setti Benali Bencheikh Mohamed Henni Jamel Neema Claire
   
Mots Clefs Pea , Leafwetness , Ascochytae, Mycosphaerella, pinodes
   
Résumé
   Mycosphaerella et l'isolat de microbe pathogène la période latente et la période d'incubation de la sévérité de microbe pathogène ou de maladie étaient quanti ? ED sur le pois (pisum sativum L.). Des jeunes plantes de deux cultivars largement développés de pois, en avant et de Merveille de Kelvedon, respectivement susceptibles et modérément résistants aux pinodes de M. ont été soumises à six durées d'humidité de feuille de 6, de 12, de 24, de 48 et de 72 h, et inoculées avec ? concentrations d'inoculum de ve, 2.5× 10 3, 4× 10 4, 3.5× 10 5, 4× 10 6, et 5.2× 10 7, afin de déterminer si les cultivars ont réagi différemment au M. isolats de pinodes inoculés dans des conditions identiques. L'augmentation de la durée de l'humidité de feuille et de la concentration d'inoculum a causé le signi ? la pente (P < 0.001) augmente dans la sévérité de la maladie dans chaque cultivar. La période d'incubation et la période latente ont diminué avec l'augmentation de la durée conidial d'humidité de concentration et de feuille. Généralement, cv a en avant eu un signi ? une période cantly plus courte d'incubation, et une sévérité de période et plus élevée latente de la maladie que cv Merveille de Kelvedon. Les isolats ont différé dans l'agressivité à des niveaux plus élevés de l'humidité de feuille (h) durée 48 et de concentration d'inoculum (4× 106), mais là n'était-il aucun signi ? interaction biseautée entre les isolats et la durée d'humidité de feuille, ou entre les isolats et la concentration d'inoculum. Les niveaux optima pour obtenir une infection cohérente et pour séparer aisément les cultivars susceptibles et partiellement résistants étaient une humidité de feuille de 48 h et une concentration en inoculum de 4× 10 6. L'étude a également prouvé que l'humidité continue de feuille pour 48 h était un seuil pour l'application des fongicides pour commander le mycète dans le cultivar susceptible.
Article 19 :Année de publication 2009
Titre Carbon dioxide production by Leuconostoc mesenteroîdes grown in single and mixed culture with Lactococcus lactis in skim milk
   
Revue, référence Scientific Research and Essay, Vol. 4 (11), pp. 1348-1353,
   
Autres auteurs Kihal .M Prevost .H Henni D. E. Benmechernene .Z Diviès .C
   
Mots Clefs Leuconostoc, mesenteroîdes, fermented milk, Lactococcus lactis Lactic; acetic and citric acid milk, kinetics growth
   
Résumé
   The effect of mixed culture of Leuconostoc mesenteroîdes subsp. dextranicum and Lactococcus lactis subsp. lactis (prot+) was investigated to achieve an optimal production of carbon dioxide. Only the strain of L. mesenteroîdes can produce carbon dioxide from lactose and citrate in milk. The influence of the initial concentration ration between the two strains on growth, carbon dioxide, L-lactate, acetic acid production and citrate used was studied. When the initial inoculum of L. lactis was 2.5 x 105 cfu/ml, the growth and evolved CO2 by L. mesenteroîdes (3 x 107 cfu/ml) increased, whereas high inoculum of L. lactis induced a decrease of growth and CO2 production by L. mesenteroides. In mixed culture, CO2 production continued after growth stopped, a partial uncoupling can be observed between growth and CO2 production. A shift of acetate production was observed in mixed culture and 25.6 mM was obtained, whereas 30.18 mM was obtained at the same time in pure culture of L. mesenteroîdes.
Article 18 :Année de publication 2009
Titre Phenotypic and Whole Cell Protein Analysis by SDS-PAGE for Identification of Dominants Lactic Acid Bacteria Isolated from Algerian Raw Milk.
   
Revue, référence World Journal of Dairy & Food Sciences, 4 (1): 78-87
   
Autres auteurs Fatima Ghazi Djamal Eddine Henni Zineb Benmechernene Mebrouk Kihal
   
Mots Clefs Lactic acid bacteria Leuconostoc, phenotypic method, SDS-PAGE, raw milk
   
Résumé
   Phenotypic identification of 21 representative strains revealed the presence of the following species: Leuconostoc mesenteroides subsp. dextranicum, Weissella paramesenteroides, Lactococcus lactis biovar. diacetylactis, Pediococcus acidilactici, Enterococcus faecium, Lactobacillus rhamnosus, Lactobacillus paracasei subsp. paracasei and Lactobacillus plantarum. In order to validate the previous results, whole cell protein patterns were obtained using Sodium Dodecyl Sulphate Gel Electrophoresis (SDS-PAGE) of these lactic acid bacteria strains, were analysed by calculating the coefficients of similarity (>100) for each two strains which were 80.7% and 78% for next couple of strains (6, 13) and (43, L4) that were identified phenotypically as Weissella paramesenteroides and Leuconostoc mesenteroides subsp. dextranicum, respectively. The coefficients of similarity (>100) between one strain of Leuconostoc mesenteroides and one strain of Weissella paramesenteroides were 36%, 48%, 44.4%, 48.6%, 44.4% and 48.6%, for the following couples (L4, 13), (L4, 6), (43,13), (43,6), (27,13) and (27, 6), respectively, in agreement with distant phylogenetic relationship between Leuconostoc mesenteroides and Weissella paramesenteroides. The SDS-PAGE method allowed clarifying some ambiguous points in phenotypic identification. It had corroborate, complete and correct phenotypic identification, although, further study is required to accurate it.
Article 17 :Année de publication 2008
Titre Evaluation de la qualité sanitaire des eaux brutes de consommation : cas de la ville de Béni-Abbès du sud ouest algérien.
   
Revue, référence Géographie et Aménagement, Bulletin, A.G.A.T, 13 : 67-77.
   
Autres auteurs Bengarnia Benmerine
Al Aboudi Abdul Kadhum
*Kihal Mebrouk
   
Mots Clefs Zones arides, eau de consommation, qualité de l’eau, Foggara, puits, Béni-Abbès
   
Résumé
   Le développement de la ville de Béni-Abbès, sous le poids de la démographie et des activités administratives et commerciales, a entraîné le détournement des eaux réservées à l’irrigation des sols de la palmeraie. L’analyse physico-chimique et microbiologique des ressources d’eau Ain Sidi Othmane, des deux puits (communal, forage1) et les anciennes foggaras ont fait l’objet de cette étude. Les résultats montrent en évidence que la source principale de Ain Sidi Othmane reste une source eaux de qualité sanitaire et hygiénique irréprochables avec un taux de résidus secs le plus faible. Les nouveaux puits communal et forage 1 présentent aussi une eau contenant un taux faible de sel par rapport à celui cité dans la bibliographie. En revanche les eaux de toutes les Foggaras sont inaptes à la consommation humaine par la présence de Clostridium. Par ailleurs, la foggara 1 contient la concentration la plus élevée en résidus secs qui est de 2,325 g/l. L’absence de germes dans la foggara 10 nous nous incite à approfondir les analyses physico-chimiques. Les sources destinées aux réseaux de distribution doivent être surveillées.
Article 16 :Année de publication 2008
Titre Kinetic of carbon dioxide production by Leuconostoc mesenteroides in single and mixed culture with Lactocccus lactis in skimmed milk.
   
Revue, référence Research Journal of Dairy Sciences, 2 (3): 30-35-.
   
Autres auteurs Kihal M.
Prevost H.
Benmechernene Z.
Diviès C.
   
Mots Clefs Leuconostoc mesenteroides, Lactococcus lactis, milk, lactic acid, acetic acid, citric acid, growth, kinetics fermented milk
   
Résumé
   The effect of mixed culture of Leuconostoc mesenteroides subsp. dextranicum and Lactococcus lactis subsp. lactis (prot+) was investigated to achieve an optimal production of carbone dioxide. Only the strain of Leuconostoc mesentroides can produce carbon dioxide from lactose and citrate in milk. The influence of the initial concentration ration between the 2 strains on growth, carbon dioxide, L-lactate, acetic acid production and citrate used was displayed. When the initial inoculum of Lactococcus lactis was 2.5 105 cfu mLG1, the growth and evolved CO by Leuconostoc mesenteroides (3 10 cfu mLG ) increased. Whereas, high inoculum 2 7 1 of Lactococcus lactis induce a decrease of growth and CO production by Leuconostoc mesenteroides. In 2 mixed culture CO production continued after growth stopped, a partial uncoupling can be observed between 2 growth and CO production. A shift of acetate production was observed in mixed culture and 25.6 mM was 2 obtained, whereas 30.18 mM was obtained at the same time in pure culture of Leuconostoc mesenteroides.
Article 15 :Année de publication 2008
Titre Antimicrobial activity of Lactobacillus species isolated from Algerian raw goat’s milk against Staphylococcus aureus.
   
Revue, référence World Journal of Dairy & Food Sciences, 3 (2): 39-49..
   
Autres auteurs Mami Anas
Kihal Mebrouk.
   
Mots Clefs Raw goats’ milk, Lactic acid bacteria, Lactobacillus plantarum, Interaction, Bacteriocin, Staphylococcus, Mixed culture
   
Résumé
   Food contamination by Staphylococcus aureus is a major problem for consumer’s health in Algeria, especially during the summer period. The use of bacterial interactions is a new way to limit the pathogenic germs growth. Detection of antimicrobial substances produced by lactic acid bacteria against the undesirable germs is the objective of this work. Microbiological and biochemical methods were used to identify lactic acid bacteria having an antimicrobial activity. The 08 isolates of lactic acid bacteria obtained from raw goats’ milk n western Algeria's areas were identified. The dominant species belonging to the Lactobacillus genera are: Lb. rhamnosus, Lb. plantarum, Lb. casei, Lb. paracasei subsp. paracasei, Lb. acidophilus, Lb. delbrueckii subsp lactis, Lb. fermentum, Lactobacillus paraplantarum and Lactobacillus sakei subsp. sakei. The interactions study revealed that three lactobacilli species: Lb. plantarum (58), Lb. paracasei subsp. Paracasei (55) and Lb. rhamnosus (68) are able to inhibit Staphylococcus aureus’ growth. In mixed culture after 24 h, Lb. plantarum reduces the growth of Staphylococcus aureus by 1.6 log and this latter bacteria was not found after 72h. The various tests used reveal the proteinic nature of the substance which was responsible of the growth inhibition of Staphylococcus aureus. The ecological adaptation and growth characteristics of cultures of Lb. plantarum in food product will determine their effectiveness as biocontrol agent in dairy foods.
Article 14 :Année de publication 2008
Titre Effect of pea cultivarm pathogen isolate inoculum concentration and leaf wetness duration on ascochyta blight caused by Mycosphaerella pinodes
   
Revue, référence Phytopathol. Mediterr. / Phytopathology Mediterraneen, 47. 214-222.
   
Autres auteurs Setti Benali
Bencheikh Mohamed
Neema Claire
   
Mots Clefs Disease severity, Incubation period, Pisium sativum
   
Résumé
   The effect of host leaf wetness duration Mycosphaerella pinodes inoculum concentration and pathogen isolate on the latent period and the inoculum period of the pathogen or deases severity were quatified on pea Pisium sativum…..
Article 13 :Année de publication 2008
Titre Comparative aggression of Mycosphaerella pinodes on peas fro; different regions in western Algeria
   
Revue, référence Phytopathol. Mediterr. / Phytopathology Mediterraneen, 48. 00-00.
   
Autres auteurs Setti Benali
Bencheikh Mohamed
Neema Claire
   
Mots Clefs Disease severity, Incubation period, Pisium sativum, HCA PCQ, Aggressivity group
   
Résumé
   Mycosphaerella blight caused Mycosphaerella pinodes deases severity were quatified on pea Pisium sativum…..
Article 12 :Année de publication 2007
Titre carbon dioxide production by Leuconostoc mesenteroides in single and mixed culture with Lactocccus lactis in skimmed milk.
   
Revue, référence World Journal of Dairy & Food Sciences, 2 (2): 62-68.
   
Autres auteurs Kihal M.
Prevost H.
Benmechernene Z.
Diviès C
   
Mots Clefs Leuconostoc mesenteroides, Lactococcus lactis, milk, lactic acid , acetic acid, citric acid, growth kinetics, fermented milk
   
Résumé
   The effect of mixed culture of Leuconostoc mesenteroides subsp. dextranicum and Lactococcus lactis subsp. lactis (prot+) was investigated to achieve an optimal production of carbone dioxide. Only the strain of Leuconostoc mesentroides can produce carbon dioxide from lactose and citrate in milk. The influence of the initial concentration ration between the 2 strains on growth, carbon dioxide, L-lactate, acetic acid production and citrate used was displayed. When the initial inoculum of Lactococcus lactis was 2.5 105 cfu mLG1, the growth and evolved CO by Leuconostoc mesenteroides (3 10 cfu mLG ) increased. Whereas, high inoculum 2 7 1 of Lactococcus lactis induce a decrease of growth and CO production by Leuconostoc mesenteroides. In 2 mixed culture CO production continued after growth stopped, a partial uncoupling can be observed between 2 growth and CO production. A shift of acetate production was observed in mixed culture and 25.6 mM was 2 obtained, whereas 30.18 mM was obtained at the same time in pure culture of Leuconostoc mesenteroides.
Article 11 :Année de publication 2006
Titre Phenotypic identification and whole cell protein analysis by sds-page of dominants lactic acid bacteria isolated from Algerian raw milk.
   
Revue, référence Journal Algerien des Zones Arides, 5 : 25-35.
   
Autres auteurs GHAZI F.Z.
BENMECHERNENE
Z
AGGAD H
GUESSAS B.
MOUSSA
BOUDJAMAA
M. KIHAL
   
Mots Clefs Lactic acid bacteria, identification, phenotypic method, SDS-PAGE, raw milk, Leuconostoc
   
Résumé
   Phenotypic identification of 21 representative strains revealed the presence of the following species: Leuconostoc mesenteroides subsp. dextranicum, Weissella paramesenteroides, Lactococcus lactis biovar. diacetylactis, Pediococcus acidilactici, Enterococcus faecium, Lactobacillus rhamnosus, Lactobacillus paracasei subsp. paracasei and Lactobacillus plantarum. In order to validate the previous results, whole cell protein patterns were obtained using Sodium Dodecyl Sulphate Gel Electrophoresis (SDS-PAGE) of these lactic acid bacteria strains, were analysed by calculating the coefficients of similarity (>100) for each two strains which were 80.7% and 78% for next couple of strains (6, 13) and (43, L4) that were identified phenotypically as Weissella paramesenteroides and Leuconostoc mesenteroides subsp. dextranicum, respectively. The coefficients of similarity (>100) between one strain of Leuconostoc mesenteroides and one strain of Weissella paramesenteroides were 36%, 48%, 44.4%, 48.6%, 44.4% and 48.6%, for the following couples (L4, 13), (L4, 6), (43,13), (43,6), (27,13) and (27, 6), respectively, in agreement with distant phylogenetic relationship between Leuconostoc mesenteroides and Weissella paramesenteroides. The SDS-PAGE method allowed clarifying some ambiguous points in phenotypic identification. It had corroborate, complete and correct phenotypic identification, although, further study is required to accurate it.
Article 10 :Année de publication 2006
Titre A new manometric method for measuring carbon dioxide production by dairy starter cultures, a case of Leuconostoc mesenteroides.,
   
Revue, référence Afr. J. Biotechnol / African Journal of Biotechnology, 5, (4) : 378-383.
   
Autres auteurs Kihal M.
Prévost H.
Diviès C.
   
Mots Clefs Leuconostoc sp, CO2 production, lactic acid, milk
   
Résumé
   This paper describe a new technique for carbon dioxide (CO2) measurement based on the displacement of acidifying liquid in burette at constant pressure. It was adapted to allow the growth of Leuconostoc sp. On semi-synthetic media and milk. A highly precise measurement was obtained using the chemical reaction of sodium carbonate with hydrochloric acid. This method has been proven to present an excellent linear response from 0 to 0.895 mM of CO2 with a high sensitivity. With cultures in milk medium, kinetics of CO2 evolved by Leuconostoc mesenteroides subsp. dextranicum L4 were higher than L. mesenteroides subsp. mesenteroides 19D and L. mesenteroides subsp. cremoris Ec195 grown on milk.. The use of L. mesenteroides subsp. dextranicum L4 and their variants Lac(-)Cit(+), Lac(+)Cit(-) and Lac(-)Cit(-) showed that the CO2 production correlated to the substrate utilization. The curve of CO2 measured was close to the amount of CO2 calculated from lactose and citrate used. The CO2 production rate was higher with the parental strain (6.5 mM/ h) than with the variants. The amount of CO2 produced was always close to the theoritical value, and was also correlated to the growth rate and substrates utilization by L. mesenteroides. The volume of CO2 produced by Leuconostoc in milk was highly correlated with the D-lactate production (r = 0.995). This method could be used routinely for the evaluation and the selection of bacteria having potential ability for CO2 production.
Article 09 :Année de publication 2006
Titre Proteolytic activity of cultivable lactic acid bacteria isolated from Algerian raw goat’s milk.
   
Revue, référence World Journal of Dairy & Food Sciences, 1 (1): 12-18.
   
Autres auteurs Moulay. M.
Aggad H.
Benmechernene Z.
Guessas B.
Kihal M.
   
Mots Clefs Lactic acid bacteria, proteolysis, caseins, goat's milk
   
Résumé
   The proteolytic systems play an essential role in nitrogen metabolism of lactic acid bacteria in milk. The extracellular cell wall-bound proteinase is a key enzyme in this system, in which its activity is necessary for the growth of lactic acid bacteria in milk by initiating the breakdown of casein to smaller peptides. The screening of the proteolytic strains has been achieved on three solid media YMA, PCA and FSDA. The strain of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis, biovar. diacetylactis and the Enterococcus were tested. Lactococcus lactis produced a large hydrolysis zone more than 10 mm of diameter. The concentration of 3 and 5% of inoculum produced the highest acidity which was superior to 60°D. The proteolytic strains of Lactococcus lactis subsp. lactis biovar. diacetylactis at 3% of inoculum showed a maximal acidification rate 5°D /h and 0.3 unit pH/h. The strongest proteolytic strain Lactococcus lactis subsp. lactis biovar. diacetylactis produced an acidification rate of 8°D/h and a pH rate which decreases to 0.28 unit of pH/h with a final pH value of 4.29. The rate of casein hydrolysis by the original Lactococcus lactis subsp. lactis biovar. diacetylactis (90 mg hG1) was three times higher. The proteolytic activity of this strain can be exploited for the selection of performante lactic acid bacteria for the Algerian dairy industry.
Article 08 :Année de publication 2005
Titre Identification of cultivable bifidobacterium species isolated from breast-fed infants faeces and their technological properties for potential use as a probiotic cultures..
   
Revue, référence Afr. J. Biotechnol / African Journal of Biotechnology, 4 (5) : 422-430.
   
Autres auteurs Hadadji M Benaama R.
Saidi N.
Kihal M.
   
Mots Clefs Lactic acid bacteria, Bifidobacterium, probiotic, fermented milk, growth, survival
   
Résumé
   Gastrointestinal microflora exerts a high impact on its host mainly by performing a great variety of metabolic activities, protecting the host against colonization by pathogen and stimulating the gut immune system. The integration of bifidobacteria in dairy products is of interest for reinstalling the intestinal microflora. The mother's milk is a prebiotic factor which stimulate the bifidobacteria growth in vivo. Results obtained in west Algeria area show that the number of bifidobacteria reach 26 109 cfu/g in breast-fed infant feces. The microbiological, fructose–6-phospho-ketolase and the biochemical tests have revealed Bifidobacterium strains. The most prevalent isolates belong to the species Bifidobacterium longum (75%) and Bifidobacterium breve (25%). In order to evaluate the potential use as a starter culture, the occurrence of some selected properties such as growth and survival in fermented milk were investigated. These strains have a fast growth in glucose, galactose and lactose media compared to reference species. All isolates studied are sensitive to the pH 8. The B. breve strain support the conservation conditions in pure culture better than in mixed culture with Streptococcus thermophilus or Lactobacillus acidophilus.
Article 07 :Année de publication 2005
Titre Characterization of algrian raw camel’s milk identification of dominant lactic acid bacteria and proteins analysis..
   
Revue, référence Journal algérien des zones Arides, (4): 01-09.
   
Autres auteurs Saidi D.
Kihal M.
Hamama A.
Chekroun A.
Kheroua O.
   
Mots Clefs Camel’s milk, Lactic Acid Bacteria, Proteins, Acidification
   
Résumé
   The chemical composition of Algerian raw camel’s milk is slightly rich in fat 34.4 +- 2.8 g.l-1, proteins 33.1 +- 2.1 g.l-1, lactose 45.1 +- 3.1 g.l-1, ash 8.15 +- 0.15 g.l-1 and total solids 122.6 +- 0.12 g.l-1. This composition varied by several factors such as feeding, breeds, milk yielding and the health of the animal. Our results showed that total casein proteins were higher than whey proteins. SDS-PAGE showed that casein proteins of camel’s milk and cows’ milk have the same molecular weight, about 24 kDa. The whey proteins of camel’s milk were presented by 5 bands. Compared with cow’s milk, camel’s milk contents a little amount of β-lactoglobuline. The pH of raw milk decrease from 6.5 to 5.4 after 72 h of incubation at 30°C. The total count of lactic acid bacteria can reach 200 x 106 cfu ml-1 in MRS medium. Fourty strains of lactic acid bacteria were isolated from camel milk. The mesophilic group were represented by Lactococcus lactis subsp. lactis biovar. diacetylactis which is the dominant strain, L. lactis subsp. Lactis, L. lactis subsp. cremoris and L. lactis subsp. lactis biovar. diacetylactis produce more than 100 mM of lactic acid after 24 h of incubation at 30°C. The thermophilic strains were represented by two species, Streptococcus thermophilus and Enterococcus faecalis. From a technological point of view, the camel milk can be used as a source of new starters in the development of the industrial manufacture of these strains
Article 06 :Année de publication 2004
Titre Identification and technological properties of lactic acid bacteria isolated from raw goats milk of four Algerian races.
   
Revue, référence Food Microbiol, 21. 5 : 579-588.
   
Autres auteurs Badis A.
Guetarni D.
moussa Boudjema B.
Kihal M
   
Mots Clefs Lactic acid bacteria, Raw milk, Goat, Technological, characteristics
   
Résumé
   The characteristics of725 lactic acid bacteria isolated from raw goat milk off our Algerian races were studied. They were phenotypically classified as Lactobacillus (31.6%), Lactococcus (28.4%), Leuconostoc (22.2%), Streptococcus (13.7%) and Pediococcus (4.1%). No major differences were observed as to the distribution of genus isolates in the milk of three races (Makatia, Makatia-Chamia and Kabyle). However, the number ofisolates ofboth Leuconostoc and Lactococcus predominated in Arabia milk while the samples collected from the other races mostly contained lactobacilli isolates. The phenotypic and biochemical analyses gave a diversity ofspecies (28 presumptive species). The most abundant species were Streptococcus thermophilus (46 isolates), Lactobacillus helveticus (45 isolates), Lactobacillus plantarum (30 isolates), Lactobacillus delbruecki subsp. bulgaricus (26 isolates), Lactobacillus brevis (25 isolates) and Lactococcus lactis subsp. lactis (25 isolates). The technological properties of the isolates were as follows: 38.6% of isolates showed a fast acidifying rate (more than 60_D during 18 h ofincubation); 25.9% ofisolates had a high proteolytic capacity (more than 6 ppm leucine/72 h) and 14.1% of isolates had a high diacetyl production (more than 0.8 ppm diacetyl/16 h). Nine isolates were selected to prepare yoghurt and cheese. Sensory tests performed by a trained panel revealed a pleasant yoghurt prepared with mixed culture St. thermophilus 16 TMC+L. helveticus 20TMC. Cheese prepared with isolates L. lactis subsp. Lactis biovar diacetylactis 19 MCA and L. lactis subsp. lactis 10 MCM had a very good sensory quality and a pleasant taste.
Article 05 :Année de publication 2004
Titre Characteristics of cultivable lactic acid bacteria isolated from Algerian raw goat’s milk and evaluation of their technological properties
   
Revue, référence Food Microbiol, 21. 3 : 343-349.
   
Autres auteurs Badis A.
Guetarni D.
moussa Boudjema B.
Tornadijo E.
Kihal M.
   
Mots Clefs Lactic acid bacteria, Goat’s milk, Identification, Technological, characterization
   
Résumé
   One hundred and fifty-eight strains of lactic acid bacteria isolated from Algerian raw goat’s milk were identified and technologically characterized. Five genera were found: Lactobacillus (50.63%), Lactococcus (25.94%), Streptococcus (14.56%), Leuconostoc (7.59%) and Pediococcus (1.26%). The predominant species were Lactococcus lactis (32 strains), Streptococcus thermophilus (23 strains), Lactobacillus bulgaricus (19 strains), Lb. helveticus (16 strains) and Lb. plantarum (14 strains). Approximately 39% of the lactic acid bacteria isolated produced more than 0.6% lactic acid (w/v) after 18 h of incubation, and belonged to the Lactococcus and Lactobacillus genera. The highest proteolytic activity was approximately 3mg tyrosine l_1 for mesophilic strains and nearly 5mg tyrosine l_1 for thermophilic lactobacilli after 72 h. High aromatic activity (more than 0.8 mg diacetyl l_1 after 16 h) was detected in 14% of the strains. Nine strains were used to make dairy products (a yoghurt-like product and Edam-type cheese) on a pilot scale in the laboratory. The best-liked organoleptic characteristics were noted in a yoghurt produced with a mixed culture made up of S. thermophilus (strain 16TMC+) and Lb. helveticus (strain 20TMC) and in a cheese made with a starter composed of Lc. lactis subsp. lactis (strain 10MCM) and L. lactis subsp. lactis (V.P. +) (strain 19MCM).
Article 04 :Année de publication 2002
Titre Microbiological quality of drinking water at arid zones in Adrar South Algeria
   
Revue, référence J. Alg. Reg. Arides / Journal Algérien des Régions Arides, 01 : 45-50.
   
Autres auteurs Kihal M.
Boutiba Z.
Al
Aboudi AK.
   
Mots Clefs Drinking water, coliforms, bacteria, foggara, Adrar, Algeria, arid zones.
   
Résumé
   From ancient times the arid zones of Sahara, south Algeria, stay in a good state without signified contamination with biological and chemical matters. At the last five decades, the zones show large movement of population, therefore that became a new source of microbial and chemical contamination. The microbiological quality of drinking water has been evaluated in 28 samples from underground wells and foggara water which were analyzed. The essential microflora : aerobic bacteria (29,33 ± 0,44) cfu per100 ml, total coliforms (6,6 ± 0,15) cfu per 100 ml, fecal coliforms (2,33 ± 0,42) cfu per 100 ml and fecal streptococci (1,51 ± 0,66) cfu per 100 ml. The pH ranged from 6,22 to 7,34. Likewise, in 100 % of drinking water more than 71,42 % was non drinking water.
Article 03 :Année de publication 2002
Titre Phenotypic identification of dominant homofermentative lactic acid bacteria isolated from Algerian whole camels milk.
   
Revue, référence Selected Research Papers, 33 :10-16.
   
Autres auteurs Kihal M
Chekroun A.
Saidi D
Kheroua O
   
Mots Clefs Camels’ milk, lactic acid bacteria, proteins, growth, acidification, identification
   
Résumé
   The chemical composition of Algerian raw camel’s milk is slightly rich in fat 3.44 ± 0.28%, proteins 3.31 ± 0.21%, lactose 4.51 ± 0.31, ash 0.815 ± 0.015% and total solids 12.26 ± 0.012%. This composition varied by several factors such as feeding, breeds, milk yielding and the health of the animal. Our results showed that total casein proteins were higher than whey proteins (57% and 43%) respectively. SDS-PAGE showed that casein proteins of camel’s milk and cow’s milk have the same molecular weight about 45 kDA. The whey proteins of camel’s milk were presented by 5 bands. The pH of raw milk decrease from 6.5 to 5.4 after 72h of incubation at 30°C. The total count of lactic acid bacteria can reach 200 x 106 cfu ml-1 in MRS medium. Forty strains of lactic acid bacteria were isolated from camel milk. The isolates were divided into two groups mesophilic and thermophilic. The mesophilic group was represented by Lactococcus sp., Lactococcus lactis subsp. lactis biovar. diacetylactis is the dominant strain and represent 60% of the total acid bacteria. Lactococcus lactis subsp lactis biovar. diacetylactis produces more than 100 mM of lactic acid after 24 h of incubation at 30°C. The termophilic strains were represented by two species Streptococcus thermophilus and Enterococcus feacalis. From a technological point of view, the camel milk can be used as a source of new starters in the development of the industrial manufacture of these strains.
Article 02 :Année de publication 2002
Titre Caractérisation des bactéries lactiques isolées du lait cru de chèvre des regions arides
   
Revue, référence J. Alg. Reg. Arides / Journal Algérien des Régions Arides, 1 : 01-11.
   
Autres auteurs Saidi N.
Guessas B.
Bensalah F.
Badis A.
Hadadji M.
Prevost H. KIHAL M
   
Mots Clefs Lait de chèvre, bactéries lactiques, identification, pouvoir acidifiant, Protéolyse
   
Résumé
   La diversité et la densité des bactéries lactiques du lait cru de chèvre des zones arides de l’ouest d’Algérie ont fait l'objet d’analyses phénotypiques, physiologiques et biochimiques. L'étude de 206 souches de bactéries lactiques a révélé la présence de 115 de formes cocci et 91 bâtonnets. Les cocci sont représentés par les genres Lactococcus (76,16%), Streptococcus thermophilus (14,78%) et Leuconostoc (8,6%). Lactococcus lactis subsp lactis est l’espèce dominante. Les espèces de lactobacilles détectées sont curvatus (25,25%), helviticus (10,98%), plantarum (9,89%), reuteri (9,89%), casei (7.69%), brevis (5,49%), bulgaricus (5,49%), paracasei (4,39%) et acidophilus (2,19%). Deux souches de Lactococcus lactis subsp lactis possèdent une activité protéolytique considérable. Le pouvoir acidifiant obtenu par les différentes souches testées en milieu lait se situe entre 42,2 mM et 110,6 mM en culture pure. En revanche, l’évaluation de la cinétique d’acidification en culture mixte fait apparaître différentes interactions positives et négatives.
Article 01 :Année de publication 2000
Titre تحديد المجامع المتكاملة خضريا عند عشيرة فطر Fusarium oxysporum f.sp. albedinis عزلت في منطقة بني عباس بالجهة الغربية من الجزائرٍ.
   
Revue, référence مجلة مؤتة للبحوث, مجلة مؤتة للبحوث و ألدراسات : مجلد 15 ، عدد 3.
   
Autres auteurs
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Mots Clefs التكامل الخضري. Fusarium oxysporum . الذبول الفيوزارى ,
   
Résumé
   درس التكامل الخضري عند عزلات لعشيرة فطر (Fusarium oxysporum ) تم الحصول عليها من نباتات طماطم مصابة بالذبول الفيوزارى ومن التربة المجاورة لها، و اورمات مخبرية مرجعية. الدراسة تمت عن طريق الاندماج الخلوى لكل العزلات الطافرة للانزيم المرجع للنترات. تم تحديد مجموعة متكاملة خضريا (VCG) تضم عزلات متجانسة ممرضة تنتمي كلها إلى (albedinis .Fusarium oxysporum f.sp ، اما العزلات الباقية فهي غير متكاملة ذاتيا و غير متكاملة مع الأفراد الاخرى مكونة بدلك مجامع خضرية بعدد العزلات المختبرة.



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