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KIHAL Mebrouk
Microbiologie Appliquée - LMA
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Article119 :Année de publication 2017
Titre Generation of bioethanol from common date byproducts, “Teggaza and Lebghel” in Southern Algeria
   
Revue, référence African Journal of Biotechnology, Vol. 16(1), pp. 41-50, 4 ( DOI: 10.5897/AJB2016.15369)
   
Autres auteurs Ahmed Boulal Mabrouk Kihal Cherif Khelifi Boudjemâa Benali
   
Mots Clefs Algerian Sahara alcoholic fermentation, bioethanol, bakery yeast, dates by-product, distillation
   
Résumé
   Date by-products constitute the principal food for the oasis populations in Middle East and North Africa. Dates contents consist of 70 to 80% of reducing sugars, and do not require an intensive energy and labour for thermophysical pre-treatment. They can serve as a good feedstock for bioethanol generation through fermentation and distillation. Algeria is among the top sixth producers of dates in the world with more than 250,000 tons/year; from these, more than 30% can be lost for different reasons and may be of low quality. In the laboratory, after an alcoholic fermentation of the substrate of the date varieties, Teggaza and Lebghel (T & L) using bakery yeast at 30°C for 72 h, the distilled and rectified date juice generated the highest ethanol ( 88° and 90°) with acceptable productions of 2.5 and 2.78 mL/kg/h, and assessed scale efficiencies of 23.57 and 26.2%. This is unlike the one (ethanol; 50%) directly generated by chemical reaction using the same quantity of sugar. The efficiencies that were obtained seem satisfactory and encourage the great scaling development of bioethanol generation using date waste biomass abundant in Algerian Sahara
Article118 :Année de publication 2017
Titre Optimization of DNA extraction from the Algerian traditional date’s product “Btana”
   
Revue, référence Applied Biology in Saharan Areas, Vol. 1, N. 1, p. 23-32
   
Autres auteurs AAbekhti S. Dgeghati B. Taminau M. Kihal G. Daube
   
Mots Clefs Traditional foods Dates Btana DNA extraction CTAB.
   
Résumé
   Btana is traditional preservation method that can sustain date’s supply during many years in comparaison to the commercial storage methods. However no scientific informations are available about biological factors that contribute to the successful of this method. Bacterial communities are a major key in preservation of many foods. Culture independent techniques are the most powerful tools to enhance bacterial diversity studies, but their efficiencies start with DNA extraction step. Therefore we have studied 3 protocols of DNA extraction from 11 Btana samples to evaluate their yield in total microbial DNA recovery. Protocols were based on a commercial kit DNeasy (QIAGEN, Germany) and two modified CTAB extraction methods (combined CTAB-DNeasy protocol, modified CTAB protocol) using polyvinyl pyrrolidone (PVP) and treatment with high salt solution (5M NaCl). Protocols were compared for quantity of DNA extracted using NanoDrop® ND-1000 Spectrophotometer and quality of DNA by 260/280 nm absorption ratio. The total extracted DNA was cheeked by PCR amplification of the 16S rRNA and visualized by electrophoresis on agarose gel (0.8%). Results showed that CTAB modified method provide the best DNA yield; however purification with NucleoSpin® Kit (Clontech, UK) was mostly needed for amplifying the DNA template. DNeasy kit protocol gave an amplified high quality DNA, but poor yields were obtained from date samples.
Article117 :Année de publication 2017
Titre Evaluation of technological properties of Leuconostoc mesenteroîdes (V1) strain isolated from Algerian goat’s milk
   
Revue, référence Advances inEnvironmental Biology, 11(1),: 26-38
   
Autres auteurs Amel Benyoucef Nabila Hansal
Mohammed Amine El hadj Said Hassan Bentabet Mebrouk Kihal Zineb Benmechernene
   
Mots Clefs In vitro goat„s milk probiotic Leuconostoc
   
Résumé
   This study was to evaluate probiotic characteristics by in vitro tests of Leuconostoc mesenteroides strain (V1) isolated from Algerian goat‟s milk collected from El Bayadh which is located in the west of Algeria. This strain V1 were identified by phenotypic and bioch emical methods, were tested for their antimicrobial activity against Staphylococcus aureus, Listeria innocua, Listeria ivanovii and was evaluated for certain properties relevant to probiotic including : acid pH, bile salts, pepsin resistance, hemolytic activity and antibiotics sensitivity. The results revealed the strain V1 showed antagonistic activity against Staphylococcus aureus, Listeria innocua and Listeria ivanovii, due to a production of proteinous nature substances.The strain were resistant to pH 3 and 4, bile salts at 0.5%, 1% and 2% and pepsin at pH 3. Was γ- hemolytic and was susceptible to four antibiotics : Chloramphenicol, pristinamycin, Clindamycin and Licomycin. Leuconostoc mesenteroides strain V1 showed desirable properties as potential probiotic strains, which can be considered the strain V1 as suitable probiotic candidate.
Article116 :Année de publication 2017
Titre Microbiological Characterization Of Algerian Traditional Cheese "Klila
   
Revue, référence Journal of Purity, Utility Reaction and Environment, Vol.6 No.1, February 2017, 1- 9
   
Autres auteurs Benlahcen Kheira
ne
Mahamedi Alla Eddi Sadeki Ines Feriel Djellid Youssef
Kihal Mebrouk
   
Mots Clefs Dairy products Klila Consumptio microbiological analysis bacterial pathogens anti-pathogenic effect
   
Résumé
   Traditional methods of fermented dairy products manufacturing have been transferred from generation to another. Those skills are part of the rich heritage of traditional Algerian food technology. Among a multitude of dairy traditional products, the Klila cheese seems to be the most produced and consumed in Algeria. This traditional cheese is usually intended for daily domestic consumption provincial populations have already affirmed its therapeutic use. To reveal the characteristics that distinguish the Klila, we have carried out a microbiological analysis, aims to evaluate the amount of the major microbial groups and to verify the presence of harmful bacteria for consumer’s health. For that, eight samples from separate areas in Algeria have been collected (Biskra, M'sila, Tiaret,Sidi Bel Abbès, El Bayadh and Naâma). The load in FAMT finded was 108 CFU/g reflecting the ri and contaminating microorganisms. However, only two samples contained total coliforms with an average of 103CFU/g. Lactic acid bacteria as main actors of lactic fermentation mark their presence with a rate reaching the 106 CFU/g, which °D and a pH value of 4,63. The load noted for yeasts and molds was 108 CFU/g. On the other hand, Staphylococcus aureus, Salmonella, fecal coliforms and clostridia were totally absent in all studie samples.Finally, despite the presence of an important microbial load, Klila stays away from very terrifying traditional products, especially when we know that this cheese has been consumed for centuries by the population without any concern, besides the generally recognized as safe and benefice with their known anti pathogenic effect
Article115 :Année de publication 2017
Titre Rheology and bioactivity of high molecular weight dextranssynthesised by lactic acid bacteria
   
Revue, référence Carbohydrate Polymers, 174 (2017) 646–657
   
Autres auteurs Kenza Zarour MaGoretti Llamas Alicia Prieto Patricia Ruas
Madiedo Maria Teresa Due˜nas Pilar Fernandez de Palencia Rosa Azna Mebrouk Kihal Paloma Lopez
   
Mots Clefs Exopolysaccharides Dextran Lactic acid bacteria Leuconostoc mesenteroides Rheological properties Immunomodulation Adhesion
   
Résumé
   Dextrans synthesised by three Leuconostoc mesenteroides strains, isolated from mammalian milks, werestudied and compared with dextrans produced by Lc. mesenteroides and Lactobacillus sakei strains iso-lated from meat products. Size exclusion chromatography coupled with multiangle laser light scatteringdetection analysis demonstrated that the dextrans have molecular masses between 1.74 × 108Da and4.41 × 108Da. Rheological analysis of aqueous solutions of the polymer revealed that all had a pseudo-plastic behaviour under shear conditions and a random, and flexible, coil structure. The dextrans showedat shear zero a difference in viscosity, which increased as the concentration increased. Also, the puri-fied dextrans were able to immunomodulate in vitro human macrophages, partially counteracting theinflammatory effect of Escherichia coli O111:B4 lipopolysaccharide.During prolonged incubation on a solid medium containing sucrose, dextran-producing bacteriashowed two distinct phenotypes not related to the genus or species to which they belonged. Coloniesof Lc. mesenteroides CM9 from milk and Lb. sakei MN1 from meat formed stable and compact mucoidcolonies, whereas the colonies of the other three Leuconostoc strains became diffuse after 72 h. This dif-ferential behaviour was also observed in the ability of the corresponding strains to bind to Caco-2 cells.Strains forming compact mucoid colonies showed a high level of adhesion when grown in the presence ofglucose, which decreased in the presence of sucrose (the condition required for dextran synthesis). How-ever no influence of the carbon source was detected for the adhesion ability of the other Lc. mesenteroidesstrains, which showed variable levels of binding to the enterocytes.
Article114 :Année de publication 2017
Titre Morphological study and Caracterisation of Fusarium oxysporum f.sp.albedinis. by Isozymes systems
   
Revue, référence International Journal of Scientific & Engineering Research, Volume 8, Issue 1
   
Autres auteurs Sidaoui A Karkachi N P Bertella AP Boudeffeur S P Chhiba M P Tebyaoui AP El Goumi Y Kihal M P
   
Mots Clefs Electrophoresis Fusarium oxysporium f.sp. albedinis, genetic diversity isozyme analysis
   
Résumé
   The characteristics of Fusarium oxysporium f.sp. albedinis (F.o.a) isolates were investigated using electrophoretic studies of isozymes systems (esterase and phosphatase). The morphological characteristics of the isolates were very variable to each other. The isoenzymes profiles revealed polymorphic bands or the data were subjected to analysis with the JMP method. The isolates were grouped into 2 main groups A and B, those last were divided into sub-groups. Nineteen (19) isolates creates the group A and four isolates (E1, E2, E3 and M15A) formed the group B. analysis of isozyme banding patterns were found to be a reliable marker technology, efficient and effective tools to find the genetic variability among isolates isolated in different geographical areas.Index Terms— Minimum 7 keywords are mandatory, Keywords should closely reflect the topic and should optimally characterize the paper. Use about four key words or phrases in alphabetical order, separated by commas.
Article113 :Année de publication 2016
Titre Bioethanol production from date palm fruit waste fermentation using solar energy
   
Revue, référence African Journal of Biotechnology, Vol. 15(30), pp. 1621-1627,
   
Autres auteurs Ahmed Boulal Mabrouk Kihal Cherif Khelifi Boudjemâa Benali
   
Mots Clefs Algerian Sahara, bioethanol, dates-palms waste valorization distillation, fermentation solar energy, Saccharomyces cerevisiae
   
Résumé
   Every year, more than 236,807 tons, equivalent to 30% of date-palm fruits produced in Algeria, is lost during picking, storage, and commercialization processes. Gasification of this huge biomass can generate biogas such as bioethanol, biodiesel, gasoline and other useful substances. Bioethanol is becoming the main biofuel produced by chemical synthesis or anaerobic fermentation from biomass and is significant for industrial development, investment, and use. It is eco-friendly, moderately costly and cleaner than other gasses. Actually, due to modern biotechnologies, it is possible to valorise the common date-palm waste (CDPW) by bioconversion and to commercialize them in local and international markets in the form of new products with an acceptable added value such as bioethanol. CDPW is a renewable and sustainable resource of energy that is not greatly used in industries. The date is rich in biodegradable sugars, providing bioethanol after fermentation during 72 h at 30°C in the presence of Saccharomyces cerevisiae yeast and the distillation of date’s juice obtained. In the first experience, a solar batch fermenter (SBF) of 50L capacity, and a butane gas distiller using a cocotte (cooker) of 30L capacity was designed and constructed. The bioconversion systems led to the production of 250 mL/kg of ethanol at 90° after distillation of the CDPW juice at 78°. This is in comparison to the theoretical ethanol directly produced from sugar by chemical synthesis process. The 33% efficiency that was obtained appeared satisfactory and it encouraged the great scaling development of bioethanol based on CDPW biomass and other raw materials abundant in Algeria Sahara.
Article112 :Année de publication 2016
Titre Characterization of Fusarium oxysporum isolates from tomato plants in Algeria
   
Revue, référence African Journal of Microbiology Research, Vol. 10(30), pp. 1156-1163, 14 August, 2016
   
Autres auteurs Abdesselem Si Mohammed Nisserine Hamini Kadar Mebrouk Kihal Jamal Eddine Henni José Sanchez Eduardo Gallego José Antonio Garrido
Cardena
   
Mots Clefs Fusarium oxysporum, , tomato molecular identification, pathogenicity.
   
Résumé
   Fusarium oxysporum is an ubiquitous soil-borne fungus, having a high genetic and ecological diversity with the potential to cause diseases of many crop species of economic interest. Indeed, some strains of F. oxysporum known as pathogens generate common diseases such as wilting, root and crown rot on host plants. Two formae speciales are confined to the tomato: F. oxysporum f.sp. lycopersici (FOL) causing Fusarium wilt, while F. oxysporum f.sp. radicis-lycopersici (FORL) causes Fusarium crown and root rot. The study include 27 strains isolated from the stems, crown and roots of infected tomato plants; to confirm the identity of the fungus, the isolates were identified using analysis based on morphological criteria and sequencing of the translation elongation factor 1-alpha (TEF) gene using ef1 and ef2 primers. Twenty three strains belonged to F. oxysporum, three strains to Fusarium solani, and one strain to Fusarium redolens. Tomato seedlings were tested to confirm the pathogenicity of the isolates tested. Pathogenicity test confirmed that twenty two F. oxysporum isolates were pathogenic on tomato and produced crown and root rot typical of F. oxysporum f.sp. radicis-lycopersici forma specialis, while one strain initially identified as F. oxysporum did not induce disease symptoms and is considered as non-pathogenic. Additionally, no symptoms of Fusarium wilt were observed at all; therefore no strains can be affiliated to F. oxysporum f.sp. lycopersici forma specialis.
Article111 :Année de publication 2016
Titre Effect of Cold Storage and Industrial Aromas on the Viability of Bifidobacteria in Fermented Dairy Products
   
Revue, référence World Journal of Dairy & Food Sciences, 11 (1): 18-23, 2016
   
Autres auteurs Djellid Youssef Heddadji Miloud Mahamedi Alla Eddine Kihal Mebrouk
   
Mots Clefs Bifidobacteria Milk Technological propertie Viability Cold storage Artificial aromas
   
Résumé
   Cultivation and viability of bifidobacteria in milk face several difficulties because milk is not a good medium for growth and surviving of these fastidious microorganisms. Therefore, suitable strains of Bifidobacterium should be selected basing on their technological properties. Two of the most important technological factors were examined in this study: the viability of six Bifidobacterium strains was evaluated for 21 days of cold storage in milk, as well as the effect of industrial aromas was also assessed for the same period. Very high fatalities were noted for all strains stored at 4°C, where the best viability rate was about 28%, reveling the harmful effect of this factor on the bifidobacteria’s surviving, in the other hand, difference in behavior towards artificial aromas was recorded with a best viability rate of about 70% registered for banana and 64.5% for the vanilla.
Article110 :Année de publication 2016
Titre Eliker-trimethoprim medium: A new selective tool for the isolation and growth of Lactococcus species
   
Revue, référence Advances in Environmental Biology, 10(1) :: 42-49
   
Autres auteurs Senouci Djamal Eddine Benatia Saliha Bensaid Nacera Guessas Battache Heddadji Miloud Kihal Mebrouk
   
Mots Clefs selection, , M17 medium, Elliker medium Lactococcus, growth, raw milk identification Trimethoprim Rifampicin
   
Résumé
   Lactic acid bacteria are phenotypically a very heterogeneous group. This group is characterized by the type of common criteria Gram positive, absence of catalase producing mainly lactic acid. The genus Lactococcus recently created includes non-hemolytic streptococci and homofermentative. The evaluation of exogenous Lactococci of dairy samples is frequently required for the evaluation of the milk colonization by Lactococci. In this study, different culture media, Elliker and M17 trimethoprim and M17 and Elliker rifampicin, have been tested for Lactococcus strains isolated from raw goat's milk in Oran area. A selective medium designated Elliker-trimethoprim medium (ETM) was developed for the growth of exogenous Lactococcus species. In quantitative assays, ETM showed a higher sensitivity than M17-Trimethoprim medium (M17-TM). The ETM medium allowed the selective growth of the following Lactococcus species: Lactococcus lactis (subsp. Lactis; subsp. cremoris; subsp. lactis biovar. Diacetylactis), Lactococcus plantarum and Lactococcus raffinolactis. Whereas, the specie Lactococcus piscium was reaveled only when rifapicine was used as selective agent. No Enterococcus species were observed with 0,3µg/ml of Trimethoprim or rifampicin. After several tests and comparative study, the ETM medium was chosen to follow the specific isolation and growth of Lactococcus species. Morphological, physiological and biochemical identification tests confirmed that the 40 isolates belong to the genus Lactococcus. 17 isolate (42.5%) belonging to the subspecies Lactococcus lactis subsp. cremoris were revealed on ETM. This culture medium is a new microbiological tool that can solve a problem of isolation of Lactococcus species which are much researched for food industry.
Article109 :Année de publication 2016
Titre Comparison of RAPD-PCR and PFGE analysis for the typing of Streptococcus thermophilus strains isolated from traditional Turkish yogurts
   
Revue, référence Annals of Microbiology, Volume 66, Issue 3, pp 1013–1026
   
Autres auteurs Fatima Ghazi Mebrouk Kihal Neslihan Altay
Güzin Candan Gürakan
   
Mots Clefs Streptococcus thermophilus- Typing PFGE RAPD PCRComparison
   
Résumé
   The dairy industry is constantly developing new starters with specific traits. Therefore, strains from natural sources have huge potential for exploration and characterization. The aim of this work was to identify the most efficient, useful, and reliable option for typing 55 Streptococcus thermophilus isolates from traditional Turkish yogurts and four industrial strains. Then, we statistically evaluated whether our conclusions could be extended to the true population. For this purpose, the results of the rapid amplified polymorphic DNA polymerase chain reaction (RAPD-PCR; using the OPI-02 MOD, M13, and XD9 primers) and pulsed-field gel electrophoresis (PFGE; using the SmaI and ApaI enzymes) typing methods were compared. The discriminatory power, typeability, and reproducibility were analyzed. Additionally, the congruence between typing methods was quantified using the adjusted Rand index (AR), Wallace index (W), and expected Wallace coefficient. Both methods revealed high genetic diversity of the S. thermophilus strains, even in the same yogurt sample. The numerical combination of results for these primers or restriction enzymes increased the congruence between the methods and provided more complete information on the strains. The comparison of these two options showed that using SmaI with ApaI was more advisable and useful than using OPI-02 MOD with M13. Additionally, the first combination represented the best tool to discriminate S. thermophilus strains (Simpson’s index of diversity [DI] of 0.999 [0.997–1.000]). This finding was statistically supported. The RAPD (OPI-02 MOD) typing result showed an ability to confidently predict the PFGE (SmaI, ApaI) type (AR = 0.782 [0.618–0.949], W = 0.946 [0.865–1.000]). This result had some statistical support and might represent an important application in the dairy industry for screening strains.
Article108 :Année de publication 2016
Titre Antimicrobial Susceptibility Profiling of Staphylococcus aureus Isolates from Milk
   
Revue, référence British Microbiology Research Journal, 13(3): 1-7, 2016, Article no.BMRJ.24064
   
Autres auteurs W. Chaalal H. Aggad K. Zidane N. Saidi M. Kihal
   
Mots Clefs Staphylococcus aureus milk antimicrobial resistance MRSA
   
Résumé
   Aims: This study investigated the occurrence of Staphylococcus aureus in milk and its sensitivity to twenty antibiotics. Study Design: The research was laboratory-based investigation. Place and Duration of the Study: The study was carried out at the Laboratory of Hygiene and animal pathology, University of Tiaret, Algeria, between September 2012 and May 2013. Methodology: Thirty eight milk specimens were collected from cattle and examined to estimate the prevalence of Staphylococcus aureus. The sensitivity of the isolates to twenty (20) antibiotics was evaluated and the presence of methicillin resistant Staphylococcus aureus (MRSA) was also determined. S. aureus was characterized using standard microbiological methods and confirmation was done using the API Staph Identification System. Antibiotic sensitivity of isolates was evaluated by means of agar diffusion technique while the minimum inhibitory concentration (MIC) was established using broth dilution technique for oxacillin, E-test for tetracyclin, and chloramphenicol respectively. Results: 55.26% of analyzed samples were contaminated with S. aureus. 100% of Methicillin Sensitive S. aureus were resistant to nalidixic acid, 70% to bacitracin, 65% to spiramycin, and 45% to penicillin and fosfomycin. There was no resistance to vancomycin, chloramphenicol, gentamicin and pristamycin among isolates. A total of 76% of the isolated strains were found to be resistant to at least 4 antibiotics. One Methicillin Resistant S. aureus strain (4.76%) was detected and showed multiple drug resistance. This resistance was crossed with all beta lactams and its resistance profile to macrolides was constitutive (MLSB const) while aminoglycosides phenotype was ANT (4’) (4’’). Conclusion: A high prevalence of S. aureus with multiple drug resistance was established. Improved food safety measures are thus necessary to prevent transmission and spread of antimicrobial resistance by these pathogens.
Article107 :Année de publication 2016
Titre Immobilization Of Lactic Acid Bacteria On Bentonite Clay Particles of Maghnia Region West-Algerian
   
Revue, référence Advances in Environmental Biology, , 10(10) 6, Pages: 33-39 ( http://www.aensiweb.com/AEB/)
   
Autres auteurs Moulay Meriem

j and. Hadj Said Aissa Hocine Lared Kihal Mebrouk
   
Mots Clefs Clay of Maghnia Lactic acid bacteria, Streptococcus thermophilu Lactococcus lactis subsp. lactis biovar. diacetylactis Lactobacillus fermentum Bio sorbent
   
Résumé
   Microorganisms may attach to the solid surfaces by their bio adhesive properties in order to form a biofilm, which can be used in several areas, including those in the food and the treatment of water. A re-identification of three strains of lactic acid bacteria (Lactobacillus fermentum, Streptococcus thermophilus and Lactococcus lactis subsp. lactis biovar. diacetylactis) have been carried out by its phenotypes characterizations (Macroscopic on culture media MRS and M17 (liquid and solid), Microscopic after Gram stain, biochemical whose catalase test and fermentation Type before testing their capital assets on clay particles in suspension, thus forming the complex bio sorbents. After centrifuging the suspension, we observed a good fixation of these particles, in particular the Lactococcus lactis subsp. lactis biovar. diacetylactis and Streptococcus thermophilus, respectively with a number of attached cells of 72x107 and 70x107 cfu/ml, and this after 4 hours of incubation at 30 and 37°C, whereas the Lactobacillus fermentum was less with only 40x106 cfu/ml. We have also observed an obvious increase in the growth of the number of cells Lactococcus lactis subsp. lactis biovar. diacetylactis in the presence of clay particles in suspension, from an initial number of 40x106 cfu/ml to 85x107 cfu/ml after 24 hours of incubation, this is probably due to the catalytic role played by the clay particles in suspension in their multiplication.
Article106 :Année de publication 2015
Titre Influence of two substrates (casein and glucose) on mycelia growth and dosage of proteolytic activity of Fusarium oxysporum f. sp. albedinis (Foa)
   
Revue, référence International Journal of Biosciences, Vol. 6, No. 5, p. 115-125,
   
Autres auteurs Wassim Yezli
Nebia Zebboudj
Nour Eddine Karkachi
Mebrouk Kihal
Jamal Eddine Henni
   
Mots Clefs casein, glucose, mycelia, proteolytic, Fusarium oxysporum, growth
   
Résumé
   Résumé (Abstract) The influence of the two substrates tested on 18 isolates of Fusarium oxysporum f. sp. albedinis (Foa) was studied in order to compare the fungus behaviour on Casein and Glucose. It has shown that there is a mycelia growth of 63.63 % using casein, compared to 36.36 % using the Glucose. It has also been observed that the isolate "Foa Mtl 8" has shown a superior growth compared with the other isolates on liquid and solid casein medium, with a weight of 3 g, and it has completely covered the Petri dishes in first (in 6 days). On the same isolate, the detection of proteolytic activity by measuring the clarification diameters was most significant (4.2 cm diameter). On these criteria we had been based in order to select the isolate “Foa Mtl 8” for the determinati on of proteolytic activity. The colorimetric protein assay was performed by the method of Folin and Ciocalteu at different initial pH (4, 6 and 8). It had also shown that the isolate “Foa Mtl 8” produce a protease in pH range of 7.1 to 7.8.
Article105 :Année de publication 2015
Titre Resistance at Low Ph Values and Bile Tolerance for Selection of Bifidobacterium Strains Isolated from New Born Feces as Potential Probiotic
   
Revue, référence Advances in Environmental Biology, 9(14) July 2015, Pages: 122-128
   
Autres auteurs Kheira Mehdi
Miloud Hadadji
Bachir Benkaddour
Betache Guessas
Mebrouk Kihal
   
Mots Clefs bifidobacteria, bifidobacterium longum, probiotic, lactic acid, acetic acid
   
Résumé
   With the growing interest in health consciousness and idea of health promotion without associated health risks, the concept of probiotic foods has attracted much attention. Microbe such as Bifidobacterium is the most common organisms used as probiotic. In this study, 09 strains of bifidobacteria were isolated from new born feces, and identified using the API 20 A kits. The main phenotypic characteristic of Bifidobacterium is the production of lactic and acetic acids with a theoretical molar ratio (acetic:lactic) of 3:2, this organic acids were assessed with HPLC system. The carbohydrate fermentation permitted us to identifier 03 species b.longum (04 strains), b.breve (03starins) and b.bifidum (02 strains). This present work investigated the survival of Bifidobacteria in the gastrointestinal transit under in vitro conditions; tolerate of low ph of the stomach and survive under 0,3, 0,5, and 1% of bile concentration. Results showed a large variation in the viability of all strains in both, low ph and high concentration of bile. The only strain how present a highest rate of surviving is b.longum which has been selected for further investigation in vivo.
Article104 :Année de publication 2015
Titre Antagonistic effect of Trichoderma harzianum against Phytophthora infestans in the North-west of Algeria
   
Revue, référence International Journal of Agronomy and Agricultural Research (IJAAR), Vol. 6, No. 4, p. 44-53, 2015
   
Autres auteurs Kerroum Fatima
Karkachi Noureddine
Jamal Edinne Henni
Kihal Mabrouk
   
Mots Clefs Phytophthora infestans, Trichoderma harzianum, Biological Control, Tomato, Potato
   
Résumé
   The oomycete, Phytophthora infestans is considered one of the most important pathogens of potatoes and tomatoes worldwide. A total of 38 Phytophthora infestans isolates were obtained from leaves, tubers and stems of infected crops of potato and tomato in different regions of the North West of Algeria in 2010, 2011 and 2012. Based on morphological and physiological characteristics, they were tested for the virulence test on potatoes tubers and tomatoes leave then, for a biological control by using Trichoderma spp. as antagonistic agent. Trichoderma species are among important antagonists of plant pathogenic fungi. The main purpose of this study was to evaluate the biocontrol potential of native Trichoderma harzianum. Their antagonistic activities including competition and colonization against Phytophthora infestans with an inhibition rate of 86%
Article103 :Année de publication 2015
Titre Detection of paratuberculosis using histopathology, immunohistochemistry, and ELISA in West Algeria
   
Revue, référence Comp Clin Pathol, DOI 10.1007/s00580-015-2126-8
   
Autres auteurs H. Hemida
M. Kihal
   
Mots Clefs Paratuberculosis. Mycobacterium avium subsp. paratuberculosis. Immunohistochemistry. Histopathology. ELISA
   
Résumé
   Paratuberculosis (Ptb), caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic enteritis that affects many ruminants and other wild animals worldwide. Although the prevalence of infection is unknown in nearly all countries, many studies report that paratuberculosis is the greatest economic problem in animal health. Here, we report the occurrence of paratuberculosis in herds of cattle in the Tiaret region of Algeria, using different diagnostic procedures. Tiaret and Sougueur abattoirs were being selected representing the main parts of the province. Four hundred intestines were examined from apparently healthy cattle that were slaughtered at Tiaret city (300) and Sougueur (100) slaughterhouses. Occurrence of subclinical paratuberculosis in cattle was 54, 51, 8, and 7.5 % using histopathology, IHC, ELISA, and ZN techniques, respectively. Histopathology is reported to be a better diagnostic tool mainly when complemented with immunohistochemistry
Article102 :Année de publication 2015
Titre Purification and Molecular Characterization of Two-Antimicrobial Peptides Produced by Lactobacillus plantarum DU10
   
Revue, référence International Journal of Biological Chemistry, 9 (2): 46-58, DOI: 10.3923/ijbc.2015.46.58
   
Autres auteurs Zergui Amina
Saidi Noureddine
Arul Venkatesan
Bessaiah Hicham
Benmalek Yamina
Bouayad Leila
Kihal Mebrouk
   
Mots Clefs Lactobacillus plantarum, probiotic, bacteriocin, purification, antimicrobial peptide
   
Résumé
   The aim of this study was the partial characterization of plantaricin MZ, a two-component antimicrobial peptides produced by the putative probiotic bacteria Lactobacillus plantarum DU10, which was isolated from Algerian raw camel milk. This biomolecule was secreted in the supernatant of a L. plantarum DU10 culture and showed diverse spectrum of antimicrobial activities against several pathogenic bacteria. The activity as determined by the proteolytic action of trypsin, pepsin and proteinase K, plantaricin MZ was maintained even after a treatment at 121°C for 15°C and a pH range from 2-10. This putative probiotic strain was found to produce antimicrobial substances proteinaceous in nature. Molecular mass determination was estimated with tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis, then confirmed by mass-assisted laser desorption ionization time of flight mass spectrometry. The producing strain and its antimicrobial peptides may find an application as a bio-preservative agent.
Article101 :Année de publication 2015
Titre Control of E. coli and spoilage microorganisms in tomato sauce and paste using a synergistic antimicrobial formula
   
Revue, référence Journal of Chemical and Pharmaceutical Research, 7(5):1352-1360 http://www.jocpr.com
   
Autres auteurs Djadouni Fatima
Kihal Mebrouk
Heddadji Miloud
   
Mots Clefs Biopreservation, tomato, Solanum lycopersicum, bacteriocins, lactic acid bacteria
   
Résumé
   The aim of this study was to assess the potential of lactic acid bacteria to inhibit the outgrowth of some common food-spoiling fungi and bacteria. 40 lactic acid bacteria (LAB) and 20 Bacillus strains isolated from the fermented tomato (Solanum lycopersicum) for their capacity to produce antimicrobial activities against several bacteria and fungi. The strain designed LBc03 has been selected for advanced studies. The supernatant culture of this strain inhibits the growth of Escherichia coli, Staphylococcus aureus and Aspergilus sp. Based on the cultural, morphological, physiological and biochemical characteristics, LBc03 was identified as Leuconeustoc spp. Its antimicrobial compound was determined as a proteinaceous substance, but it is possible that the bacteriocin may also be bound to other molecules like a lipid or a carbohydrate moiety. Metabolite extracts from selected LAB were more effective in preserving tomato paste and sauce stored at 4°C against spoilage bacteria like E. coli and the application of bio-preservative should be encouraged in food processing industries.
Article100 :Année de publication 2015
Titre Biopreservation of tomato paste and sauce with Leuconostoc spp. metabolites
   
Revue, référence African Journal of Food Science, 9(6) pp. 359-366, http://www.academicjournals.org/AJFS
   
Autres auteurs Djadouni Fatima
Kihal Mebrouk
Heddadji Miloud
   
Mots Clefs Biopreservation, tomato (Solanum, lycopersicum), bacteriocins, lactic acid bacteria
   
Résumé
   The aim of this study was to evaluate 40 lactic acid bacteria (LAB) and 20 Bacillus strains isolated from the fermented tomato (Solanum lycopersicum) for their capacity to produce antimicrobial activities against several bacteria and fungi. The strain designed LBc03 has been selected for advanced studies. The supernatant culture of this strain inhibits the growth of Escherichia coli, Staphylococcus aureus and Aspergilus sp. Based on the cultural, morphological, physiological and biochemical characteristics, LBc03 was identified as Leuconeustoc spp. Its antimicrobial compound was determined as a proteinaceous substance, but it is possible that the bacteriocin may also be bound to other molecules like a lipid or a carbohydrate moiety. Metabolite extracts from selected LAB were more effective in preserving tomato paste and sauce stored at 4°C against spoilage bacteria like E. coli and the application of bio-preservative should be encouraged in food processing industries.
Article 99 :Année de publication 2015
Titre Purification of bacteriocin BacPC from Pidiococcus pentocaseus sp. suitable for foods preservation
   
Revue, référence International Journal of ChemTech Research, Vol.8, No.1, pp 15-21,
   
Autres auteurs Djadouni Fatima
Heddadji Miloud
Kihal Mebrouk
   
Mots Clefs Pediococcus pentosaceus sp, Staphylococcus aureus, bacteriocins, purification, MIC
   
Résumé
   Bacteriocins were metabolized products of food – grad, microorganisms and some of which were used as food preservative to improve the food safety. Bacteriocin producing Pediococcus pentosaceus (BacPC) was protein in nature with antimicrobial effects on some clinically important food borne pathogens. This strain was isolated from the milk products samples and they were cultivated on MRS agar and identified using biochemical methods. The purification technologies, the activity determination, and the characteristics of the bacteriocin were also explained. The results suggested that the protein bands were observed with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass 19 KDa (BacPC). The mode of action of the partial purified Bacteriocin was identified as bactericidal against Staphylococcus aureus. The minimal inhibitory concentration (MIC) of bactériocin (BacPC) for the indicator organism Staphylococcus aureus was 6 mg.ml-1. Results presented here support the idea that the bacteriocin may propose some industrial advantage that renders it as a good natural food biopreservative candidate.
Article 98 :Année de publication 2015
Titre Metagenomic analysis of the bacterial microbiota linked to the traditional Algerian date product « Btana »
   
Revue, référence Ann Microbiol, doi:10.1007/s13213-015-1084-3
   
Autres auteurs Abdelkader Abekhti
Bernard Taminiau
Mabrouk Kihal
Georges Daube
   
Mots Clefs Pyrosequencing, Bacterial diversity, Traditional food ., Dates, Phoenix dactylifera L, Btana
   
Résumé
   In this study, using high throughput Bpyrosequencing, we highlighted the bacterial diversity of the traditional Algerian date product BBtana^ that is produced in southern Algeria using both direct (DBM) and indirect (UBM) methods. Metagenomic analysis yielded a total of 103,379 reads, with a 606 total operational taxonomic units (OTUs) detected. Firmicutes represented 84.79 % of the total pyrosequencing reads. Phylogenetic analysis revealed that the Bacillales represented 90.20 % ± 15.12 % of the total reads. Among the phylotypes detected, Bacillus was the dominant genus (39.53 %). While Bacillus megaterium was shared among all of the samples, its distribution varied widely. Non-metric multidimensional scaling (nMDS) analysis showed that UBM samples clustered together, and three main OTUs were found in these UBM samples: Paenibacillus polymyxa, Paenibacillus xylanexedens, and Planomicrobium JN082684. Correlation analysis showed no association between parameters of the samples (age, pH, water activity) and the specific microbiota.
Article 97 :Année de publication 2015
Titre Isolation and physicochemical test studies of Ascochyta pisi
   
Revue, référence International Journal of Biosciences, Vol. 6, No. 1, p. 9-19, 2015
   
Autres auteurs R. Terbeche
N.E. Karkachi1
S. Gharbi2
M. Kihal1
J.E. Henni1
   
Mots Clefs Pisum sativum, Ascocgyta pisi, physicochemical, factoring, carbon source, nitrogen source
   
Résumé
   Anthracnose caused by pea Ascocgyta pisi is one of the most serious diseases affecting crops of peas (Pisum sativum L.) worldwide. In Algeria, more precisely in the north-west, quantitative data on the incidence of the disease is not available; it proved essential as a first step to isolate the pathogen and study in vitro the physicochemical factors affecting its growth. 10 strains were selected from 32 isolates from infected plants. The results indicate that the degree of the temperature of incubation affects the mycelia growth, at 4°C, mycelia growth was slowed and 37 °C inhibits the mycelia growth. We recorder that the optimum growth is between 22 and 25°C. Relative humidity affects the mycelia growth of the strains, the rate ≤ 50%, it is low at 74%, it is average, and other rate ≥ 80%, and it is optimal.. The usual media used (PDA, Malt, Mathur) give a good mycelia growth of the strains, but some have very low growth in the middle of pea; Overall, the usual empirical media used do not affect the mycelia growth of the strains. All strains assimilate the different carbon sources, however, is optimal mycelia growth on the (lactose, maltose and starch), the average (glucose, fructose), and weak on the cellulose. Mycelia growth is optimal on low nitrogen sources NaNO3, KNO3 and asparagine, on Valine, Leucine and L-arginine media, and slowed on the environment (NH4)2SO4 the nature of the nitrogen source influences on the growth of the strains.
Article 96 :Année de publication 2015
Titre Bacteriological quality of drinking water originated from groundwater in Béni-Abbés area (South West Algeria)
   
Revue, référence African Journal of Agricultural Research, Vol. 10(53), pp. 4844-4849,
   
Autres auteurs BENGARNIA Benmerine HADADJI Miloud RAMDANI Mohammed KIHAL Mebrouk
   
Mots Clefs Bacteria Béni-Abbès drinking wate groundwater quality, indicators microbial analysis Escherichia coli.
   
Résumé
   The purpose of the present study was to examine the bacteriological quality of the drinking water samples to evaluate the results with the Algeria and international standards for drinking water quality, as well as the identification of the dominant microflora. The water samples (groundwater) are collected monthly from six sites in Béni-Abbés district. Most of the samples have shown the occurrence of Escherichia coli, in addition to Salmonella sp and Clostridium sp. Out of 300 water samples collected, 126 (42%) were contaminated with either one or more than one type of microorganisms: Enterobacter gergoviae detected in 26 samples (11.06%), E. coli in 63 (22.98%), Citrobacter freundii in 16 (6.81%), Vibrio vulnificus in 2 (0.85%), Pseudomonas aeruginosa in 19 samples (8.09%) and Serratia liquefaciens in 22 (9.36%). Clostridium was the common cause of contamination, about 45 (16.60%), and Salmonella sp was detected in 66 (24.25%), Salmonella typhi in 44 samples (18.72%) and Salmonella paratyphi in 13 samples (5.53%). The findings of this study highlight the need for a more stringent self-control of drinking water. In addition, a more systematic surveillance by the official authorities of drinking water is also necessary
Article 95 :Année de publication 2015
Titre Study of Toxic Profile of Staphylococcus Aureus Isolated From Raw Milk Samples of Mastitis SOR Consumption in Oran Area, Algeria
   
Revue, référence International Journal of Scientific Research in Science and Technology, Volume 1 | Issue 6 | : 4-6
   
Autres auteurs Nadjia BENHAMED Fatma BELHOUCINE Mustapha MOHAMED BENKADA Philippe GAUTIER Amel ALIOU P
Y DONNIO Mebrouk KIHAL
   
Mots Clefs milk, mastitis Staphylococcus aureus Toxic profile pvl TSST-1, sea, seb.
   
Résumé
   Mastitis S. aureus is considered one of the major diseases in dairy cattle. The presence of S. aureus causes damage to the mammary gland tissue that will impact milk production in both volume and quality. The many virulence factors such as toxins and proteins or surface polysaccharides are involved in the pathology. This study determines the toxic profile of S. aureus strains isolated from raw milk samples from bovine mastitis diagnosed in dairy farms in Oran. After identification by MALDI-TOF, spa typing and MLST have identified the phylogenetic position of the strains. Search for virulence factors is performed by real-time PCR TaqMan type, to detect manipulation in one of the four genes of virulence factors of S. aureus: tst for TSST-1, pvl leucocidin for ED, sea for enterotoxin A, seb for enterotoxin B, in this study sought. Toxins research shows that only a few strains have proven carriers of different virulence genes including the gene encoding the pvl Panton-Valentine leukocidin. Other strains were positive for the presence of tst gene encoding the toxin of toxic shock syndrome (TSST-1), 100% of the Staphylococcus aureus isolates identified were sensitive to methicillin. In conclusion, the results obtained in this work have determined the phylogenetic profile, toxic and sensitivity profile of the méticiline strains studied. Stem found produisantes as the Panton-Valentine leukocidin are sensitive to méticilline. Cette study is the first molecular characterization study of animal strains of S. aureus isolated in the region of Oran.
Article 94 :Année de publication 2014
Titre Morphological, physiological and pathogenic variability of small-spore Alternaria causing leaf blight of Solanaceae in Algeria
   
Revue, référence African Journal of Microbiology Research, 8(37), pp. 3422-3434
   
Autres auteurs Bessadat N
Simoneau P
Benichou S
Setti B
Kihal M
Henni Djamel Eddine
   
Mots Clefs Epidemiology, Solanaceae, small-spore, Alternaria species, pathogenic variability, cultural characters, physiological diversity
   
Résumé
   Due to premature defoliation, early blight epidemics can cause major yield losses. Large-spore Alternaria species such as A. solani and A. tomatophila have long been recognized as important pathogens responsible for such blight disease in the family Solanaceae and thus represent a serious risk for crop production. Small-spore Alternaria species have also been frequently isolated from plant samples with typical blight symptoms but their incidence as primary pathogens is often controversial. In order to study the diversity of small-spore Alternaria species, 32 isolates were selected from a larger collection of 130 isolates from infected leaves, fruits and stems of tomato from various growing regions of North-West Algeria. Morphological characterization under standard conditions and polymerase chain reaction (PCR) analyses using specific primers to amplify a part of the ITS regions and the 5.8S gene were conducted to confirm their identification as members of the alternata section. They were then examined according to morphological characteristics of conidia and sporulation patterns on potato carrot agar (PCA) and were segregated into three morphological species: A. alternata, A. tenuissima and A. arborescens. Colony type, substrate colour, margin, zonation, pigmentation, colony diameter and conidia production were studied on potato sucrose agar (PSA). Physiological parameters and nutritional requirements of the isolates were also assessed and a data matrix based on cluster analysis and Euclidean distance was constructed. Results of pathogenicity test on tomato showed obvious diversity among the isolates and they could be separated into two groups based on their virulence. The dendrogram based on the influence of cultural, nutritional and physiological characters suggests moderate heterogeneity within the populations of A. alternata and A. tenuissima. The small-spore species formed five clusters that fundamentally paralleled the morphological groupings. However, the results provided no evidence for geographical and pathogenicity clustering of isolates.
Article 93 :Année de publication 2014
Titre Aggressiveness and morphological variability of small spore Alternaria species isolated from Algeria
   
Revue, référence Journal of Experimental Biology and Agricultural Sciences, 2(2S)
   
Autres auteurs Bessadat Nabahat
Benichou Soumaya
Kihal Mebrouk
Henni Djamel Eddine
   
Mots Clefs Tomato, Small spore, Alternaria, Identification, Bioassay, Blight disease, Pathogenicity
   
Résumé
    Natural epidemics of blight disease are strongly influenced by environmental conditions, even though several diseases appears every year in north western of Algeria which can cause complete loss of the crop when the infection is severe. The fungi which are frequently associated with leaf blight, stem blight and apical fruit rot of Solanaceae family are Alternaria arborescens, A. alternata and A. tenuissima. These fungi were identified on morphological characteristics. For that, eighty one small spore strains belonging to the A. alternata section were assessed for disease symptoms, percent disease incidence (PDI) and area under the diseases progress curve (AUDPC) on three different tomato cultivars. Among the tested fungal isolates, it revealed that PDI of the isolates were changed according to the cultivar and the highest blight disease incidence was found in A. tenuissima strain 164 (96% in Saint Pierre, 59% in Cherry tomato and 69% in Rio Grande), it was followed be A. alternata strain 156 (90% in Saint Pierre, 82% in cherry tomato and 46% in Rio Grande) and A. arborescens strain 65 (49% in Saint Pierre, 85% in cherry tomato and 63,15% in Rio Grande). According to the aggressiveness component of the isolates, the classical behavior of the cultivars was confirmed, and Saint Pierre and Rio Grande cultivars were found susceptible with a slightly higher mean of AUDPC (413.72 and 390.48 respectively )but Cherry tomato cultivar was found to be resistant with the lowest AUDPC mean 227.18 ± 166.10. Based upon results of present study, it was concluded that a complex of small spore Alternaria species and isolates found on Solanaceae lesions are not equally pathogenic but majority of A. tenuissima isolates are not the part of this complex.
Article 92 :Année de publication 2014
Titre In vitro effect of two fungicides on pathogenic fungi causing root rot on tomato in Algeria
   
Revue, référence African Journal of Agricultural Research, 9(33), pp. 2584-2587
   
Autres auteurs Hamini
Kadar Nisserine
Benaouili Hajira
Benichou Soumaya
Setti Benali
Kihal M
Henni Djamel Eddine
   
Mots Clefs Fusarium species, mycelium growth, inhibition, trifidan, antracol, tomato diseases, control, fungicides
   
Résumé
    Tomato (Lycopersicon esculentum) is one of the most widely grown vegetables in the world. There is a growing concern in recent years, both in developed and developing countries, about the use of hazardous fungicides for controlling plant diseases. The in vitro effect of two fungicides commonly used in Algeria namely Trifidan and Antracol (propineb) on the growth of three pathogenic fungi of tomato, Fusarium oxysporum f.sp. radicis-lycopersici, Fusarium commune and Fusarium redolens was investigated to determine the effectiveness of the fungicides in reducing fungi growth. Each fungicide was assayed at 0, 100, 200, 400 and 500 mg L-1 rate in Potato Dextrose Broth (PDB) and incubated at 28°C for seven days. Data were collected on the mycelia weights of the fungi under each treatment. Mycelia weights of the three fungi were significantly reduced at 100 mg L-1 by the two fungicides under antracol treatment F. redolens and F. commune and a significant reduction was observed at 400 and 500 mgL-1. Application of Trifidan, significantly decrease in mycelia weight of F. oxysporum f. sp. radicis-lycopersici and F. commune irrespective of the rate applied. F. redolens was completely inhibited by Trifidan, and its inhibition started at 100 mg L-1.
Article 91 :Année de publication 2014
Titre Isolation and physiochemical studies of Fusarium oxysporum f.sp radicis lycopersici
   
Revue, référence Advances in Environmental Biology, 8(10), 36-49, 2014
   
Autres auteurs Benaouali H
Hamini
Kadar N
Benichou Soumaya
Kihal M
Henni Djamel Eddine
   
Mots Clefs Fusarium oxysporum, pathogenicity, test, various media, pH, light, humidity, Carbone, Nitrogen
   
Résumé
    The study of 27 isolates of F.oxysporum from different regions of western Algeria(Oran, Mostaganem, Chleff) Allowed on the basis of their macroscopic appearance to differentiate four morphotypes (downy, cottony, ras mucous and senescent) with different pigmentation (white, yellow, pink and purple).The pathogenicity test confirmed the presence of Fusarium oxysporum f.sp radicis lycopersici in the western region of Algeria. Of the 27 strains studied 22 have shown signs of crown and root rot, with very high degrees of virulence compared to the references strains and 5 isolates were not pathogen. For a better understanding of the ecology of these isolates a study of the influence of different physicochemical conditions were realized. The results showed a better growth of the isolates on PDA medium with an important pigmentation, mycelia growth of different isolates is quiet faster in the darkness than in light and the optimum pH for growth was 6 and 7 with a rate of humidity ranging from 74 to 80% and an optimal growth temperature of 23°C and 28°C.
Article 90 :Année de publication 2014
Titre Antagonist effect of nonpathogenic Fusarium oxysporum strain Fo47 on Fusarium oxysporum f. sp. radicis-lycopersici the cause of fusarium crown and root rot in tomato cultivation
   
Revue, référence Advances in Environmental Biology, 8(10), 50-56, 2014
   
Autres auteurs Hamini
Kadar Nisserine
Si Mohamed Abdeslem
Benaouali Hadjira
Benichou Soumaya
Kihal Mebrouk
Henni Djamel Eddine
   
Mots Clefs Fusarium oxysporum f.sp. radicis-lycopersici, tomato, root rot and crown, disease incidence, biocontrol, nonpathogenic Fo47
   
Résumé
   Root rot and crown rot caused by Fusarium oxysporum f.sp. radicis-lycopersici (Forl) is one of the most serious and complex wilt root rot diseases affecting crops of tomato (Solanum lycopersicum L.) over the world. In this study, the antagonist effect of nonpathogenic Fusarium oxysporum strain Fo47 on the pathogen Forl was evaluated in vivo, in growth chamber by using two bioessays, in which, the investigated mechanisms involved the direct antagonism, including a competition for nutriments and root colonization, using a temporal separation of the antagonist ant the pathogen on the one hand and inducing systemic resistance to the tomato plant, using a physically separation of the antagonist and the pathogen to the same plant root level with a split-root method on the other. Results obtained from the two bioassays showed that the nonpathogenic Fo47 have significantly reduced the incidence of the disease while being separated in time or space of the pathogenic strain, especially when they were applied a week before it. These results have important practical implications for biocontrol of tomato diseases under commercial conditions.
Article 89 :Année de publication 2014
Titre Genomic and proteomic characterization of bacteriocin-producing Leuconostoc mesenteroides strains isolated from Algerian raw camel milk.
   
Revue, référence Biomed Research international, Biomed Res Int. 2014; 2014: 853238. www.hindawi.com/journals/bmri/aip/853238
   
Autres auteurs Zineb Benmechernene1 Inmaculada Fern?ndez
No2 Marcos Quintela2 Karola B?hme2 Mebrouk Kihal1 Pilar Calo
Mata2 and Jorge Barros
Vel?zquez2
   
Mots Clefs Date Fruit, Storage, Microflora, Quality, Indicator, Date Palm, Btana
   
Résumé
Article 88 :Année de publication 2014
Titre Purification of bacteriocin BacPC from Pidiococcus pentocaseus sp. suitable for foods preservation
   
Revue, référence Journal of Food Science and Engineering, 06 ;07/N2014
   
Autres auteurs Djadouni Fatima
Heddadji Miloud Kihal Mebrouk
   
Mots Clefs Pediococcus pentosaceus sp., Staphylococcus aureus, bacteriocins, purification, MIC.
   
Résumé
   Bacteriocins were metabolized products of food – grad, microorganisms and some of which were used as food preservative to improve the food safety. Bacteriocin producing Pediococcus pentosaceus (BacPC) was protein in nature with antimicrobial effects on some clinically important food borne pathogens. This strain was isolated from the milk products samples and they were cultivated on MRS agar and identified using biochemical methods. The purification technologies, the activity determination, and the characteristics of the bacteriocin were also explained. The results suggested that the protein bands were observed with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass 19 KDa (BacPC). The mode of action of the partial purified Bacteriocin was identified as bactericidal against Staphylococcus aureus. The minimal inhibitory concentration (MIC) of bactériocin (BacPC) for the indicator organism S. aureus was 6 mg.ml-1. Results presented here support the idea that the bacteriocin may propose some industrial advantage that renders it as a good natural food biopreservative candidate.
Article 87 :Année de publication 2014
Titre Isolation and identification of dominant osmophilic Leuconostoc strains from traditional date product “Btana”
   
Revue, référence International Food Research Journal, 21(4): 1297-1304 http://www.ifrj.upm.edu.my
   
Autres auteurs Abekhti
A.
Daube G.
Kihal
M
   
Mots Clefs Date, Btana, Traditional food, Osmophilic flora, Leuconostoc, Antibacterial activity
   
Résumé
   The current study aimed to isolate and identify dominant osmophilic bacteria associated with a traditional date product named “Btana”, produced in south region of Maghreb countries. Samples were randomly collected after two month of storage from tow villages (Mtarfat and Abani) in the Algerian southern department “Adrar”. A high osmotic pressure medium (MSE) was used for isolation of osmophilic bacteria, which were purified and examined for macroscopic and microscopic shape, Gram stain, catatalse, oxydase, acetoine and ADH production, reduction of nitrate, and motility. Isolates were then subculture on MRS medium for production of dextran, gas from glucose, growth in the presence of NaCl (3, 6.5 %) and sucrose (10, 20, 30, 40, and 50 %), pH tolerance (4.8, 6.5), growth temperature (10, 37, and 45°C) and thermo resistance (55°C for 15 min), enzymatic activity (proteolytic, lipolytic, hemolysis). Isolates were identified to specie’s level by sugar fermentation. Their growth and acidification kinetic were also studied. Results identified two species of Leuconostoc; Leuconostoc mesenteroides subsp. mesenteroides and Leuconostoc mesenteroides subsp. dextranicum. They show a high antibacterial activity against four indicator bacteria; Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.
Article 86 :Année de publication 2014
Titre Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolatedfrom Frozen Bovine Meat in Algeria
   
Revue, référence Advances in Environmental Biology, Advances in Environmental Biology, 8(1), Pages: 6-13 h ttp://www.aensiweb.com/aeb.html
   
Autres auteurs Barka Mohammed Salih
Kihal Mebrouk
Benyoub Nour
Eddine
Henni Jamal
Eddine
Boudilmi Benabdallah
   
Mots Clefs Escherichia coli O157:H, stx1, stx2, eae c, ehxA, PCR, frozen bovine meat, Algeria
   
Résumé
   In this study the presence of E.coli O157:H7 was investigated in 756 samples of frozen bovine meat imported from different countries using the standard method of culture and serotyping technique. The pathogen was detected in five (0.66 %) samples. All isolates were further characterized by PCR and serotyping. PCR showed that 1 isolate carried stx1 genes, 5 possessed stx2 genes and 1 both stx1 and stx2. Enterohemolysin (ehxA) and intimin (eae c) virulence genes were detected in 5 isolates and in 4 of the isolates respectively. The interaction with lactic acid bacteria revealed that all E.coli O157:H7 isolates were inhibited. Then all the isolates were tested using the method of KIRBY and BAUER according to the nccls standards to view resistance to antibiotics and was considered sensitive to the sixteen antimicrobial agents tested
Article 85 :Année de publication 2014
Titre Isolation, pathogenicity test and physicochemical studies of Fusarium oxysporum f.sp radicis lycopersici
   
Revue, référence Advances in Environmental Biology, Journal home page: http://www.aensiweb.com/aeb.html Advances in Environmental Biology, 8(10) June 2014, Pages: 36-49
   
Autres auteurs H. Benaouali
N. Hamini
Kadar
A. Bouras
S.L. Benichou
M. Kihal
J.
E. Henni
   
Mots Clefs Fusarium oxysporum, pathogenicity test, various media, pH, temperature, light, humidity, Carbone, Nitroge
   
Résumé
   The study of 27 isolates of F.oxysporum from different regions of western Algeria(Oran, Mostaganem, Chleff) Allowed on the basis of their macroscopic appearance to differentiate four morphotypes (downy, cottony, ras mucous and senescent) with different pigmentation (white, yellow, pink and purple).The pathogenicity test confirmed the presence of Fusarium oxysporum f.sp radicis lycopersici in the western region of Algeria. Of the 27 strains studied 22 have shown signs of crown and root rot, with very high degrees of virulence compared to the references strains and 5 isolates were not pathogen. For a better understanding of the ecology of these isolates a study of the influence of different physicochemical conditions were realized. The results showed a better growth of the isolates on PDA medium with an important pigmentation, mycelia growth of different isolates is quiet faster in the darkness than in light and the optimum pH for growth was 6 and 7 with a rate of humidity ranging from 74 to 80% and an optimal growth temperature of 23°C and 28°C.
Article 84 :Année de publication 2014
Titre Prevalence and Antimicrobial Resistance of Methicillin-Resistant Staphylococcus aureus Isolated from Raw Meat and Bovine Milk in Algeria
   
Revue, référence Advances in Environmental Biology, 8(1) January 2014, Pages: 1-5, Advances in Environmental Biology, 8(1) January 2014, Pages: 1-5 http://www.aensiweb.com/aeb.html
   
Autres auteurs Chaalal
W.
Aggad
H.
Saidi
N.
Kihal
M.
   
Mots Clefs MRSA, Food, Prevalence, Biotyping, Antibiotic susceptibility, Algeria.
   
Résumé
   Recently, reports of methicillin-resistant Staphylococcus aureus (MRSA) from several foods have become more frequent. The aim of this study was to estimate the prevalence of MRSA from 108 foodstuff samples of animal origin in Algeria. The strains were characterized by studying their resistance properties against several antibiotics; production of ß- lactamase by Clover Leaf technique; their ecological origin was determined by biotyping. Of the 57 analyzed S. aureus strains, two (3.5%) were MRSA. Strain isolated from chicken meat showed the human biovar, ß- lactamase production positive and resistance against 13 antibiotics while the other isolated from bovine milk showed the bovine biovar, ß- lactamase production positive and resistance against 14 antibiotics. However none of the two strains were resistant to glycopeptides. This paper is first publication regarding MRSA isolate from foods of animal origin in Algeria.
Article 83 :Année de publication 2014
Titre Physical and Chemical Characterization of Different Stages of Landfill Leachate fromAin Temouchent Engineered Landfill (Algeria)
   
Revue, référence Advances in Environmental Biology, Advances in Environmental Biology, 8(1), Pages: 18-24 . http://www.aensiweb.com/aeb.html
   
Autres auteurs Chadli Aicha
Baba Hamed Mohamed Bey
Kihal Mebrouk
   
Mots Clefs Landfill Leachate, Characterization, Pollution, Engineered, Landfill, BOD
   
Résumé
   One of the most important problems with regard to the design of the maintenance of a hiding of waste is the landfill leachate which is produced when water passes through waste. The landfill leachate includes a mass of organic and inorganic components different which can be dissolved or suspended. Independently of the nature of the components, it poses a potential problem of pollution on the local grounds and surface water. The objective of this work is to understand the genesis of the landfill leachate while following its evolution to the level of the engineered landfills of Ain Temouchent (Algeria). And for this reason we characterized from a physicochemical and bacteriological view point four samples coming from various points located at the level of the basin from accumulation of the landfill leachate (entered of the landfill leachte, surface of the basin of accumulation of landfill leachate and depth of the basin of accumulation to 1 and 5m). Physico - chemical analyzes carried out on these samples were related to: the temperature, pH, electric conductivity, proportioning of various minerals (chlorides, calcium’s, magnesium’s, sulfates, sulfites), proportioning of total hydrocarbons, organic matter (the BOD, the COD, suspended matter). We could understand the evolution of our landfill leachate studied dice its arrival with the basin of accumulation; according to the recorded results of the physicochemical and bacteriological analyzes carried out on the four samples, we can say that the landfill leachate passed through four phases: aerobic phase, transition phase, acetogenic phase and methanogenic phase. Thus we could note that this center of engineered landfills is a young discharge (report/ratio BOD55 /COD lies between 0.32 and 0.51). The determination of the physical, chemical and bacteriological characteristics of the landfill leachate of the engineered landfills of Ain Temouchent will thus make it possible to direct the die of its treatment to meet the strictest standards of rejection
Article 82 :Année de publication 2014
Titre Antifungal activity of clove (syzygium aromaticum l.) essential oil against phytopathogenic fungi of tomato (solanum lycopersicum l) in Algeria
   
Revue, référence Journal of Experimental Biology and Agricultural Sciences, Journal of Experimental Biology and Agricultural SciencesVolume – 2(5) http://www.jebas.org
   
Autres auteurs Hamini
Kadar N
Hamdane F
Boutoutaou R
Kihal M
Henni J E
   
Mots Clefs Antifungal Activity, Essential Oil, Syzygium aromaticum, Fusarium oxysporum f.sp radicis lycopercisi, Fusarium commune, Fusarium redolens
   
Résumé
   Present study was conducted to find the efficiency of Syzygium aromaticum [L.] essential oil in management of three pathogenic fungus viz Fusarium oxysporum f.sp radicis lycopercisi, F. commune and F. redolens. The yield of hydro-distilled essential oil was also computed (7.49%) in study. The results of study revealed moderate to high level antifungal activities against tested microorganisms at all the tested concentration. The highest antifungal activity was recorded at 1µLml-1 ; on this concentration clove essential oil caused complete growth inhibition of F. oxysporum f.sp radicis lycopercisi and F. redolens while the highest antifungal activity of oil against F. commune was observed at 0.5 µLml
Article 81 :Année de publication 2014
Titre Study of pectinolytic activity of Fusarium oxysporum f.sp albedinis agent responsible for bayoud in Algeria
   
Revue, référence International Journal of Agronomy and Agricultural Research (IJAAR), Vol. 5, No. 2, p. 40-45, 2014 http://www.innspub.net
   
Autres auteurs Karkachi Noureddine
Gharbi
Samia
Kihal Mebrouk
Henni Jamal Eddine
   
Mots Clefs Fusarium oxysporum f.sp albedinis, pathogenicity, pectinmethylesterase(PME), polygalacturonase (PG), citrus pectin.
   
Résumé
   The present study is devoted to the morphological characterization, pathogenicity of Fusarium oxysporum f.sp albedinis, which showed morphological variability of isolates. The study of pectin methyl esterase and polygalacturonase activities by reducing sugars dosages of 10 isolates, showed that the PME and PG activities are present in the culture filtrates of isolates and the estimated amount is much higher in the presence of citrus pectin and glucose. We noted that this activity is variable among isolates, high activity is recorded (1.59 10U/mole) in presence of glucose but it was greater than 4 times in the presence of citrus pectin, The PG activity is recorded (‘1.39 103 and 0.44 103 µegr) in the presence of 1% citrus pectin and glucose respectively
Article 80 :Année de publication 2014
Titre Study of the Antimicrobial and Probiotic Effect of Lactobacillus Plantarum Isolated from Raw Goat's Milk from the Region of Western Algeria
   
Revue, référence International Journal of Sciences: Basic and Applied Research (IJSBAR), International Journal of Sciences: Basic and Applied Research (IJSBAR) (2014) Volume 13, No 1, pp 18-27
   
Autres auteurs Mami Anas
Kerfouf Ahmed
Kihal Mebrouk
   
Mots Clefs goat’s milk; Lactobacillus; antimicrobial; activity; technologic; proprieties; bio-preservation.; probiotic
   
Résumé
   The evolution from a spontaneous fermentation to a directed one is realised with selected lactic starters which give many dairy products processing various organoleptic characters. The integration of new lactic acid bacteria strains isolated from diverse ecosystems is now used to increase the duration of bio-preservation of dairy products. Moreover, some lactic acid bacteria probiotic activity is exploited to produce functional food. The aim of this study is the research of the possible probiotic and technological potential with some preventive and therapeutic characteristics of some Lactobacillus species isolated from Algerian’s raw goat’s milk. The selected isolate was identify to species level as Lactobacillus plantarum (P6) using API 50CH Kits. Microbiological and biotechnological techniques are used to fulfill this work. Results obtained have shown that Lactobacillus plantarum (P6) can resist to acidic, basic and enzymatic stresses. So the former strain can be considered as a probiotic. Moreover, the inhibition activity of the Lactobacillus plantarum (P6) against pathogens strains (Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25921, Bacillus cereus, Pseudomonas aeruginosa, Vibrio cholerae, Listeria ivanovii ATCC 19119 and Salmonella enterica) is clearly obtained by testing these strains with the direct method. Finely, raw goat’s milk can be defined as an ecosystem that promotes the development of a microflora with probiotic characters.
Article 79 :Année de publication 2014
Titre Seroprevalence De La Brucellose Caprine et Humaine Dans La Region D’el-Bayadh
   
Revue, référence Rev. Microbiol. Ind. San et Environn., Rev. Microbiol. Ind. San et Environn. Vol 8, N°1, p : 78-88
   
Autres auteurs H. NEHARI
H . AGGAD
S. DERRER
M. KIHAL
   
Mots Clefs Brucellose, caprins, humains, El Bayadh, infection
   
Résumé
   La brucellose demeure une préoccupation majeure dans les pays arabes et méditerranéens. Plusieurs milliers de cas humains sont déclaré annuellement en Algérie. En 2008, notre étude a été menée à El Bayadh en vue d’estimer la prévalence apparente de cette zoonose chez les caprins et les humains et de rechercher l’origine de l’infection chez les humains. Les tests sérologiques ont porté sur 3865 individus caprins, répartis sur 473 fermes. L’épreuve à l’antigène tamponnée (EAT) a été effectuée. Tout caprin réagissant positivement est considéré infecté alors que pour les humains, en cas positivité, ce test est complété par la séro agglutination en tubes (SAT).Dans ce cas, la personne est considérée infectée si à la dilution 1/80 le test est toujours positif. Nos résultats ont montré une séroprévalence individuelle de 3 % et de 10,14 % pour les exploitations alors que ce sont 220 personnes qui ont été déclarées positives pour la même période (soit 113 cas /100 000 habitants).Une forte corrélation est observée entre brucellose caprine et humaine et le mode contamination humaine le plus fréquent demeure la consommation de produits laitiers dans plus de 80 % des cas. La vaccination introduite ces dernières années permettrait de diminuer l’incidence de la brucellose à condition qu’elle soit accompagnée par des mesures judicieuses.
Article 78 :Année de publication 2014
Titre 1,3,4-Oxadiazole, 1,3,4-thiadiazole and 1,2,4triazole derivatives as potential antibacterial agents
   
Revue, référence Arabian Journal of Chemistry, Arabian Journal of Chemistry 2014 www.ksu.edu.sa. www.sciencedirect.com
   
Autres auteurs Adil A. Othman
Mebrouk Kihal
Sarah Amara
   
Mots Clefs Mercapto-1,3,4-oxadiazole; 1,3,4-Thiadiazole; 1,2,4-Triazole; Antibacterial agent
   
Résumé
   Since the introduction of the first antibiotic (penicillin, 1942) into medical practice, to date, there has been an ongoing ‘‘race’’ between scientists creating new drugs and pathogenic bacteria. Antibiotic-bacteria are becoming progressively common, and to make matters worse, more and more bacteria are becoming resistant to all known antibiotics. The traditional method for this problem is to introduce new antibiotics that kill the resistant mutants. This specific ‘‘arms race’’ resulted into thousands of potentially active chemicals are synthesized in laboratories around the world every day. 1,3,4-Oxadiazole; 1,3,4-thiadiazole; 1,2,4-triazole and some of their derivatives are involved in modifications at the following axes: First, attaching a thio-group into heterocyclic rings. Second, introducing different substitutions at position 5 which often are the residuals of the synthetic starting materials such as simple aliphatic, substituted aliphatic chains, aromatic carbocyclic and heterocyclic residues.
Article 77 :Année de publication 2014
Titre Characterization and Potential Probiotic Attributes of Lactobacillus plantarum DU10 Isolated from Algerian Raw Camel Milk
   
Revue, référence Biotechnology, Year: 2014 | Volume: 13 | Issue: 6 | Page No.: 282-288 DOI: 10.3923/biotech.2014.282.288
   
Autres auteurs Zergui Amina
Saidi Noureddine
Arul Venkatesan
Venkatesh Perumal Bessaiah Hichem
Zergui Asma
Merzouk Yamina
Heddadji Miloud
Kihal Mebrouk
   
Mots Clefs Lactobacillus plantarum, Algerian Raw Camel Milk. Biotechnology, exopolysaccharide, probiotic properties
   
Résumé
   Lactic acid bacteria have long been considered safe and suitable for human consumption when administered in adequate amounts. The aim of this study is to investigate the probiotic properties of Lactobacillus plantarum DU10 isolated from Algerian raw camel milk. The analysis of haemolytic and gelatinase activity, bile tolerance, cholesterol lowering capacity, acid resistance, exopolysaccharide and biomolecule production were established. The results revealed normal growth ofLactobacillus plantarum in the presence of bile salt concentrations, gastric and intestinal fluid. This strain was able to reduce cholesterol and the majority of cells were viable at pH 2.0 even after 24 h of incubation and can produce exopolysaccharides and antimicrobial compounds. No gelatinase and haemolytic activity was detected. Natural resistance to some antibiotics was observed. According to these results, this probiotic strain could be proposed as adjunct culture and exploited for food manufacture as a biocontrol agent.
Article 76 :Année de publication 2013
Titre Characterization and determination of the factorsaffecting anti-listerial bacteriocins from Lactobacillus plantarum and Pediococcus pentosaceus isolated from dairy milk products
   
Revue, référence African Journal of Food Science, African Journal of Food Science Vol. 7(2) pp. 35-44, March, 2013 http://www.academicjournals.org/ajfs/contents/2013cont/Mar.htm
   
Autres auteurs Djadouni Fatima
Kihal Mebrouk
   
Mots Clefs Lactobacillus plantarum, Pediococcus pentosaceus, bacteriocin activity, temperature, pH, enzymes, solvents, salts, spices
   
Résumé
   Lactobacillus plantarum and Pediococcus pentosaceus strains isolated from dairy milk products, produced bacteriocins that displayed a wide spectrum of antimicrobial activity against Listeria ivanovii and food spoilage microorganisms. The two bacteriocins were thermally stable over a wide temperature range up to 100°C for 15 min and retained their activity at pH 2.0 to 6.0. Full bacteriocins activity were stable after three months of storage at 4 and -20°C, for 75 min of exposure to UV light, bacteriocins produced by P. pentosaceus was completely destroyed, but the second L. plantarum remained stable after the same time of exposure; treatment with proteolytic enzymes resulted in a remarkable stability of activity. Results obtained showed an increase in bacteriocins activity of the both strains against L. ivanovii on increasing the concentration of NaCl and KCl up to 5%, in presence of spices 5%, stability to acetone and hexane indicated that both surfaces were rather hydrophilic and bipolar. Further studies are required regarding suitable bioprocessing strategies for an efficient bacteriocin production process.
Article 75 :Année de publication 2013
Titre Caractérisation microbiologique et technologique des espèces de Leuconostoc mesenteroïdes isolées du lait cru de chèvre et de chamelle d’Algérie
   
Revue, référence Nature & Technology, Revue « Nature & Technologie ». B- Sciences Agronomiques et Biologiques, n° 08/Janvier 2013. Pages 39 à 47
   
Autres auteurs ZAROUR Kenza
HENNI Jamal Eddine
KIHAL Mebrouk
   
Mots Clefs Technological milk , Leuconostoc , growth kinetics , carbon dioxide antibiotics, yeast extract
   
Résumé
   La caractérisation microbiologique et technologique de l’espèce Leuconostoc mesenteroïdes isolée du lait cru de chèvre, de chamelle et du fromage du Roquefort ont permis d’identifier neuf souches de Ln. mesenteroïdes subsp. mesenteroïdes et neuf souches de Ln. mesenteroïdes subsp. dextranicum. Les résultats des tests technologiques ont montré que les isolats produisent le dextrane sur milieu MSE, et le dioxyde carbone, utilisent le citrate et résistent à 55°C pendant 15 minutes, ce qui favorisent leur utilisation industrielle. La souche de Ln. mesenteroïdes subsp. dextranicum a une cinétique de croissance, d’acidification et de production de dioxyde de carbone dans le lait écrémé à 30°C, un peu plus élevée que Ln. mesenteroïdes subsp. mesenteroïdes . L’addition de l’extrait de levure au lait écrémé stimule le développement de Ln. mesenteroïdes subsp. mesenteroïdes en augmentant sa croissance, son activité acidifiante et gazogène. La sensibilité des souches aux antibiotiques a été évaluée sur 33 antibiotiques et les souches ont montré une résistance à 42,4% des antibiotiques utilisés.
Article 74 :Année de publication 2013
Titre Recent Patents on Bacteriocins: Food and Biomedical Applications
   
Revue, référence Recent Patents on DNA & Gene Sequences, Recent Patents on DNA & Gene Sequences, 2013, 7, 66-73 www.benthamscience.com/dnag
   
Autres auteurs Zineb Benmechernene
Inmaculada Fernandez
N
Mebrouk Kihal
Karola B?hme
Pilar Calo
Mata
Jorge Barros
Velazquez
   
Mots Clefs Bacteriocins, , , anti-, lantiobiotics, IIa class, nisin, pediocin, Listeria, food, biopreservation
   
Résumé
   Most types of bacteria produce bacteriocins, which are proteinaceous extracellular compounds that can inhibit the growth of other undesirable microorganisms. Bacteriocins are receiving increasing attention, due to their many applications, ranging from their initial application in strategies for food preservation to more recent proposed uses in biomedical strategies aimed at fighting certain bacterial infections. Thus, while nisin has a long history of use as a safe additive in certain food products for the purpose of food preservation, certain bacteriocin-producing lactic acid bacteria, which are generally recognised as safe microorganisms, or their extracellular extracts are receiving increased attention as protective cultures or antimicrobial extracts in minimally processed food products. More recently, a number of these bacteriocinproducing cultures have been proposed for use in other applications, such as in probiotics, for the inhibition of biofilms in the food industry, or even as coadjuvants of combined therapeutical strategies along with other antimicrobial agents in biomedical applications. This review aims to provide a brief overview of the most relevant recent patents in this field
Article 73 :Année de publication 2013
Titre Identification and genetic diversity of Bacillus cereus strains isolated from a pasteurized milk processing line in Algeria
   
Revue, référence Dairy Sci. & Technol., Dairy Sci. & Technol. (2013) 93:73–82 www.dairy-journal.org/
   
Autres auteurs F. Malek B. Moussa Boudjemaa A. Aouar
Métri & M. Kihal
   
Mots Clefs Bacillus cereus, M13-PCR typing, Milk powder, Pasteurized milk, Phylogenetic, Dairy
   
Résumé
   Bacillus cereus is a food pathogen of major concern to the dairy industry. Strains of B. cereus were isolated in 2006 and 2010 from a pasteurized milk processing line in a dairy plant in Algeria. Pasteurized milk is obtained from reconstituted and processed milk powder. This study was designed to discriminate between the isolated B. cereus strains and to predict their food poisoning potential. Strains were fingerprinted by M13-PCR, and identified at the phylogenetic group level by amplified panC gene sequence analysis. They were clustered into three distinct M13-PCR groups: one major group which included 17 strains and two minor groups which contained two and one strains, respectively. Strains originating from both the process equipment and milk powder were from the major group indicating that milk powder was the main source of initial contamination. Strains from the major and second group were affiliated to the mesophilic phylogenetic group III while the unique strain of the third group was classified into the mesophilic phylogenetic group IV. The data presented in this study showed a very low genetic diversity among B. cereus strains identified in milk powder and secondly by milk processing systems, as well as persistence in the dairy environment of specific B. cereus genotypes, across 4 years. M13-PCR typing and phylogenetic affiliation were useful for characterizing B. cereus dairy isolates, permitting their differentiation within the B. cereus group, and showing homogeneous contamination throughout the pasteurized milk processing line. The recurrent genotypes which belong to the potentially toxigenic group III B. cereus could threaten pasteurized milk safety.
Article 72 :Année de publication 2013
Titre Phenotypic and Genotypic Characterization of Staphylococcus aureus Agents of Dairy Cows’Mastitis in Algeria
   
Revue, référence Journal of Applied Sciences Research, Journal of Applied Sciences Research, 9(1): 86-93, Http://www.aensiweb.com/jasr.html‎
   
Autres auteurs Nadjia Benhamed Mebrouk Kihal
   
Mots Clefs Mastitis, Staphylococcus aureus, identification, PCR, mecA, pvl.
   
Résumé
   Mastitis is an inflammation of the mammary gland with local and or symptoms that occasionally result in a systemic infection. This disease has aprofound impact on animal welfare and milk quality. The aim of this work was to investigate the microbial etiology and the occurrence of antimicrobial resistance. Moreover Difference between Staphylococci antimicrobial resistance isolated subcliniques cases and clinical cases was investigated. In total, 213 quarter milk samples were collected from 56 dairy cows at 6 farms from February 2011 to February 2012. The quarter milk samples were bacteriological analyzed and scored using California Mastitis Test. The most frequently isolated bacteria were the Gram negative bacteria, staphylococci and streptococci, the presence of resistance was evaluated in all specific udder pathogens. Staphylococci antimicrobial resistance was performed by detection of mecA gene and the Pantin Valentine leukocidin coding gene (pvl) by PCR. The most common isolated of 250 bacteriological diagnoses were Enterobacteria (45.76%) Staphylococcus aureus (38.9%), coagulase-negative staphylococci (2.7%) and Streptococcus (12.43%) in milk samples from clinical cases (47.87%) and subclinical cases (52.12%) infected cows. All retained Staphylococcus aureus species contained gyr gene. Overall the S. aureus isolates were sensible to antimicrobials compounds. Staphylococcus, Escherichia coli and Enterobacter cloacae were the most frequently isolated pathogens .The testing of Staphylococcus antimicrobial resistance isolated subclinical and clinical cases showed the absence of resistance to antimicrobials agent.
Article 71 :Année de publication 2013
Titre Physico-chemical and Microbiological Analysis of Algerian Raw Camel’s Milk and Identification of Predominating Thermophilic Lactic Acid Bacteria
   
Revue, référence Journal of Food Science and Engineering , Journal of Food Science and Engineering 3 (2013) 55-63 www.davidpublishing.org/journals
   
Autres auteurs Merzouk Yamina Chahrour Wassila
Zarour Kenza Zergui Amina
Saidi Noureddine Henni Jamal Eddine Kihal Mebrouk
   
Mots Clefs Raw camel’s milk, physicochemical analysis microflora, electrophoretic analysis, proteins of milk
   
Résumé
   The dromedary camel (Camelus dromedarius) is a significant socioeconomic importance in several arid and semi-arid regions of North Africa and Middle East, and its milk constitutes an important component of human diets in these regions. The camel milk plays a vital role in the food of the Algerian nomads in the Sahara. During February and September, 20 samples of the raw camel’s milk were taken starting from different livestock of camels from three different Sahariennes regions (Bechar, El-Bayadh and Naama). These 20 collected samples were analyzed by physico-chemical and microbiological methods. The results of physicochemical analyze obtained from two hot and cold seasons are respectively the following: T °C (35.83 and 33.95), pH (6.36 and 6.49), density (1.031 and 1.032), dornic acidity (18.6 and 18.3 °D), dry matter (93.4 and 144.8 g/L), fat contents (30 and 52.1 g/L), total protein (26.3 and 33.1 g/L) and ashes (7.46 and 8.66 g/L). The protein profile obtained by electrophoretic analysis (SDS-PAGE) showed that camel milk contains several types of proteins and some have a molecular weight identical to major proteins of the cow’s milk. The final results showed that camel milk has generally a comparable composition to that of bovine milk. The microbiological analysis, of these samples, detected a significant number of the total microflora, Staphylococcus aureus and total coliforms. The absence of Clostridium and fecal coliforms was observed. Several species of lactic acid bacteria were detected such as Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis biovar. diacetylactis, Weissella cibaria and Enteroccocus feacalis
Article 70 :Année de publication 2013
Titre Screening and identification of lactic acid bacteria isolated from sorghum silage processes in west Algeria
   
Revue, référence African Journal of Biotechnology, African Journal of Biotechnology Vol. 12(14), pp. 1703-1709, 3 http:// www.academicjournals.org/.../abs2013/.../Chahrour
   
Autres auteurs Chahrour W. Merzouk Y. Henni J. E Haddaji M. Kihal M.
   
Mots Clefs Lactic acid bacteria, identification, silage sorghum, evolution, amylolytic, technology, species
   
Résumé
   The lactic acid bacteria (LAB) isolated from sorghum (Sorghum bicolor. L.) silage were identified during different periods of evolution of sorghum silage in west Algeria. Morphological, physiological, biochemical and technological techniques were used to characterize lactic acid bacteria isolates. A total number of 27 representatives of lactic acid bacterial strains were retained and among them four dominant genus were identified as Lactobacillus (44%), Lactococcus (14.81%), Weissella (29.62%) and Leuconostoc (11.11%). The representative species identified were Lactobacillus brevis (25%), Lactobacillus pentosus (3.7%), Lactobacillus manihotivorans (11.11%), and Lactobacillus fermentum (3.7%). Lactococcus lactis subsp. lactis biovar. diacetylactis (14.81%), Weissella cibaria (7.2%), Weissella minor (11.11%), Weissella soli (3.7%), Weissella viridescense (7.2%) and Leuconostoc mesenteroides subsp. mesenteroides (11.11%). Only two strains of lactic acid bacteria were amylolytic. These results will enable future research on the relationship between LAB species and silage fermentation quality.
Article 69 :Année de publication 2013
Titre Biodiversity of molecular profile of Staphylococcus aureus isolated from bovine mastitis cases in West Algeria
   
Revue, référence Journal of Bacteriology Research, Journal of Bacteriology Research Vol. 5(3) http:// www.academicjournals.org/.../abstracts2013/.../Benha...‎
   
Autres auteurs Nadjia BENHAMED Mebrouk Kihal
   
Mots Clefs Mastitis, S. aureus, Gyr A, PCR mecA, pvl.
   
Résumé
   Bovine mastitis is an inflammation of the mammary gland with local and or symptoms that occasionally result in a systemic infection. This disease has a profound impact on animal welfare and milk quality, and the most costly disease affecting dairy cows. The bacteria Staphylococcus aureus is one of the most frequently isolated pathogens from both subclinical and clinical infections. This study was conducted to investigate the phenotypic and genotypic characterization of S. aureus involved in dairy cow mastitis in West Algeria. A total of 141 isolates of S. aureus isolated from quarter milk samples were collected from dairy cows. All retained S. aureus species contained gyr gene and were identified by molecular typing. The presence of resistance was evaluated in S. aureus. Staphylococci antimicrobial resistance was performed by detection of mecA gene. Several virulence factors including toxin of the Pantin Valentine leukocidin coding gene (pvl) were also investigated by polymerase chain reaction (PCR). Only one strain of S. aureus was mecA - and pvl+ gene.
Article 68 :Année de publication 2013
Titre Antifungal compounds production in differentes temperatures pH and modified MRS agar by Lactobacillus strains
   
Revue, référence Journal of Biological Sciences, Journal of Biological Sciences, 13.2 :94-99 http://scialert.net/jindex.php?issn=1727-3048
   
Autres auteurs Laref Nora Guessas Bettache Kihal Mebrouk
   
Mots Clefs Lactic acid bacteria, Antifungal, Aspergillus, inhibition
   
Résumé
   The effect of abiotic and biotic factors on the antifungal activity was evaluated, pH and on modified MRS (de Man Rogosa Sharpe). Latic Atic Bacteria (Lab) were able to produce antifungal compounds under different temperatures, in 30°C showed a good inhibition because no conidia was observed; in this temperature all isolates when grown at pH and 4 show a stronger antifungal activity. the best inhibition in the presence and absence of sodium acetate was observed on MRS without meat extract and glucose, although the amount of activity produced does vary depending on the carbon source.
Article 67 :Année de publication 2013
Titre Identification and Physiological Properties of Bifidobactérium Strains Isolated from Different Origin
   
Revue, référence Journal of Food Science and Engineering, Journal of Food Science and Engineering 3: 196-206 http://www.davidpublishing.org/journals_info.asp?jId=646‎
   
Autres auteurs Fatima Mahmoudi Hadadji Miloud Guessas Bettache Kihal Mebrouk
   
Mots Clefs Probiotic, Bifidobacterium, acidity, survival, growth, isolate
   
Résumé
   Bifidobacteria play an important role in maintaining human health, and suppress harmful bacteria by controlling the pH of the large intestine. About 14 strains of the Bifidobacteria have been isolated from the faeces of infants nursed exclusively from the maternal milk (their ages less than 5 months) from commercial French yoghurt (active bifidus] and from milk (celia bifidus) on MRS medium (containing 0.05% cysteine-HCl, with nalidixic acid 2 mg m-1). A total of six strain are strict anaerobic and gram-positive Bifidobacteria, identified as, B. longum (60% of the samples) was the most frequently found species, followed by B. bifidum (21.0%), B. breve (19%). These strains were evaluated for potential use as probiotics based on their adhesion to intestinal epithelial cells, resistance towards acidic (resistance to low pH) and bile conditions. pH values ranging from 6.5 to 7, however, these strains are sensitive to the pH 8, Bifidobacterium are mésophiles with optimal growth at 36 °C to 41 °C. All isolates studied have a fast growth in glucose.
Article 66 :Année de publication 2013
Titre Evaluation of Microbiological Quality of the Date Fruit Product “Btana” Produced in Adrar South Algeria
   
Revue, référence Journal of Microbiology Research, Journal of Microbiology Research 3(5) : 163-170 http://www.sapub.org/journal/currentissue.aspx?journalid=1094
   
Autres auteurs Abekhti A Zarour K Boulal A Benmechernene Z Kihal M.
   
Mots Clefs Date Fruit, Storage, Microflora, Quality, Indicator, Date Palm, Btana
   
Résumé
   Dates, the fruits of date palm (Phoenix dactylifera L.) a re a vit a l element of the dai ly diet in the Arabian world. Dates over production needs to be transformed into local traditional products like “Btana”. Our study aimed to describe the dynamic changes of mic robial and chemical parameters (pH, water content) of dates during transformation and storage period. Sa mples were collected fro m different processing and storage periods fro m 4 villages in Adrar province located at the s outh of Algeria . Microbia l analys is showed that La mai ze ra w dates recorded a high number of APc count (3. 5 x 10 ufc/g). In addition Abani samples included a level of faecal col ifo r ms (40 ufc/g) wh ich were absent in other samples. API 20 E assay confirm the presence of Klebsiella (K. terrigena, K. pneumonia and K. oxytoca) and Yersinia enterocolica. At the end of the storage period, an important decrease in microbia l population was recorded. The h ighest reduction of APc was observed in Mraguen s ample (fro m 3,5x106 to 5x102 ufc/g ) during four wee ks of storage. Fungal population s ignificant ly d i minis hed at the fourth week of storage. Bot h pathogens and bacterial indicators detected on raw dates (S. aureus, faecal coliforms) were complet e ly absent in the stored Btana.
Article 65 :Année de publication 2013
Titre Carbon sources and pH effect on pectinolytic activity production by Ascochyta rabiei isolated from chickpea (Cicer arietinum L.) in West Algeria
   
Revue, référence African Journal of Microbiology Research, Afr. J. Microbiol. Res. Vol. 7(27), pp. 3483-3488, 5 http://www.academicjournals.org/
   
Autres auteurs Gharbi Samia Karkachi Noureddine Kihal Mebrouk Henni Jamal Eddine
   
Mots Clefs Ascochyta rabiei, pectinolytic activities, pectin, méthylesterase, polygalacturonase
   
Résumé
   In Algeria, Ascochyta blight is a major limiting factor in chickpea production. We did not record any difference between the isolates while basing ourselves on the morphological and cultural characters, but the pectinolytic activity could differentiate the ten isolates of Ascochyta rabiei. Activity of pectin methyl esterase was baseless in the presence of glucose, but variable in the presence of the citrus pectin and polygalacturonic acid. We recorded the weak production ranging between 0.19 and 0.55 µeg/ml/min; however the strong activity was between 2.97 and 5.88 µeg/ml/min in the presence of citrus pectin and polygalacturonic acid, respectively. On the other hand, the polygalacturonase activity was present in culture filtrates using three sources of carbon. Weak production was recorded in the presence of glucose (0.28 to 2.63 µmole/min), however the strong activity was recorded in the presence of 16.29 µmole/min of polygalacturonic acid. The maximal production of polygalacturonase was 11.7 µmole/min in the presence of the polygalacturonic acid and 0.9 µmole/min in the presence of glucose after six days of incubation at pH 5. The polygalacturonase activity was between 0.3 and 0.62 µmole/min after 4 days of incubation at pH 8. However, optimum production was obtained at pH 5.5. It should be noted that there is an important difference in the production of polygalacturonase with simple sugars glucose and pectin, indicating that the production of the enzyme is also stimulated by the presence of inducers. The exo-polygalacturonase is present in all carbon sources, suggesting that this enzyme could probably be of a constitutive nature in Ascochyta rabiei.
Article 64 :Année de publication 2013
Titre Evaluation of microbiological and sanitary quality of ewe’s raw milk in Western of Algeria and detection of antibiotic residue by Delvotest
   
Revue, référence Advances in Environmental Biology, Advances in Environmental Biology, 7(6): 1027-1033 http://www.aensiweb.com/aeb.html
   
Autres auteurs Beldjilali Asmaa Fatima Benlahcen Kheira Guessas Bettache Aggad Habib Kihal Mebrouk
   
Mots Clefs ewe milk, microbiological, quality, hygiene, bacterial count
   
Résumé
   In this study, a total of 105 ewe’s raw milk of Ouled-Djellal breed were collected from three farms (Oran, Relizane and Mascara) in western of Algeria. They were analyzed for microbiological and physico-chemical characteristics. The pH, titriable acidity, temperatures and detection of antibiotics using the Delvotest were determined. For the study, a questionnaire was filled to collect information about herd sheep characteristics. Microbiological examination involved the estimation of total mesophilic aerobic flora (FAMT), total and fecal coliforms, Enterobacteriacea, fecal Streptococcus, Staphylococcus spp., Sulphite Reducing Clostridia (SRC), Salmonella, Yeast and Lactic Acid Bacteria as well as the prevalence of mastitis infection using California mastitis test. The average values of pH, titrable acidity and temperatures were (6.84, 17.87°D and 36.9°C), (6.66, 18.13 °D and 37.2°C) and (6.68, 18.7°D and 37.6°C) in Oran, Relizane and Mascara farms respectively. The antibiotics residues were found in 4.76% of milk samples. The results revealed that Oran’s samples recorded the highest count of FAMT (90.2 x103 cfu/ml), Entrobacteriacea (10.8 x102 cfu/ml) and Staphylococcus (17.9 x102 cfu/ml), whereas the mean count of fecal Streptococcus, total and fecal coliforms were highest in Mascara’s samples with 8.98 x102, 13 x102 and 10.9 x102 cfu/ml respectively. Lactic acid bacteria, yeast and fungi were detected in all milk samples with high mean count. A total absence of Sulphite Reducing Clostridia (SRC) and Salmonellae was noted. Mastitis infection was detected in 37.14% of the sheep herd. In this study, the count of indicator bacteria exceeds the charges allowed by the national and international standards. Contamination of milk can be eliminated by the respect of strict hygienic measures in local farms.
Article 63 :Année de publication 2013
Titre Diversity And Technological Properties Of Predominant Lactic Acid Bacteria Isolated From Algerian Raw Goat’s Milk
   
Revue, référence Advances in Environmental Biology, Advances in Environmental Biology, 7(6): 999-1007 http://www.aensiweb.com/aeb/2013/999-1007.pdf
   
Autres auteurs Moulay M. Benlahcen K. Aggad H. Kihal M.
   
Mots Clefs Goat, raw milk, lactic acid, bacteria, Identification, Isolation, Acidity
   
Résumé
   Lactic acid bacteria (LAB) are a group of gram-positive, lactic acid producing firmicutes. They have been extensively used in food fermentations, including the production of various dairy products. The proteolytic system of LAB converts proteins to peptides and then to amino acids, which is essential for bacterial growth and also contributes significantly to flavor compounds as end-products. The objective of this paper is the microbiological and technological characterization of lactic acid bacteria isolated from Algerian raw goat’s milk. Microbiological, physiological and biochemical tests were made for the work performed. Dominant lactic acid bacteria were isolated on M17 and MRS media from Algerian goat's milks, either raw and/or fermented under laboratory conditions. Isolates were characterized for three phenotypes essential for Raib manufacture: (a) high acidifying activity over the normal associated temperature range, (b) presence of protease, and (c) ability to metabolize citrate. Thirty nine isolates were characterized and 13 strains which have been found to belonging to lactic acid bacteria including the species of (Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris), Leuconostoc (5 isolates) and Lactobacillus (6 isolates). These strains lactic have technological characteristics acidification and interesting flavor. These characters of industrial interest can be exploited to provide a specific starter for the local dairy industry.
Article 62 :Année de publication 2013
Titre The reproducibility of random amplified polymorphic DNA (RAPD) profiles of Streptococcus thermophilus strains with XD9, M13 and OPI-02 MOD primers
   
Revue, référence African Journal of Biotechnology, Vol.12(44), pp. 6245-6252. http://www.academicjournals.org/
   
Autres auteurs Fatima Ghazi Zineb Benmechernene Mebrouk Kihal G. Candan Gurakan
   
Mots Clefs Streptococcus thermophilus, random amplified polymorphic DNA (RAPD), DNA template, Taq DNA polymerase, OPI-02MOD, XD9, M13, optimization, reproducibility
   
Résumé
   We optimized the conditions for generating random amplified polymorphic DNA (RAPD) profiles of Streptococcus thermophilus strains by using the polymerase chain reaction (PCR). Several factors can cause the amplification of false and non reproducible bands in the RAPD profiles. We tested three primers OPI-02 MOD, M13 and XD9 throughout this study. Additionally, we tested different concentrations of primer, DNA template and Taq DNA polymerase. We adjusted the ratio of the primer to DNA template. All the three primers yielded reproducible profiles on several days, under optimized concentrations of components and cycling parameters used. The bands of such profiles probably corresponded to perfect annealing sites amplified with good efficacy or present in multiple copies in the genome. Five months later, repeated experiment generated identical bands. However, extra faint bands were detected with M13 and XD9 primers, possibly, corresponding to nonspecific binding resulting from slight variation in temperature or calibration of the thermocycler. Therefore, OPI-02 MOD was determined as the most reliable primer for reproducible profiles of S. thermophilus strains
Article 61 :Année de publication 2013
Titre Phylogenetic and proteomic characterization of bioactive Leuconostoc mesenteroides strains isolated from raw camel milk
   
Revue, référence Canadian Journal of Microbiology, Canadian Journal of Microbiology http://mc06.manuscriptcentral.com/cjm-pubs
   
Autres auteurs Zineb Benmechernene Inmaculada Fern?ndez
No Marcos Quintela Fatma Chentouf Karola B?hme Mebrouk Kihal Pilar Calo
Mata Jorge Barros
Vel?zquez
   
Mots Clefs Raw camel milk, biopreservation, Leuconostoc mesenteroides, lactic acid bacteria, minimally-processed, foods
   
Résumé
   Abstract: In this study, bioactive lactic acid bacterial (LAB) strains were isolated from raw camel milk in Southern Algeria, to our knowledge for the first time. Thirteen isolates exhibited anti-listeria activity, also exhibiting inhibitory effect against other LAB such as Lactobacillus spp. and Lactococcus spp. and against pathogenic bacteria such as Escherichia coli and Staphylococcus aureus. All isolates were citrate positive, able to produce CO from . glucose, produced dextran from sucrose and were unable to hydrolyse arginine. Fermentation profiling showed that all isolates were able to ferment glucose and lactose, but exhibited some differences in their ability to ferment other sugars. Phylogenetic analysis indicated that all isolates were grouped in a common branch with reference Leuconostoc mesenteroides strains, thus confirming their identity as L. mesenteroides. The isolates were also characterized by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI- TOF MS), which identified two mass peaks at 4442.78 m/z and 5117.81 m/z that were characteristic of these L. mesenteroides strains, not being present in other Leuconostoc spp. Phyloproteomic analysis allowed for the grouping of L. mesenteroides strains into different clusters. This study represents the first report on the phenotypic and molecular characterization of bioactive L. mesenteroides with potential technological interest, isolated 36 from raw camel milk.
Article 60 :Année de publication 2013
Titre Study of the microbiological and physicochemical quality of raw milk from cows exposed to environmental pollutants in the region of west algeria
   
Revue, référence International Journal of Environmental Engineering Science and Technology Research , International Journal of Environmental Engineering Science and Technology Research 1, 9: 229-240, ISSN: 2326-3113. 2013 (www.ijestr.org)
   
Autres auteurs Benlahcen K Mouloudi F Kihal M.
   
Mots Clefs Raw milk, Chemical pollution, Neurobiology of behaviour, Wistar rats, Hormone, Stress
   
Résumé
   The environment is threatened constantly by noise and industrial pollutants including toxic fumes and heavy metals (arsenic, cadmium, lead and zinc etc...). In addition, high household hazardous wastes are dumped haphazardly in the open air. For lack of grass hay and water, it is in these places that some cows in Sidi Bachir on the outskirts of the Wilaya of Oran find their pasture. This research has two components: 1) Microbiological and physicochemical study of raw milk of cows feeding mainly on waste dumped in nature. 2) A Behavioral study that for ethical reasons the experiment is performed in a laboratory at the University of Oran, on thirty (30) Wistar rats. The Group A is subject to poisoning lead acetate (a heavy metal that can probably be found in the place of pasture) at a dose of 500 mg/ l, and the Group T (control rats) , which receives clean tap water. The results show neurological and behavioral disorders, which is in favour of the involvement of serotonergic transmission system. 3) In order to deepen the involvement of the different routes of transmission, we undertook a Biochemical study to reveal stress hormones: prolactin in female rats and corticosterone in male rats. The results show in the first place, evidence of microbial contamination of raw milk with high levels of impurities, whose presence is probably due to non-existent hygiene practices in the areas of housing of animals, grazing and milking. Second, chemical pollution is causing a decrease in nerve transmissions (catecholamine, glutamate and serotonin). Finally, hormonal dosage of corticosterone and prolactin showed that lead causes a decrease in fertility in addition to a state of anxiety and stress in animals.
Article 59 :Année de publication 2013
Titre Evaluation of the Factors Affecting the Variation of the Physicochemical Composition of Algerian Camel’s Raw Milk During Different Seasons
   
Revue, référence Advances in Environmental Biology, Advances in Environmental Biology 7(14) December 2013, Pages: 4879-4884 : http://www.aensiweb.com/aeb.html
   
Autres auteurs Rahli Fouzia
Saidi Noureddine
Kihal Mebrouk
   
Mots Clefs Dromedary, Physico-Chemical, Camelbreeding, Forage, Dry lands
   
Résumé
   The camel is of considerable socio-economic value in many arid and semi-arid areas of the world and its milk comprises a significant part of human dietary habits in these regions. Camel milk is just like the bovine milk in terms of its essential nutriments and since ancient times it is being used for curing a number of diseases. In order to determine the effect of some factors, as feeding and season on the physico-chemical composition of the milk, a total of twenty seven samples of raw camel’s milk were collected from Bechar in the south of Algeria during different seasons. Milk was analyzed for its chemical composition fat, dry, lactose, and ash and for its physical characteristics: pH, acidity and density. A survey was conducted before to determine factors influencing the variety of the milk composition. These analyses showed that collected milk presents a variety in its composition during the seasons namely: temperature, pH, acidity and density ranged from (38 to 38.7°C, 6.60 to 6.8, 16.28 to 18.3°D, 1.0227 to 1.0310) respectively. The values for fat matters, dry matters, lactose and ash were between (31.8 and 40.2, 98.4 and 119.05, 30.82 and 38.87, 7.2 and 8.6 g/L) respectively. Regarding seasonal variation, maximum level of fat, dry and ash was observed in sample 2 (winter) and sample 3 (spring) while the minimum in sample 4 at summer time thus as in sample 1 (autumn). For lactose, the maximum mean value was obtained in sample 4 (summer) and the minimum value in sample 2. The ash content was high in sample 2 (winter) then stable all over the year. The nutritional value of forage species that representthe only available food resource for the dromedary also varies during the season. The great variation in camel milk composition was attributed first to the feeding and season which are directly related.
Article 58 :Année de publication 2013
Titre Characterization of Indigenous and Adapted Hydrocarbon Degrading Bacteria Isolated from Landfill Leachate from Ain Temouchent Engineered Landfill, Algeria
   
Revue, référence Journal of Environmental Science and Engineering, Journal of Environmental Science and Engineering A 2 537-548
   
Autres auteurs Chadli Aicha
Baba Hamed Mohamed Bey
Kihal Mebrouk
   
Mots Clefs Landfill leachate, crude oil, hydrocarbons, biodegradation, bacteria
   
Résumé
   One of the most important issues regarding the design of engineered landfill is landfill leachate that is produced when water passes through the waste. Landfill Leachate comprises a mass of different organic and inorganic components which can be dissolved or suspended. Regardless of the nature of the components, it poses a potential pollution on local land and surface waters. The present work was undertaken to assess, isolate, identify and characterize the hydrocarbon degrading bacteria associated with environmental samples collected from discharge of Ain Temouchent, Algeria. The samples (landfill leachate) were chemically and microbiologically analyzed using standard techniques. These organisms were further studied to determine the level of biodegrading activities on hydrocarbons as the single carbon source using enrichment medium under different physical and chemical parameters (pH, temperature, NaCl and tolerance of crude oil…). The identification of seven strains isolated named as Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas sp., Staphylococcus aureus, Staphylococcus xylosus, Staphylococcus hominis and Staphylococcus warnerii. These strains growth on a neutral medium and temperature between 30 °C and 37 °C, 5 at 6% of NaCl concentration, and can use a concentration of 5 at 10% of crude oil as sole source of carbon.
Article 57 :Année de publication 2012
Titre Screening of autochthonous Lactobacillus species from Algerian raw goats’ milk for the production of bacteriocin-like compounds against Staphylococcus aureus
   
Revue, référence African Journal of Biotechnology, Vol. 11(20), pp. 4595-4607, 8 March,
   
Autres auteurs Mami Anas
Henni Jamal Eddine
Kihal Mebrouk
   
Mots Clefs Raw goat’s milk mixed culture, lactic acid bacteria, Lactobacillus plantarum, interaction Staphylococcus, bacteriocin
   
Résumé
   Lactic acid bacteria play a key role in maintaining the balance of normal gastro-intestinal microflora. Food contamination by Staphylococcus aureus is a major problem for consumer’s health in Algeria, especially during the summer period. The use of bacterial interactions is a new way to limit the pathogenic germs growth. Detection of antimicrobial substances produced by lactic acid bacteria against the undesirable germs is the objective of this work. Microbiological and biochemical methods were used to identify lactic acid bacteria having an antimicrobial activity. The 02 isolates of lactic acid bacteria obtained from raw goats’ milk in western Algeria areas were identified as: Lactobacillus rhamnosus and Lactobacillus plantarum. The interactions study revealed that two lactobacilli species: L. plantarum (58) and L. plantarum (68) are able to inhibit S. aureus’ growth. In mixed culture after 24 h, L. plantarum reduces the growth of S. aureus by 1.6 log and this latter bacteria was not found after 72 h. The various tests used revealed the proteinic nature of the substance, which was responsible of the growth inhibition of S. aureus. The ecological adaptation and growth characteristics of cultures of L. plantarum in food products will determine their effectiveness as biocontrol agent in dairy foods.
Article 56 :Année de publication 2012
Titre Bioprospecting of Leuconostoc mesenteroides strains isolated from Algerian raw camel and goat milk for technological properties useful as adjunct starters
   
Revue, référence African Journal of Microbiology Research, African Journal of Microbiology Research Vol. 6(13), pp. 3192-3201, 9 April,
   
Autres auteurs Zarour K.
Benmechernene Z.
Hadadji M
Moussa
Boudjemaa B
Henni D. J.
Kihal M
   
Mots Clefs Technological, yeast extract, Leuconostoc, milk, antibiotics, growth kinetic, carbon dioxide
   
Résumé
   Leuconostoc species are lactic acid bacteria widely used in milk fermentation. Based on morphological, physiological and biochemical analysis, 18 strains of Leuconostoc mesenteroides were isolated and identified from 10 samples of goat's milk and camel's milk. Strains were identified as follows 09 strains of L. mesenteroides subsp. mesenteroides and 09 strains of L. mesenteroides subsp. dextranicum. The results of technological tests of the strains showed that strains produced dextran, carbon dioxide, and resist to 55°C for 15 min, which promote their industrial use. The growth kinetic, acidification evolution and carbon dioxide production of L. mesenteroides subsp. dextranicum strain in skim milk at 30°C were slightly higher than L. mesenteroides subsp. mesenteroides. Addition of yeast extract to skim milk stimulates the development of L. mesenteroides subsp. mesenteroides by increasing its growth, acidification activity and evolved CO2. Susceptibility to antibiotics was also evaluated on 33 antibiotics and strains of L. mesenteroides showed resistance to 42.4% of antibiotics used.
Article 55 :Année de publication 2012
Titre Isolation and Identification of Lactic Acid Bacteria from Dhan, a Traditional Butter and Their Major Technological Traits
   
Revue, référence World Applied Sciences Journal , World Applied Sciences Journal 17 (4): 480-488,
   
Autres auteurs Guessas Bettache
Adjoudj Fatma
Hadadji Miloud
Kihal Mebrouk
   
Mots Clefs Lactobacillus, Dhan, Traditional fermented, milkbutter, Households
   
Résumé
   A total of 5 samples of traditional fermented milkbutter (Dhan) were collected from individual households. Lactic acid bacteria dominated the microflora of these samples, especially the genera Leuconostoc, Lactococcus and Lactobacillus. Other groups identified included pyogenic streptococci and enterococci. The dominant lactococci species was Lactococcus lactis subsp. lactis. Eighty-three percent of the Leuconostoc isolates were identified as Leuconostoc mesenteroides subsp. dextranicum. Other species identified included Leuconostoc citreum, Leuconostoc lactis, Lactobacillus delbrueckii subsp. lactis and Lactobacillus plantarum
Article 54 :Année de publication 2012
Titre Isolation, identification, and technological characterization of wild leuconostocs and lactococci for traditional Raib type milk fermentation
   
Revue, référence Dairy Sciences. & Technology, Dairy Sciences. & Technology. Vol 92, N° 3 , 249-264
   
Autres auteurs Nahida Bendimerad
Mebrouk Kihal
Françoise Berthier
   
Mots Clefs Leuconostocs .. , Raib type milk, Lactococci, Enterococci ., fermentation, Rep-PCR
   
Résumé
   Raib (Rayeb) is a spontaneously fermented milk primarily acidified and flavored by strains of lactococci and leuconostocs. Raib and its by-products are traditionally consumed in many Mediterranean and sub-Saharan countries. Dedicated ready-to-use starter strains are currently missing. The aim of this study was to isolate and characterize wild strains of lactococci and leuconostocs that could be used to produce these dairy products while preserving their traditional characteristics. Dominant lactic acid cocci were isolated on M17 and MRS-vancomycin plates from Algerian cow's, ewe's, goat's, mare's, and camel's milks, either raw and/or fermented under laboratory conditions. The DNA of the isolates was fingerprinted by Rep-PCR. Strain identification was performed by Rep-PCR combined with specific PCR for genus Enterococcus and subspecies lactis/hordniae and cremoris of Lactococcus lactis. Isolates were characterized for three phenotypes essential for Raib manufacture: (a) high acidifying activity over the normal associated temperature range, (b) presence of protease, and (c) ability to metabolize citrate. Eighty-four isolates were characterized including four different strains of lactococci and four different strains of leuconostocs. Vancomycin-resistant enterococci were often coisolated. One strain of Leuconostoc mesenteroides exhibited an atypical genotype compared to the subspecies-type strains and to other wild Leuconostoc strains. Two protease-positive strains of Lactococcus lactis subsp. lactis biovar diacetylactis isolated from camel's fermented milks and the strain Leuconostoc mesenteroides subsp. mesenteroides isolated from mare's fermented milks had high acidifying potential in milk. These three strains were considered as suitable candidates as acidifying starters to preserve the typical sensory characteristics of traditional Raib while improving its safety and shelf life.
Article 53 :Année de publication 2012
Titre Microflora of biofilm on Algerian dairy processing lines: An approach to improve microbial quality of pasteurized milk
   
Revue, référence African Journal of Microbiology Research , African Journal of Microbiology Research Vol. 6(17), pp. 3836-3844, 9 May, 2012
   
Autres auteurs Malek F.
B. Moussa
Boudjemâa
F. Khaouani
Yousfi
A. Kalai
M. Kihal
   
Mots Clefs Milk contamination, pasteurization, biofilm, cleaning-in-place (CIP).
   
Résumé
   Bacterial contamination of pasteurized milk may originate from different sources: raw milk, process equipment surfaces and packaging materials. It is hypothesized that post-pasteurization contamination along the milk processing lines is responsible of reducing shelf life of Algerian pasteurized milk. This assumption was investigated through assessment of both the microflora of biofilms in milk pipeline systems at five dairy plants of Northwestern Algeria and the effectiveness of a quaternary ammonium based compound used for the disinfection of the plant equipment. Samples were collected before and after cleaning-in-place (CIP) systems from different segments of pasteurization lines with sterile cotton swabs. Quantitative assessment showed little reduction of the total bacteria count after CIP. On the average bacterial numbers were 5.6 × 103, 1.2 × 104, 5.1 × 104, 2.5 × 105 and 9.7 × 107 cfu/cm2, respectively, in the different units. Phenotypic identification of isolates revealed predominance of Gram-positive bacilli belonging to Bacillus and notably the Bacillus cereus group, at maximal levels of 72 and 21% respectively. The other Gram-positive microflora included Staphylococcus (30%) and Micrococcus (10%). In contrast, the incidence of the Gram-negative bacteria was relatively low. Two genera, identified as Pseudomonas (9%) and Enterobacter (6%), were found only in two dairies. Three dairies were Gram-negative bacteria-free. That should be the result of common contamination sources or highly environmental selective pressure. Further studies have to address these hypotheses. Treatment of experimental Bacillus cereus sensu lato strains biofilms with a 50, 100 and 150 ppm of quaternary ammonium disinfectant, showed a significant resistance of biofilms to this product even after long exposure time (15 min). This study emphasized the importance of aerobic spore-forming bacteria in dairy-processing equipment as they are able to built recalcitrant biofilms on the inside equipment surfaces with subsequent resistance to conventional CIP system and potential transfer to pasteurized milk. Therefore, in order to reduce the contamination levels of spore-forming bacteria and improve the quality and shelf life of the product, these dairies have, besides improvement in the hygienic status of the plant equipments, also to monitor either the pasteurization process or the contamination from raw material (that is, milk powder)
Article 52 :Année de publication 2012
Titre Antimicrobial Activity of Lactic Acid Bacteria and the Spectrum of their Biopeptides Against Spoiling Germs in Foods
   
Revue, référence BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY Vol.55, n. 3: pp.435-443, May-June 2012 http://www.scielo.br/scielo.php?pid=1516-8913&script=sci_serial
   
Autres auteurs Fatima Djadouni
Mebrouk Kihal
   
Mots Clefs Lactobacilli, Enterocoquess, Leuconostoc sp, bacteriocins activities, indicators microorganisms, Listeria innovii growth media, pH, temperature
   
Résumé
   The objective of this study was to isolate LAB from dairy, meat products and agro-industrial wastes and to investigate their antagonist activity. A total of 141 isolates were screened for the inhibitory effect on ten indicator strains in the agar spot test. Results showed that strain LBbb0141 contained antimicrobial compound with wide spectrum that inhibited the growth of ten indicator Gram-positive and Gram-negative strains. The bacteriocins activity attained its maximum value using the MRS agar at initial pH 7.5 and 30°C incubation temperatur
Article 51 :Année de publication 2012
Titre Screening of autochthonous Lactobacillus species from Algerian raw goats’ milk for the production of bacteriocin-like compounds against Staphylococcus aureus
   
Revue, référence African Journal of Microbiology Research , African Journal of Microbiology Research Vol. 6(12), pp. 2888-2898, 30 March, 2012
   
Autres auteurs Mami Anas
Boumehira Ali Zinedine
Hamedi Amine Rizk
Henni Jamal Eddine
Kihal Mebrouk
   
Mots Clefs Raw goats’ milk, lactic acid bacteria, Lactobacillus plantarum, interaction, bacteriocin, Staphylococcus, mixed culture.
   
Résumé
   Lactic acid bacteria play a key role in maintaining the balance of normal gastro-intestinal micro flora. Food contamination by Staphylococcus aureus is a major problem for consumer’s health in Algeria, especially during the summer period. The use of bacterial interactions is a new way to limit the pathogenic germs growth. Detection of antimicrobial substances produced by lactic acid bacteria against the undesirable germs is the objective of this work. Microbiological and biochemical methods were used to identify lactic acid bacteria having an antimicrobial activity. The 2 isolates of lactic acid bacteria obtained from raw goats’ milk in western Algeria's areas were identified: Lactobacillus rhamnosus, Lactobacillus plantarum. The interactions study revealed that three lactobacilli species: L. plantarum (58) and L. plantarum (68) are able to inhibit S. aureus’ growth. In mixed culture after 24 h, L. plantarum reduces the growth of S. aureus by 1.6 log and this latter bacteria was not found after 72 h. The various tests used reveal the proteinic nature of the substance which was responsible for the growth inhibition of S. aureus. The ecological adaptation and growth characteristics of cultures of L. plantarum in food products will determine their effectiveness as bio-control agent in dairy food
Article 50 :Année de publication 2012
Titre Characterization and Technological Properties of Bifidobacterium Strains Isolated from Breast-fed Infants
   
Revue, référence Journal of Food Science and Engineering, Journal of Food Science and Engineering 2 (2012) 576-582 http://www.davidpublishing.org/show.html?12006
   
Autres auteurs Bahloul Halima Auras
Hadadji Miloud
Guessas bettache
Saidi Noureddine Kihal Mebrouk
   
Mots Clefs Ecology, Bifidobacterium, antibiotic , resistance, kinetics of growth, acidity.
   
Résumé
   Bifidobacteria represent the largest group of human intestinal bacteria. They have an important place in human health and represent the dominant group microflora of the newborn breast-fed. Following the behavior of strains of Bifidobacteria isolated from the breast-fed infants and from saline rehydration solution was considered in order to develop therapeutic fermented milk. Samples from newborn infants aged 10 months, or from a saline rehydration solution (Celia/Develop ORS) containing Bifidobacteria sold was used and isolated strains belonged to breve and longum species. Those strains showed preferences to neutral pH. They are mesophilic and tolerate high temperatures (42 °C). Glucose was commonly carbohydrate used in selective media for Bifidobacteria. Production of titratable acidity and therefore lowering the pH varies from one strain to another
Article 49 :Année de publication 2012
Titre Selection of lactic acid bacteria producing antimicrobial strain such the genus lactococcus isolated from algerian raw goat’s milk
   
Revue, référence International Journal of Nutrition and Food Sciences, International Journal of Nutrition and Food Sciences 1(1) : 23-32 (http://www.sciencepublishinggroup.com/j/ijnfs)
   
Autres auteurs Benhamouche N. Talhi M. Kihal M.
   
Mots Clefs Bacteriocin , Listeria, St. Aureus, Goat's Milk; ADNR16s, PCR / RAPD
   
Résumé
   Lactic acid bacteria are known for their ability to produce inhibitory substances against unwanted germs involved in food poisoning such as Listeria innocua. Species of lactic acid bacteria can inhibit harmful germs subject of this work. techniques confrontation on solid medium and the effect of the substance on the growth of Listeria innocua were performed. The experience was conducted bacterial antagonism in the solid medium by the method of double layer and method of wells, the whole experience was packaged in a buffered medium in order to eliminate the effect of acidity . 7 strains that isolates gave an antagonistic action against Listeria innocua. The strain of Lactococcus lactis (8b), isolated from raw goat's milk showed an anti-listeria in vitro and was selected because of its ability to inhibit as Staphylococcus aureus. With a diameter of 15mm, the action of proteolytic enzymes, trypsin, chymotrypsin is shown that the substance was protein in nature, the kinetics of growth in milk medium showed that the number of St. aureus after 24 h of incubation was reduced to 7.68 log cfu in mixed culture with strain 8b which was comparable to the control of 9.14 log cfu, which signifies that the survival rate was 3.4%, the phenotypic (biochemical and physiological) and molecular-based ADNr16Ss showed that the strain is Enterococcus faecium, duran, hirae. The sequencing results showed that Leuconostoc mesenteroides and Enterococcus faecium have summers dominant anti-Listeria species in milk samples from Algerian goat. Isolates had the potential of multiple bacteriocin production and do not have some important virulence. Importance and impact of the study: The Enterococci in milk in this region of western Algeria could be partly responsible for the safety of cheese and could be useful for the production of anti-Listeria cultures protection. The purpose of the study: Our study's main objective is the selection ,and study phenotypic and molecular lactic acid bacteria from Lactococcus genus possessing biotech traits such as the production of new inhibitory substance and the study of the interaction of Listeria innocua screw opinion and St. aureus that both methods were used, a traditional approach based on morphological and biochemical studies of different cultural characteristics and a method of direct molecular amplification of bacterial DNA using PCR / RAPD colony on having targeted DNA fragment 16S lactic acid bacteria using the two primers universal: 20F (5'AGAGTTTGATCATGGCTCAG-3 '). 1500R (5'-GGTTACCTTGTTACGACTT-3 ') and two specific primers OPA-3 (5'-AGTCAGCCAC - 3') and OPH 3 (5'-AGACGTCCAC-3 ') (Bioprobe, France).
Article 48 :Année de publication 2011
Titre Antibiotic resistance of some lactobacilli isolated from the gut microflora of broiler
   
Revue, référence African Journal of Microbiology Research, Vol. 5(13), pp. 1707-1709, 4
   
Autres auteurs Behira Belkacem
Kihal Mebrouk
   
Mots Clefs Lactobacilli, r,. antibiotics, resistance, broile, microflora
   
Résumé
   To evaluate the impact of the antibiotic use in poultry and the development of the resistance in the gut microflora of broiler. Microbiological methods were used to investigate the susceptibility of ten lactobacilli stains isolated from digestive tract of broiler. The isolates were tested against the ten most used antibiotics in veterinary and human medicine in Algeria. The isolates exhibited high resistance to all antibiotics tested in the range 70 to 100%. The minimal inhibitory concentrations (MIC’s) also were evaluated for each isolates. Lactobacilli of broiler’s microflora are a reservoir of resistance genes able to dispread the antibioresistance phenomenon through the food chain and the environment. Further studies need to be performed to understand the mechanisms and the causes responsible for this phenomenon. This study shows the need to find alternatives and emergency measures to avoidrepercussions on public health.
Article 47 :Année de publication 2011
Titre CHARACTERIZATION OF LACTOBACILLUS FROM ALGERIAN GOAT’S MILK BASED ON PHENOTYPIC, 16S rDNA SEQUENCING AND THEIR TECHNOLOGICAL PROPERTIES
   
Revue, référence Brazilian Journal of Microbiology, 42: 158-171
   
Autres auteurs Ahmed Marroki1
Manuel Z??iga2
Mabrouk Kihal3
Gaspar Pérez
Mart?nez2
   
Mots Clefs Lactic acid bacteria, Lactobacillus, identification, goat’s milk, technological, properties, Algeria.
   
Résumé
   Nineteen strains of Lactobacillus isolated from goat’s milk from farms in north-west of Algeria were characterized. Isolates were identified by phenotypic, physiological and genotypic methods and some of their important technological properties were studied. Phenotypic characterization was carried out by studying physiological, morphological characteristics and carbohydrate fermentation patterns using API 50 CHL system. Isolates were also characterized by partial 16S rDNA sequencing. Results obtained with phenotypic methods were correlated with the genotypic characterization and 13 isolates were identified as L. plantarum, two isolates as L. rhamnosus and one isolate as L. fermentum. Three isolates identified as L. plantarum by phenotypic characterization were found to be L. pentosus by the genotypic method. A large diversity in technological properties (acid production in skim milk, exopolysaccharide production, aminopeptidase activity, antibacterial activity and antibiotic susceptibility) was observed. Based on these results, two strains of L. plantarum (LbMS16 and LbMS21) and one strain of L. rhamnosus (LbMF25) have been tentatively selected for use as starter cultures in the manufacture of artisanal fermented dairy products in Algeria.
Article 46 :Année de publication 2011
Titre Prevalence of mastitis infection in cattle in West Algeria and Identification of causing bacteria
   
Revue, référence Journal of Animal and Veterinary Advances, 10.22-3002-3005
   
Autres auteurs N. BENHAMED
M. MOULAY
H. AGGAD
J. E. HENNI
M. KIHAL
   
Mots Clefs Mastitis, Streptococcus, Staphylococcus, Enterobacteria, cow health, quality, hygiene, raw milk.
   
Résumé
   Mastitis is a common disease that decreases milk yield and quality in lactating cows. Because of higher treatment cost and higher culling rate, mastitis causes economical losses. The CMT test was used to detect infection of mastitis in the farms; the infection is detected in 55.16% of cattle herd. The responsible infections bacteria suspected mastitis were determined .The bacteriological analysis revealed the presence of varied dominant bacteria belonging to the species of Staphylococcus aureus (30.76%), Streptococcus sp. (30.76%) and Escherichia coli (23.07%). Theses bacterial species were responsible for the infection of the cow in the different samples .The milk acidity was variable, so that almost half of the samples were at pH (6.65 to 6.90). The establishment of a guide to monitoring and surveillance is essential for control of pathogenic bacteria.
Article 45 :Année de publication 2011
Titre Antibiotic Resistance of Some Lactobacilli Isolated from the Digestive Tract of Broiler in Western Algeria
   
Revue, référence Journal of Animal and Veterinary Advances, 10 | Issue: 14 | Page No.: 1859-1862
   
Autres auteurs Bahira Belkacem
Kihal Mebrouk
   
Mots Clefs Lactobacilli, antibiotics, resistance, broile, microflora
   
Résumé
   The lactobacilli of the digestive tract poultry are considered as a reservoir of genes for resistance to antibiotics. Lactobacilli colonizing the crop and intestines of broilers play an important role in maintaining the balance of the health of the animal. The current trend that aims to spread the use of lactobacilli as a probiotic in poultry is confronted by abuse as curative despite regulations and guidelines that prohibit the routine use of antibiotics for the treatment of microbial infections poultry. The study is conducted on the lactobacilli flora of broiler chickens in Western Algeria. Ten isolates from the crop and intestines broiler. Antimicrobial sensitivity test to ten different types of antibiotic were evaluated using the disc diffusion method. Inhibition zone diameter was measured of resistance or susceptibility
Article 44 :Année de publication 2011
Titre Identification and Characterization of Functional and Technological Lactobacillus plantarum Strains Isolated from Raw Goat and Camel Milk Collected in Algeria
   
Revue, référence JOURNAL OF PURE AND APPLIED MICROBIOLOGY, J. Pure & Appl. Microbiol., 5(2). 553-566
   
Autres auteurs Boumehira Ali Zineddine
Mami Anas
Hamedi Amine Rizk
Henni Jamal Eddine
Kihal Mebrouk
   
Mots Clefs Lactobacillus plantarum Milk, Poultry animals., Growth kinetics, Antagonism, Antibiotics, Probiotics
   
Résumé
   The role of lactic acid bacteria (LAB) in food preservation, prevention of poisoning, increased nutritional value and improved organoleptic quality of food, is indirectly related to food hungry from different continents and so the ensuring a sustainable development. This work identifies five strains Lb. plantarum, with an atypical growth at 45 °C. The results of technological test are satisfactory for industrial use. The strains are heat resistant, produce flavors and have a proteolytic activity. However, they do not produce dextran on MSE media. Strains of Lb. plantarum have a kinetics growth and acidification in MRS broth and skim milk at 30 °C, almost identical. But the strain Lb. johnsonii has a different kinetic growth and acidification. The best growth temperature for Lb. plantarum LbG22 strain was 37 °C. The strains Lb. plantarum LbC5 and LbC6 have the best antibacterial activity, they inhibited all tested strains. The Lb. plantarum LbG22 strain has the greatest inhibitory activity against the strains of Staphylococcus aureus, compared to other lactic acid strains studied. In mixed culture, growth of S. aureus ATCC 43300 was inhibited after 24h of culture with Lb. plantarum LbG22. Susceptibility to antibiotics was also evaluated with 33 antibiotics.
Article 43 :Année de publication 2010
Titre EFFICACITE COMPAREE DES ANTIGENES D’AGGLUTINATION RAPIDE DANS LE DIAGNOSTIC DE LA BRUCELLOSE ANIMALE EN ALGERIE
   
Revue, référence Rev. Microbiol. Ind. San et Environn., Vol 4, N°2, p : 69-82
   
Autres auteurs AGGAD Hebib AHMED AMMAR Yamina KIHAL Mabrouk
   
Mots Clefs antigènes, sérum, brucellose, efficacité, Algérie,
   
Résumé
   La brucellose demeure une préoccupation dans la plupart des pays en voie de développement alors ; son dépistage repose essentiellement sur l’épreuve à l’antigène tamponnée qui demeure le test le plus utilisé grâce à sa fiabilité, son utilisation aisée et son coût peu élevé. Nous avons étudié l’efficacité comparée de sept antigènes d’agglutination sur lame sur des sérums de vaches et de chèvres de statut brucellique indéterminé ainsi que ceux de vaches brucelliques. Les résultats ont été comparés au test de fixation du complément et le test de l’anneau. Cette épreuve qualitative a été ensuite transformée en épreuve quantitative en utilisant des dilutions croissantes du sérum et les résultats sont évalués. Les résultats généraux ont montré une grande différence relative entre ces antigènes et l’épreuve qualitative peut être transformée en test quantitatif dont les résultats sont définitifs lors de dilution élevée (1/16ème). La standardisation des tests permettrait d’éviter les grandes différences au niveau de leurs qualités et la lutte contre la brucellose humaine doit passer par un contrôle rigoureux et par l’éradication de cette pathologie chez les animaux domestiques.
Article 42 :Année de publication 2010
Titre Antimicrobial Resistance of Escherichia coli Isolated from Chickens with Colibacillosis
   
Revue, référence Global Veterinaria, 4 (3): 303-306
   
Autres auteurs AGGAD Hebib AHMED AMMAR Yamina KIHAL Mabrouk Hammoudi A
   
Mots Clefs Escherichia coli, Resistance, Antibiogram, Serotype, Poultry,
   
Résumé
   In order to asses susceptibility of Escherichia coli to seven antimicrobial drugs, we tested one hundred Escherichia coli isolates, recovered from broilers with clinical signs and lesions of colibacillosis in the West area of Algeria. Serogrouping showed that 52% of the isolates belong to one of the serotypes O78, O2 and O1. Antibiograms revealed a high level of resistance to oxytetracycline, trimethoprim-sulfmethoxazole and enrofloxacin (87, 70 and 45%, respectively). A low percentage of strains were resistant to gentamycin and nitrofurane (3 and 2%, respectively). All strains were susceptible to ampicillin. 72% of the isolates were resistant to at least 2 antibiotic and 45% were resistant to at least 3 antibiotics.
Article 41 :Année de publication 2010
Titre Synthesis and Antimicrobial Evaluation of 1,3,4-Oxadiazole-2-Thione from some Pyridine carboxylic Acids
   
Revue, référence Asian Journal of Chemistry, 22, 7 : 5535-5542
   
Autres auteurs Benhammadi S Adil Ali othmane Derdour Aicha Mami Anas kihal mabrouk
   
Mots Clefs 5-pyridyl, Oxadiazole, Antibacterial activity, thione,
   
Résumé
   The reaction of either 1,3,4-oxadiazole-5-thione 4 or 4-amino-1,2,4-triazole-5-thione 5 with phenacyl bromide resulted in the formation of 1,2,4-triazolo[3, 4-b]-1,3,4-thiadiazine derivative 8. In this study a series ofnew1-(2-aryl-5-phenethyl-1,3,4-oxadiazol-3(2H)-yl)ethanones 2a-e was synthesized by the cyclization of imines 1a-e using acetic anhydride. The products were evaluated for anti-bacterial and anti-fungal activity. Among the newly synthesized compounds, 1-(2-(4-(dimethylamino)phenyl)-5-phenethyl-1,3,4-oxadiazol-3(2H)-yl)ethanone (2a)and1-(2-(4-chlorophenyl)-5-phenethyl-1,3,4-oxadiazol-3(2H)-yl)ethanone (2b)were found to possess maximum activity against the tested strains of S. aureus and P. aeruginosa. It was concluded that para-substitution enhances the activity of synthesized oxadiazoles
Article 40 :Année de publication 2010
Titre Effet inhibiteur des especes de lactobacillus isolees du lait cru de chevre, sur la croissance de staphylococcus aureus
   
Revue, référence CRASTRA, 7, 3 :1-10
   
Autres auteurs Anas MAMI
Bettache GUESSAS
HENNI Jamal Eddine
KIHAL Mabrouk
   
Mots Clefs lait cru de chèvre, bactéries lactiques, interaction, bactériocine, Staphylococcus,
   
Résumé
Article 39 :Année de publication 2010
Titre Prevalence of Escherichia coli Enterohemorragic O157:H7 in frozen bovine meat in Algeria
   
Revue, référence World Journal of Dairy & Food Sciences, 5, 2: 183-185
   
Autres auteurs Mohamed Salih 1Barka
Mebrouk 2Kihal
   
Mots Clefs Escherichia coli O157:H7, dentification, serotype, frozen bovine meat, Algeria.
   
Résumé
   The presence of Escherichia coli O157: H7 in frozen imported meat was studied in north-western Algeria. Two hundred and fifty one samples were tested for the presence of E. coli serogroup O157: H7 using the standard method of culture and serotyping technique. The pathogen was detected in 0.44% of samples. The isolate strain was nonsorbitol fermenter, O157 and H7 agglutinating. It was tested using the method of KIRBY and BAUER according to the nccls standards to view resistance to antibiotics and was considered sensitive to the ten antimicrobial agents (ampicillin, chloramphenicol, gentamycin, colistin, flumequine, enrofloxacin, nitrofurantoin, cephalothin, trimethoprim-sulfamethoxazole and tetracycline). This study confirms that frozen imported meat is an important reservoir of E. coli O157:H7 pathogenic for human.
Article 38 :Année de publication 2010
Titre Activité Anti-Bactérienne de Lactobacillus plantarum isolée du lait crude chèvre d’Algérie vis à vis de Staphylococcus aureus
   
Revue, référence LES TECHNOLOGIES DE LABORATOIRE -,, Volume 5, N°21 .26-34
   
Autres auteurs MAMI ANAS
HAMEDI AMINE RIZK
HENNI JAMAL EDDINE
KERFOUF AHMED
KIHAL MEBROUK
   
Mots Clefs lait cru de chèvre, bactéries lactiques, interaction, bactériocine, Staphylococcus aureus, culture mixte
   
Résumé
   La contamination des aliments est un problème majeur pour le consommateur surtout durant la période estivale dans les pays méditerranéens L’exploitation des interactions bactériennes est un nouveau moyen pour lutter contre les germes indésirables. L’objectif de ce travail est la recherche des bactéries lactiques productrices de substances antimicrobiennes vis-à-vis des germes nuisibles. Les méthodes microbiologiques et biochimiques ont été utilisées pour identifier les bactéries présentant une activité antimicrobienne. Neuf isolats de bactéries lactiques ont été identifiés à partir du lait cru de chèvre dans les régions de l’Ouest algérien. Les espèces dominantes appartenant au genre Lactobacilles sont : Lb. rhamnosus, Lb. plantarum, Lb. casei, Lb. paracasei subsp. paracasei, Lb. acidophilus,Lb. delbrueckii subsp lactis, Lb. fermentum, Lb. paraplantarum et Lactobacillus sakei subsp. sakei. L’étude des interactions a révélé la capacité de trois espèces Lb. plantarum (58), Lb. Paracasei subsp. paracasei (55) et Lb. rhamnosus (68) à inhiber Staphylococcus aureus. En culture mixte, Lb. plantarum réduit considérablement la croissance de Staphylococcus aureus de 1,6 log après 12 h d’incubation et aucune croissance n’a été observée après 72h. Les différents tests utilisés révèlent la nature protéique de cette substance impliquée dans l’inhibition de Staphylococcus aureus.
Article 37 :Année de publication 2010
Titre Prevalence of Escherichia Coli Enterohemorragic O157:h7 in Frozen Bovine Meat in Algeria
   
Revue, référence Journal of Applied Sciences Research, 6(11): 1576-1580,
   
Autres auteurs Mohamed Salih Barka
Mebrouk Kihal
   
Mots Clefs Escherichia coli O157:H7, dentification, serotype, frozen bovine meat, Algeria.
   
Résumé
   The presence of Escherichia coli O157: H7 in frozen imported meat was studied in northwestern Algeria. Two hundred and fifty one samples were tested for the presence of E. coli serogroup O157: H7 using the standard method of culture and serotyping technique. The pathogen was detected in 0.44% of samples. The isolate strain was nonsorbitol fermenter, O157 and H7 agglutinating. It was tested using the method of KIRBY and BAUER according to the nccls standards to view resistance to antibiotics and was considered sensitive to the ten antimicrobial agents (ampicillin, chloramphenicol, gentamycin, colistin, flumequine, enrofloxacin, nitrofurantoin, cephalothin, trimethoprim-sulfamethoxazole and tetracycline). This study confirms that frozen imported meat is an important reservoir of E. coli O157:H7 pathogenic for human.
Article 36 :Année de publication 2010
Titre Biological control of vascular wilt disease of tomato caused by Fusarium oxysporum f.sp lycopersici by Pseudomonas fluorescens, Bacillus cereus, Serratia marcescens and Trichoderma harzianium
   
Revue, référence Research Journal of Agronomy J, 4.2.31-32
   
Autres auteurs Nour Eddine KARKACHI
Samia GHARBI2
Mebrouk KIHAL1
Jamal Eddine HENNI
   
Mots Clefs Fusarium, Biological control, Pseudomonas, Bacillus cereus, Trichoderma, Antagonisms inhibition
   
Résumé
   Evaluation of the antagonistic activity of three bacterial and a fungi with direct confrontation method and the filtrates culture against the growth of Fusarium oxysporum f.sp lycopersici showed the inhibition of the mycelia growth of Fusarium oxysporum f.sp lycopersici with Bacillus cereus, energized the low activity, and it was more significant with Serratia marcescens and Trichoderma harzianium for the 2nd day but with Pseudomonas fluorescens, it’s was for the 5th day . The filtrates of culture of these antagonists showed that only Serratia marcescens and Trichoderma have a rate of inhibition which varies between (40-95%), and of (20-30%) with Pseudomonas fluorescens
Article 35 :Année de publication 2009
Titre Atypical citrate-fermenting Lactococcus lactis strains isolated from dromedary's milk.‏.
   
Revue, référence Journal of Applied Microbiology, .1365-2672.2009.04459.x
   
Autres auteurs Drici H. Gilbert C
Kihal M Atlan D
   
Mots Clefs CitP, citrate fermentation, Lactococcus lactis ssp. lactis, biovar diacetylactis, thermotolerance,
   
Résumé
   Algerian dromedary's milk was screened for proteolytic isolates able to grow rapidly on agar milk medium. PCR experiments revealed that 74 proteolytic isolates belonged to the genus Lactococcus and harboured the prtP gene encoding the lactococcal cell-surface proteinase. Among these, 85% were able to ferment citrate (Cit+ phenotype) and were classified as Lactococcus lactis ssp. lactis biovar. diacetylactis. This classification was confirmed after sequencing of the 16S rDNA gene of five Cit+ isolates. In contrast to dairy lactococci described in the literature, several Cit+ isolates exhibited a tolerance to 50°C (Ther+) and alkaline pH. Two genetic approaches allowed to show the presence of four independent plasmids (so-called pTher, pPrt, pLac, pCit) associated with the four respective phenotypes: Ther+, cell-surface proteinase activity PrtP (PrtP+), lactose catabolism (Lac+) and citrate utilization (Cit+). Two types of pCit plasmid were amplified by inverse PCR: class 1 was characterized by a 9-kb plasmid harbouring the expected lactococcal citQRP operon and class 2 by a 23-kb plasmid harbouring the Leuconostoc cit cluster (citI-CitMCDEFGRP
Article 34 :Année de publication 2009
Titre Phenotypic and Whole Cell Protein Analysis by SDS-PAGE for Identification of Dominants Lactic Acid Bacteria Isolated from Algerian Raw Milk.
   
Revue, référence World Journal of Dairy & Food Sciences, 4 (1): 78-87
   
Autres auteurs Ghazi Fatima
HenniDjamal Eddine
Benmechernene Zineb
Kihal Mebrouk
   
Mots Clefs Lactic acid bacteria, identification, phenotypic method, SDS-PAGE, raw milk, Leuconostoc,
   
Résumé
   Phenotypic identification of 21 representative strains revealed the presence of the following species: Leuconostoc mesenteroides subsp. dextranicum, Weissella paramesenteroides, Lactococcus lactis biovar. diacetylactis, Pediococcus acidilactici, Enterococcus faecium, Lactobacillus rhamnosus, Lactobacillus paracasei subsp. paracasei and Lactobacillus plantarum. In order to validate the previous results, whole cell protein patterns were obtained using Sodium Dodecyl Sulphate Gel Electrophoresis (SDS-PAGE) of these lactic acid bacteria strains, were analysed by calculating the coefficients of similarity (>100) for each two strains which were 80.7% and 78% for next couple of strains (6, 13) and (43, L4) that were identified phenotypically as Weissella paramesenteroides and Leuconostoc mesenteroides subsp. dextranicum, respectively. The coefficients of similarity (>100) between one strain of Leuconostoc mesenteroides and one strain of Weissella paramesenteroides were 36%, 48%, 44.4%, 48.6%, 44.4% and 48.6%, for the following couples (L4, 13), (L4, 6), (43,13), (43,6), (27,13) and (27, 6), respectively, in agreement with distant phylogenetic relationship between Leuconostoc mesenteroides and Weissella paramesenteroides. The SDS-PAGE method allowed clarifying some ambiguous points in phenotypic identification. It had corroborate, complete and correct phenotypic identification, although, further study is required to accurate it.
Article 33 :Année de publication 2009
Titre Evaluation de la qualité hygiénique du lait dans l’ouest algérien
   
Revue, référence Revue Méd. Vét. / Revue de Médecine Vétérinaire, 160, 12, 590-595
   
Autres auteurs AGGAD H MAHOUZ F AHMED AMMAR Y KIHAL M
   
Mots Clefs Lait, bactériologie, mammites, qualité, hygiène résidus,
   
Résumé
   Des échantillons de lait ont été analysés en vue d’évaluer leur qualité hygiénique. Les germes prévus par la norme nationale ont été recherchés, des analyses physico-chimiques ont été menées en parallèle ainsi qu’une appréciation des cellules somatiques. La flore mésophile totale était très élevée au niveau du lait cru de mélange (83.104 ufc/ml). Les streptocoques et les coliformes fécaux, absents dans les laits pasteurisés, étaient plus nombreux au niveau du lait de mélange (64 ufc/ml et 9,7 ufc/ml respectivement) ; la teneur en coliformes fécaux était cependant acceptable ainsi que celle des clostridies. Staphylococcus aureus se trouvait en plus grand nombre dans le lait cru. Les mammites sont décelées dans 76,47 % des laits de mélange et 47,17 % des laits individuels. Des résidus antibactériens sont décelés dans 29 % des échantillons. L’acidité du lait était variable alors que la moitié des échantillons avaient une densité inférieure à la normale. Des écarts-types importants indiquent une qualité irrégulière de ce produit, en relation avec une conscience incomplète de l’importance de l’hygiène. La maîtrise des bactéries pathogènes et des résidus dans le lait nécessite la mise en place de systèmes de contrôle et de surveillance.
Article 32 :Année de publication 2009
Titre Probiotic Potential of Thermotolerants Lactobacilli Isolated from Chicken Gastrointestinal Digestive and Their Use as Poultry Feed
   
Revue, référence World Applied Sciences Journal, 7 (8): 951-957
   
Autres auteurs Behira Belkacem Moulay Meriem Kihal Mebrouk
   
Mots Clefs Thermotolerant % % %, Poultry, Probiotic, Lactobacilli, Gallus domessticus,
   
Résumé
   This research was conducted in order to evaluate probiotic properties of lactobacilli species isolated from intestinal tract of indigenous Algerian chickens. Thirteen isolates thermotolerant lactobacilli were isolated from the crops and small intestines of domestic checkens (Gallus domessticus). The probiotic are a live microbial feed supplements which positively affects the health of the host animal by improving its intestinal balance. These isolates were characterized and identified. They were screened by use main criteria’s of probiotic selection in vitro, only the isolate survived and grown in acidic medium and resisted to bile salts were retained. The results showed that among thirteen isolates five isolates were responded to these criteria and were identified as Lactobacillus gallinarum, Lactobacillus crispatus, Lactobacillus dulbreukii, Lactobacillus acidophilus and Lactobacillus jonhsonni. These strains were homofermentatives and produced more than 0.6 % lactic acid (w/v) after 24 h of incubation and the nature of the isomeres were confirmed by the High Performance Liquid Chromatographic. All the strains were proteolytic. An anti-salmonella activity was detected in vitro against the species of Salmonella enteriditis and Salmonella infantis.
Article 31 :Année de publication 2009
Titre Carbon dioxide production by Leuconostoc mesenteroîdes grown in single and mixed culture with Lactococcus lactis in skim milk
   
Revue, référence Scientific Research and Essay, Vol. 4 (11), pp. 1348-1353,
   
Autres auteurs Kihal M Prevost H HenniD. E Benmechernene Z Diviès C
   
Mots Clefs Leuconostoc mesenteroîdes, fermented milk, Lactococcus lactis, Lactic; acetic and citric acid, milk, kinetics growth,
   
Résumé
   The effect of mixed culture of Leuconostoc mesenteroîdes subsp. dextranicum and Lactococcus lactis subsp. lactis (prot+) was investigated to achieve an optimal production of carbon dioxide. Only the strain of L. mesenteroîdes can produce carbon dioxide from lactose and citrate in milk. The influence of the initial concentration ration between the two strains on growth, carbon dioxide, L-lactate, acetic acid production and citrate used was studied. When the initial inoculum of L. lactis was 2.5 x 105 cfu/ml, the growth and evolved CO2 by L. mesenteroîdes (3 x 107 cfu/ml) increased, whereas high inoculum of L. lactis induced a decrease of growth and CO2 production by L. mesenteroides. In mixed culture, CO2 production continued after growth stopped, a partial uncoupling can be observed between growth and CO2 production. A shift of acetate production was observed in mixed culture and 25.6 mM was obtained, whereas 30.18 mM was obtained at the same time in pure culture of L. mesenteroîdes.
Article 30 :Année de publication 2008
Titre Evaluation de la qualité sanitaire des eaux brutes de consommation : cas de la ville de Béni-Abbès du sud ouest algérien.
   
Revue, référence Géographie et Aménagement, Bulletin, A.G.A.T, 13 : 67-77..
   
Autres auteurs Bengarnia Benmerine
Henni Jamal Eddine
Al Aboudi Abdul Kadhum
   
Mots Clefs Zones arides, eau de consommation, qualité de l’eau, Foggara, puits, Béni-Abbès
   
Résumé
   Le développement de la ville de Béni-Abbès, sous le poids de la démographie et des activités administratives et commerciales, a entraîné le détournement des eaux réservées à l’irrigation des sols de la palmeraie. L’analyse physico-chimique et microbiologique des ressources d’eau Ain Sidi Othmane, des deux puits (communal, forage1) et les anciennes foggaras ont fait l’objet de cette étude. Les résultats montrent en évidence que la source principale de Ain Sidi Othmane reste une source eaux de qualité sanitaire et hygiénique irréprochables avec un taux de résidus secs le plus faible. Les nouveaux puits communal et forage 1 présentent aussi une eau contenant un taux faible de sel par rapport à celui cité dans la bibliographie. En revanche les eaux de toutes les Foggaras sont inaptes à la consommation humaine par la présence de Clostridium. Par ailleurs, la foggara 1 contient la concentration la plus élevée en résidus secs qui est de 2,325 g/l. L’absence de germes dans la foggara 10 nous nous incite à approfondir les analyses physico-chimiques. Les sources destinées aux réseaux de distribution doivent être surveillées.
Article 29 :Année de publication 2008
Titre Kinetic of carbon dioxide production by Leuconostoc mesenteroides in single and mixed culture with Lactocccus lactis in skimmed milk.
   
Revue, référence Research Journal of Dairy Sciences, 2 (3): 30-35-.
   
Autres auteurs Prevost H
Henni DE.
Benmechernene Z.
Diviès C.
   
Mots Clefs Leuconostoc mesenteroides, Lactococcus lactis, milk, lactic acid, acetic acid, citric acid, growth, kinetics, fermented milk
   
Résumé
   The effect of mixed culture of Leuconostoc mesenteroides subsp. dextranicum and Lactococcus lactis subsp. lactis (prot+) was investigated to achieve an optimal production of carbone dioxide. Only the strain of Leuconostoc mesentroides can produce carbon dioxide from lactose and citrate in milk. The influence of the initial concentration ration between the 2 strains on growth, carbon dioxide, L-lactate, acetic acid production and citrate used was displayed. When the initial inoculum of Lactococcus lactis was 2.5 105 cfu mLG1, the growth and evolved CO by Leuconostoc mesenteroides (3 10 cfu mLG ) increased. Whereas, high inoculum 2 7 1 of Lactococcus lactis induce a decrease of growth and CO production by Leuconostoc mesenteroides. In 2 mixed culture CO production continued after growth stopped, a partial uncoupling can be observed between 2 growth and CO production. A shift of acetate production was observed in mixed culture and 25.6 mM was 2 obtained, whereas 30.18 mM was obtained at the same time in pure culture of Leuconostoc mesenteroides.
Article 28 :Année de publication 2008
Titre Antimicrobial activity of Lactobacillus species isolated from Algerian raw goat’s milk against Staphylococcus aureus.
   
Revue, référence World Journal of Dairy & Food Sciences, 3 (2): 39-49..
   
Autres auteurs Mami Anas
Henni Jamal Eddine
   
Mots Clefs Raw goats’ milk, Lactic acid bacteria, Lactobacillus plantarum, Interaction, Bacteriocin, Staphylococcus, Mixed culture
   
Résumé
   Food contamination by Staphylococcus aureus is a major problem for consumer’s health in Algeria, especially during the summer period. The use of bacterial interactions is a new way to limit the pathogenic germs growth. Detection of antimicrobial substances produced by lactic acid bacteria against the undesirable germs is the objective of this work. Microbiological and biochemical methods were used to identify lactic acid bacteria having an antimicrobial activity. The 08 isolates of lactic acid bacteria obtained from raw goats’ milk n western Algeria's areas were identified. The dominant species belonging to the Lactobacillus genera are: Lb. rhamnosus, Lb. plantarum, Lb. casei, Lb. paracasei subsp. paracasei, Lb. acidophilus, Lb. delbrueckii subsp lactis, Lb. fermentum, Lactobacillus paraplantarum and Lactobacillus sakei subsp. sakei. The interactions study revealed that three lactobacilli species: Lb. plantarum (58), Lb. paracasei subsp. Paracasei (55) and Lb. rhamnosus (68) are able to inhibit Staphylococcus aureus’ growth. In mixed culture after 24 h, Lb. plantarum reduces the growth of Staphylococcus aureus by 1.6 log and this latter bacteria was not found after 72h. The various tests used reveal the proteinic nature of the substance which was responsible of the growth inhibition of Staphylococcus aureus. The ecological adaptation and growth characteristics of cultures of Lb. plantarum in food product will determine their effectiveness as biocontrol agent in dairy foods.
Article 27 :Année de publication 2008
Titre Effect of pea cultivarm pathogen isolate inoculum concentration and leaf wetness duration on ascochyta blight caused by Mycosphaerella pinodes
   
Revue, référence Phytopathol. Mediterr. / Phytopathology Mediterraneen, 47. 214-222.
   
Autres auteurs Setti Benali
Bencheikh Mohamed
Henni Jamal
Neema Claire
   
Mots Clefs Disease severity, Incubation period, Pisium sativum
   
Résumé
   The effect of host leaf wetness duration Mycosphaerella pinodes inoculum concentration and pathogen isolate on the latent period and the inoculum period of the pathogen or deases severity were quatified on pea Pisium sativum…..
Article 26 :Année de publication 2008
Titre Comparative aggression of Mycosphaerella pinodes on peas fro; different regions in western Algeria
   
Revue, référence Phytopathol. Mediterr. / Phytopathology Mediterraneen, 48. 00-00.
   
Autres auteurs Setti Benali
Bencheikh Mohamed
Henni Jamal
Neema Claire
   
Mots Clefs Disease severity, Incubation period, Pisium sativum, HCA PCQ, Aggressivity group
   
Résumé
   Mycosphaerella blight caused Mycosphaerella pinodes deases severity were quatified on pea Pisium sativum…..
Article 25 :Année de publication 2007
Titre carbon dioxide production by Leuconostoc mesenteroides in single and mixed culture with Lactocccus lactis in skimmed milk.
   
Revue, référence World Journal of Dairy & Food Sciences, 2 (2): 62-68.
   
Autres auteurs Prevost H.
Henni DE.
Benmechernene Z.
Diviès C
   
Mots Clefs Leuconostoc mesenteroides, Lactococcus lactis, milk,, lactic acid , acetic acid, citric acid, growth kinetics, fermented milk
   
Résumé
   The effect of mixed culture of Leuconostoc mesenteroides subsp. dextranicum and Lactococcus lactis subsp. lactis (prot+) was investigated to achieve an optimal production of carbone dioxide. Only the strain of Leuconostoc mesentroides can produce carbon dioxide from lactose and citrate in milk. The influence of the initial concentration ration between the 2 strains on growth, carbon dioxide, L-lactate, acetic acid production and citrate used was displayed. When the initial inoculum of Lactococcus lactis was 2.5 105 cfu mLG1, the growth and evolved CO by Leuconostoc mesenteroides (3 10 cfu mLG ) increased. Whereas, high inoculum 2 7 1 of Lactococcus lactis induce a decrease of growth and CO production by Leuconostoc mesenteroides. In 2 mixed culture CO production continued after growth stopped, a partial uncoupling can be observed between 2 growth and CO production. A shift of acetate production was observed in mixed culture and 25.6 mM was 2 obtained, whereas 30.18 mM was obtained at the same time in pure culture of Leuconostoc mesenteroides.
Article 24 :Année de publication 2007
Titre Synthesis and Antibacterial Activity of 1,3,4-Oxadiazole and 1,2,4-Triazole Derivatives of Salicylic Acid and its Synthetic Intermediates
   
Revue, référence S. Afr. J. Chem. / South African Journal of Chemistry, 60, 20–24,.
   
Autres auteurs Zoulikha Khiati
Adil A. Othman1
Bettache Guessas2
   
Mots Clefs 1,3,4-Oxadiazole-2-thione, 1H-1,2,4-triazole-5-thiol, 4-amino-1,2,4-triazole-5-thiol, salicylic acid, antibacterial activity
   
Résumé
   Eight compounds 2–9 have been synthesized starting from salicylic acid, two of them (7 and 9) are novel. The four final products namely: 5-(2-hydroxy phenyl)-1,3,4-oxadiazole-2-thione 4, 3-(2-hydroxy phenyl)-1H-1,2,4-triazole-5-thiol 6, 3-(2-hydroxy phenyl)-4-amino-1,2,4-triazole-5-thiol 8 and 3-(2-hydroxy phenyl)-1-amino-1,2,4-triazole-5-thiol 9 have been prepared using known reactions. The structures of intermediates and final products were determined by spectroscopic IR, UV, 1H-NMR & MS-methods in addition to elemental analysis. Antibacterial activities of compounds 1–6 and 8 were investigated in vitro against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa and the results are reported herein.
Article 23 :Année de publication 2007
Titre Caractérisation phenotypique of lactic acid bacteria isolées from believed milk of goat of two caprine populations Local Arabia and Kabile...
   
Revue, référence Journal of Animal and Veterinary Advances, 6 (12) : 1474-1481.
   
Autres auteurs Laouabdia N.S
Badis A
Guetarni J
Ouzroute R.
   
Mots Clefs Charactyerisation phenotypique, Lactic acid bacteria, Lactic acid , Goat's milk
   
Résumé
Article 22 :Année de publication 2006
Titre Phenotypic identification and whole cell protein analysis by sds-page of dominants lactic acid bacteria isolated from Algerian raw milk.
   
Revue, référence Journal Algerien des Zones Arides, 5 : 25-35..
   
Autres auteurs GHAZI F.Z.
BENMECHERNENE Z.
AGGAD H.
GUESSAS B.
MOUSSA
BOUDJAMAA
D. HENNI
   
Mots Clefs Lactic acid bacteria, identification, phenotypic method, SDS-PAGE, raw milk, Leuconostoc
   
Résumé
   Phenotypic identification of 21 representative strains revealed the presence of the following species: Leuconostoc mesenteroides subsp. dextranicum, Weissella paramesenteroides, Lactococcus lactis biovar. diacetylactis, Pediococcus acidilactici, Enterococcus faecium, Lactobacillus rhamnosus, Lactobacillus paracasei subsp. paracasei and Lactobacillus plantarum. In order to validate the previous results, whole cell protein patterns were obtained using Sodium Dodecyl Sulphate Gel Electrophoresis (SDS-PAGE) of these lactic acid bacteria strains, were analysed by calculating the coefficients of similarity (>100) for each two strains which were 80.7% and 78% for next couple of strains (6, 13) and (43, L4) that were identified phenotypically as Weissella paramesenteroides and Leuconostoc mesenteroides subsp. dextranicum, respectively. The coefficients of similarity (>100) between one strain of Leuconostoc mesenteroides and one strain of Weissella paramesenteroides were 36%, 48%, 44.4%, 48.6%, 44.4% and 48.6%, for the following couples (L4, 13), (L4, 6), (43,13), (43,6), (27,13) and (27, 6), respectively, in agreement with distant phylogenetic relationship between Leuconostoc mesenteroides and Weissella paramesenteroides. The SDS-PAGE method allowed clarifying some ambiguous points in phenotypic identification. It had corroborate, complete and correct phenotypic identification, although, further study is required to accurate it.
Article 21 :Année de publication 2006
Titre A new manometric method for measuring carbon dioxide production by dairy starter cultures, a case of Leuconostoc mesenteroides.,
   
Revue, référence Afr. J. Biotechnol / African Journal of Biotechnology, 5, (4) : 378-383.
   
Autres auteurs Henni DE
Prévost H.
Diviès C
   
Mots Clefs Leuconostoc sp, CO2 production, lactic acid, milk
   
Résumé
   This paper describe a new technique for carbon dioxide (CO2) measurement based on the displacement of acidifying liquid in burette at constant pressure. It was adapted to allow the growth of Leuconostoc sp. On semi-synthetic media and milk. A highly precise measurement was obtained using the chemical reaction of sodium carbonate with hydrochloric acid. This method has been proven to present an excellent linear response from 0 to 0.895 mM of CO2 with a high sensitivity. With cultures in milk medium, kinetics of CO2 evolved by Leuconostoc mesenteroides subsp. dextranicum L4 were higher than L. mesenteroides subsp. mesenteroides 19D and L. mesenteroides subsp. cremoris Ec195 grown on milk.. The use of L. mesenteroides subsp. dextranicum L4 and their variants Lac(-)Cit(+), Lac(+)Cit(-) and Lac(-)Cit(-) showed that the CO2 production correlated to the substrate utilization. The curve of CO2 measured was close to the amount of CO2 calculated from lactose and citrate used. The CO2 production rate was higher with the parental strain (6.5 mM/ h) than with the variants. The amount of CO2 produced was always close to the theoritical value, and was also correlated to the growth rate and substrates utilization by L. mesenteroides. The volume of CO2 produced by Leuconostoc in milk was highly correlated with the D-lactate production (r = 0.995). This method could be used routinely for the evaluation and the selection of bacteria having potential ability for CO2 production.
Article 20 :Année de publication 2006
Titre Proteolytic activity of cultivable lactic acid bacteria isolated from Algerian raw goat’s milk.
   
Revue, référence World Journal of Dairy & Food Sciences, 1 (1): 12-18,.
   
Autres auteurs Moulay. M.
Aggad H.
Benmechernene Z.
Guessas B.
Henni D.E.
   
Mots Clefs Lactic acid bacteria, proteolysis, caseins, goat's milk
   
Résumé
   The proteolytic systems play an essential role in nitrogen metabolism of lactic acid bacteria in milk. The extracellular cell wall-bound proteinase is a key enzyme in this system, in which its activity is necessary for the growth of lactic acid bacteria in milk by initiating the breakdown of casein to smaller peptides. The screening of the proteolytic strains has been achieved on three solid media YMA, PCA and FSDA. The strain of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis, biovar. diacetylactis and the Enterococcus were tested. Lactococcus lactis produced a large hydrolysis zone more than 10 mm of diameter. The concentration of 3 and 5% of inoculum produced the highest acidity which was superior to 60°D. The proteolytic strains of Lactococcus lactis subsp. lactis biovar. diacetylactis at 3% of inoculum showed a maximal acidification rate 5°D /h and 0.3 unit pH/h. The strongest proteolytic strain Lactococcus lactis subsp. lactis biovar. diacetylactis produced an acidification rate of 8°D/h and a pH rate which decreases to 0.28 unit of pH/h with a final pH value of 4.29. The rate of casein hydrolysis by the original Lactococcus lactis subsp. lactis biovar. diacetylactis (90 mg hG1) was three times higher. The proteolytic activity of this strain can be exploited for the selection of performante lactic acid bacteria for the Algerian dairy industry.
Article 19 :Année de publication 2006
Titre Growth and lactic acid production by Bifidobacterium longum and Lactobacillus acidophilus in goat’s milk
   
Revue, référence Afr. J. Biotechnol / African Journal of Biotechnology, Vol. 5 (6), pp. 505-509, 15.
   
Autres auteurs Miloud Hadadji1
Ahmed Bensoltane
   
Mots Clefs Bifidobacterium longum, Lactobacillus acidophilus, fermented milk, growth, lactic acid
   
Résumé
   The fitness of a particular strains of Bifidobacteria, Lactobacilli and Streptococci for commercial utilization depends on its rapid growth and acidification of milk as well as its acid and oxygen tolerence. From 20 samples of French commercial yoghurt, one species of bifidobacteria was identified as Bifidobacterium longum. Also, Lactobacillus acidophilus was isolated and identified from fermented milk. The rate of growth was 0.37 h-1 on TPY medium and 0.18 h-1 in milk with Bifidobactrium longum. The production of lactic acid was compared with pure and mixed cultures and the values were 90 and 64 mM at 37°C; 82 and 140 mM at 45°C. The maximum rate of lactic acid production was obtained with mixed culture at 45°C.
Article 18 :Année de publication 2005
Titre Identification of cultivable bifidobacterium species isolated from breast-fed infants faeces and their technological properties for potential use as a probiotic cultures..
   
Revue, référence Afr. J. Biotechnol / African Journal of Biotechnology, 4 (5) : 422-430.
   
Autres auteurs Hadadji M
Benaama R.
Saidi N.
Henni D.
   
Mots Clefs Lactic acid bacteria, Bifidobacterium, probiotic, fermented milk, growth, survival
   
Résumé
   Gastrointestinal microflora exerts a high impact on its host mainly by performing a great variety of metabolic activities, protecting the host against colonization by pathogen and stimulating the gut immune system. The integration of bifidobacteria in dairy products is of interest for reinstalling the intestinal microflora. The mother's milk is a prebiotic factor which stimulate the bifidobacteria growth in vivo. Results obtained in west Algeria area show that the number of bifidobacteria reach 26 109 cfu/g in breast-fed infant feces. The microbiological, fructose–6-phospho-ketolase and the biochemical tests have revealed Bifidobacterium strains. The most prevalent isolates belong to the species Bifidobacterium longum (75%) and Bifidobacterium breve (25%). In order to evaluate the potential use as a starter culture, the occurrence of some selected properties such as growth and survival in fermented milk were investigated. These strains have a fast growth in glucose, galactose and lactose media compared to reference species. All isolates studied are sensitive to the pH 8. The B. breve strain support the conservation conditions in pure culture better than in mixed culture with Streptococcus thermophilus or Lactobacillus acidophilus.
Article 17 :Année de publication 2005
Titre Characterization of algrian raw camel’s milk identification of dominant lactic acid bacteria and proteins analysis.
   
Revue, référence Journal algérien des zones Arides, (4): 01-09..
   
Autres auteurs Saidi D
Kihal M.
Hamama A.
Chekroun A.
Henni DE.
Kheroua O
   
Mots Clefs Camel’s milk, Lactic Acid Bacteria, Proteins, Acidification
   
Résumé
   The chemical composition of Algerian raw camel’s milk is slightly rich in fat 34.4 +- 2.8 g.l-1, proteins 33.1 +- 2.1 g.l-1, lactose 45.1 +- 3.1 g.l-1, ash 8.15 +- 0.15 g.l-1 and total solids 122.6 +- 0.12 g.l-1. This composition varied by several factors such as feeding, breeds, milk yielding and the health of the animal. Our results showed that total casein proteins were higher than whey proteins. SDS-PAGE showed that casein proteins of camel’s milk and cows’ milk have the same molecular weight, about 24 kDa. The whey proteins of camel’s milk were presented by 5 bands. Compared with cow’s milk, camel’s milk contents a little amount of β-lactoglobuline. The pH of raw milk decrease from 6.5 to 5.4 after 72 h of incubation at 30°C. The total count of lactic acid bacteria can reach 200 x 106 cfu ml-1 in MRS medium. Fourty strains of lactic acid bacteria were isolated from camel milk. The mesophilic group were represented by Lactococcus lactis subsp. lactis biovar. diacetylactis which is the dominant strain, L. lactis subsp. Lactis, L. lactis subsp. cremoris and L. lactis subsp. lactis biovar. diacetylactis produce more than 100 mM of lactic acid after 24 h of incubation at 30°C. The thermophilic strains were represented by two species, Streptococcus thermophilus and Enterococcus faecalis. From a technological point of view, the camel milk can be used as a source of new starters in the development of the industrial manufacture of these strains
Article 16 :Année de publication 2005
Titre Inhibition of Staphylococcus aureus growth in milk by lactic acid bacteria
   
Revue, référence Dirasat, 32. 3 : 53-60.
   
Autres auteurs Guessas B.
Hadadji M.
Saidi N
   
Mots Clefs Drinking water, coliforms bacteria, foggara,Adrar, Algeria, arid zones,
   
Résumé
   From ancient times the arid zones of Sahara, south Algeria, stay in a good state without signified contamination with biological and chemical matters. At the last five decades, the zones show large movement of population, therefore that became a new source of microbial and chemical contamination. The microbiological quality of drinking water has been evaluated in 28 samples from underground wells and foggara water which were analyzed. The essential microflora : aerobic bacteria (29,33 ± 0,44) cfu per100 ml, total coliforms (6,6 ± 0,15) cfu per 100 ml, fecal coliforms (2,33 ± 0,42) cfu per 100 ml and fecal streptococci (1,51 ± 0,66) cfu per 100 ml. The pH ranged from 6,22 to 7,34. Likewise, in 100 % of drinking water more than 71,42 % was non drinking water.
Article 15 :Année de publication 2005
Titre Caractérisation phenotypique des bactéries lactiques isolées à partir de lait cru de chèvre de Deux populations caprines locales Arrabia et Kabyle.
   
Revue, référence Sci Technol. / Science & Technologie, 23 : 30-37.
   
Autres auteurs Badis A.
Laouabdia
Sellami N.
Guetarni D
Ouzrout R
   
Mots Clefs bactéries lactiques, lait cru, chèvre, caractérisation, phénotypique
   
Résumé
   La distribution qualitative des bactéries lactiques est étudiée dans le lait cru de chèvre de deux populations caprines algériennes (Kabyle et Arabia). Cette distribution semble montrer que le genre Lactobacillus est nettement prédominant dans la population Kabyle (61.48 %); suivi de, Lactococcus (22.9%), Streptococcus (8.88%), Leuconostoc (5.92%) et de loin de Pediococcus (0.74 %), alors que les deux genres Leuconostoc et Lactococcus sont représentés de façon presque similaire dans la population Arabia (32.64 % et 31.02 % respectivement), ainsi que Lactobacillus (15.27 %), Streptococcus (14.82 %) et Pediococcus avec une faible représentation (6.25 %). L'identification des 240 isolats selon les critères morphologiques, physiologiques et biochimiques a permis de les rattacher aux 28 espèces présumées dont les plus dominantes sont : Lactobacillus helveticus (29 isolats), Streptococcus thermophilus (23 isolats), Lactobacillus brevis (20 isolats), Lactobacillus caséi subsp caséi (20 isolats), Lactobacillus plantarum (16 isolats), Lactococcus lactis subsp. lactis (14 isolats) et Pediococcus .acidilactici (12 isolats ).
Article 14 :Année de publication 2004
Titre Identification and technological properties of lactic acid bacteria isolated from raw goats milk of four Algerian races..
   
Revue, référence Food Microbiol, 21. 5 : 579-588.
   
Autres auteurs Badis A.
Guetarni D.
moussa Boudjema B.
Henni DE
   
Mots Clefs Lactic acid bacteria, Raw milk, Goat, Technological, characteristics
   
Résumé
   The characteristics of725 lactic acid bacteria isolated from raw goat milk off our Algerian races were studied. They were phenotypically classified as Lactobacillus (31.6%), Lactococcus (28.4%), Leuconostoc (22.2%), Streptococcus (13.7%) and Pediococcus (4.1%). No major differences were observed as to the distribution of genus isolates in the milk of three races (Makatia, Makatia-Chamia and Kabyle). However, the number ofisolates ofboth Leuconostoc and Lactococcus predominated in Arabia milk while the samples collected from the other races mostly contained lactobacilli isolates. The phenotypic and biochemical analyses gave a diversity ofspecies (28 presumptive species). The most abundant species were Streptococcus thermophilus (46 isolates), Lactobacillus helveticus (45 isolates), Lactobacillus plantarum (30 isolates), Lactobacillus delbruecki subsp. bulgaricus (26 isolates), Lactobacillus brevis (25 isolates) and Lactococcus lactis subsp. lactis (25 isolates). The technological properties of the isolates were as follows: 38.6% of isolates showed a fast acidifying rate (more than 60_D during 18 h ofincubation); 25.9% ofisolates had a high proteolytic capacity (more than 6 ppm leucine/72 h) and 14.1% of isolates had a high diacetyl production (more than 0.8 ppm diacetyl/16 h). Nine isolates were selected to prepare yoghurt and cheese. Sensory tests performed by a trained panel revealed a pleasant yoghurt prepared with mixed culture St. thermophilus 16 TMC+L. helveticus 20TMC. Cheese prepared with isolates L. lactis subsp. Lactis biovar diacetylactis 19 MCA and L. lactis subsp. lactis 10 MCM had a very good sensory quality and a pleasant taste.
Article 13 :Année de publication 2004
Titre Characteristics of cultivable lactic acid bacteria isolated from Algerian raw goat’s milk and evaluation of their technological properties
   
Revue, référence Food Microbiol, 21. 3 : 343-349.
   
Autres auteurs Badis A.
Guetarni D.
moussa Boudjema B.
Henni DE
Tornadijo. E
   
Mots Clefs Lactic acid bacteria, Goat’s milk, Identification, Technological, characterization
   
Résumé
   One hundred and fifty-eight strains of lactic acid bacteria isolated from Algerian raw goat’s milk were identified and technologically characterized. Five genera were found: Lactobacillus (50.63%), Lactococcus (25.94%), Streptococcus (14.56%), Leuconostoc (7.59%) and Pediococcus (1.26%). The predominant species were Lactococcus lactis (32 strains), Streptococcus thermophilus (23 strains), Lactobacillus bulgaricus (19 strains), Lb. helveticus (16 strains) and Lb. plantarum (14 strains). Approximately 39% of the lactic acid bacteria isolated produced more than 0.6% lactic acid (w/v) after 18 h of incubation, and belonged to the Lactococcus and Lactobacillus genera. The highest proteolytic activity was approximately 3mg tyrosine l_1 for mesophilic strains and nearly 5mg tyrosine l_1 for thermophilic lactobacilli after 72 h. High aromatic activity (more than 0.8 mg diacetyl l_1 after 16 h) was detected in 14% of the strains. Nine strains were used to make dairy products (a yoghurt-like product and Edam-type cheese) on a pilot scale in the laboratory. The best-liked organoleptic characteristics were noted in a yoghurt produced with a mixed culture made up of S. thermophilus (strain 16TMC+) and Lb. helveticus (strain 20TMC) and in a cheese made with a starter composed of Lc. lactis subsp. lactis (strain 10MCM) and L. lactis subsp. lactis (V.P. +) (strain 19MCM).
Article 12 :Année de publication 2004
Titre Actividad antimicrobiana de bacterias lacticas sobre listeria monocytogenes.
   
Revue, référence Alimentaria, 355 : 11-16.
   
Autres auteurs Moussa Boudjemaa B.
Tarnadijo E
Bensoltane A.
Fresno J.M
   
Mots Clefs Lactic acid bacteria, listeria, interactions, inhibition,
   
Résumé
   A number of simple of raw milk produced in Tlemcen in northwerten algerai were investigated so as to detect Listeria monocytogenes. However, it was not possible to detect its presence in any of the simples analysis. The antibacterial activity of fifty strains of lactic acid bacteria was tested against three strains of listeria monocytogenes(SV1/2a, SV4b, and LO28). In each order to check the sensitivity od one strain relative to another the agar spot test was used. Local lactic acid bacteria showed anti listria activity could have a potential application to the field of food preservation
Article 11 :Année de publication 2004
Titre Characterization of lactic acid bacteria isolated from Algerian arid zone raw goats’milk..
   
Revue, référence Afr. J. Biotechnol / African Journal of Biotechnology, 3 (6) :339-342.
   
Autres auteurs guessas B
   
Mots Clefs Lactic acid bacteria, raw milk, goat, arid zones, identification
   
Résumé
   Diversity and density of lactic acid bacteria isolated from Algerian raw goats' milk in arid zones were studied by determination of morphological, cultural, physiological and biochemical characteristics. 206 lactic acid bacterial strains were isolated, with 115 of them belonging to lactic acid cocci and others to the genus, Lactobacillus. The representative species of the total cocci were Lactococcus sp. (76.16%), Streptococcus thermophilus (14.78%) and Leuconostoc sp. (8.6%). The dominating species is Lactococcus lactis subsp. Lactis. Lactobacilli species found in local raw goats' milk and their proportion were: Lb. curvatus (25.25%), Lb. helviticus (10.98%), Lb. plantarum (9.89%), Lb. reuteri (9.89%), Lb. casei (7.69%), Lb. brevis (5.49%), Lb. bulgaricus (5.49%) Lb. paracasei (4.39%) and Lb. acidophilus (2.19%).
Article 10 :Année de publication 2004
Titre Etude physiologique des bactéries lactiques isolées du lait cru de chèvre de la race Ouled Djellal
   
Revue, référence Journal algérien des zones Arides, 3. 15-19.
   
Autres auteurs Badis A.
GuetArni D
Naceur M.
   
Mots Clefs Bactéries lactique, Taxonomie, Lait, Chèvre
   
Résumé
   Le pressent travail est une contribution à l’étude physiologique des bactéries lactiques du lait cru de chèvre de la race locale « Ouled Djellal ». L’utilisation des milieux sélectifs a permis d’isoler un nombre élevé de bactéries lactiques. A partir de 432 isolats ont été identifiés suivant les critères phénotypiques, physiologiques et biochimiques et qui se repartissent en 5 genres dont Leuconostoc (32,6 % ) et Lactococcus (31,8 % ) nettement prédominants, suivi, de Lactobacillus (15,3 % ), Streptococcus (14,81 % ) et de loin Pediococcus (6,3% ) et 22 espèces.Parmi les espèces des bactéries lactiques les plus importantes numériquement, nous pouvons citer le Streptococcus thermophilus (64 isolats), Lactococcus plantarum (72 isolats), Lactococcus lactis.subsp lactis (45 isolats), Leuconostoc amylibiosum (48 isolats), Leuconostoc lactis (36isolats) et Leuconostoc mesentroides subsp. dextranicum (26 isolats).
Article 09 :Année de publication 2004
Titre The incidence of Bacillus cereus in Algerian raw milk : A study of the chief soureces of contamination.
   
Revue, référence Archiv Für Lebensmittelhygiene, 55. 93-96.
   
Autres auteurs Moussa Boudjemaa B.
Lopez M.
Gonzalez J.
   
Mots Clefs
   
Résumé
Article 08 :Année de publication 2003
Titre Contribution à l’étude de lactobacilles thermophiles à caractère probiotiques isolées du tractus digestif de la volaille..
   
Revue, référence MagVet, 46 :5-7..
   
Autres auteurs Bahira B
Bensalah F.
   
Mots Clefs
   
Résumé
Article 07 :Année de publication 2003
Titre Cartographie d’un plasmide pSU 100 isolé à partir de Lactobacillus casei isolé du lait cru d’Algérie.
   
Revue, référence Sci Technol. / Science & Technologie, 19 : 1004-110.
   
Autres auteurs Bensalah F
saidi N
   
Mots Clefs Lactobacillus casei, Plasmide, E.coli, clonage, Carte de restriction
   
Résumé
   Les bactéries isolées de la flore de lait cru local reste très peu connue quant à leur potentialité et performance génétique. L’intérêt des différentes propriétés biologiques portées par les plasmides de ces bactéries intéresse de nombreux laboratoires de recherche. Les techniques d’extraction et de purification nous ont permis de détecter un plasmide de 5.3 Kb chez Lactobacillus casei, bactérie isolé de lait de vache. Ce plasmide appelé pSu100 a été cloné dans le vecteur de transformation pUC18 au site EcoRI chez E.coli, JM103. les profils electrophoretiques de restriction obtenus par des digestions simples, double et triple sous l’action de 33 endonucléase ont contribué à l’élaboration d’une carte d’une restriction de ce plasmide. Cinq sites uniques ont été identifié ainsi que d’autres sites doubles et multiples. Une étude préliminaire du rôle physiologique de ce plasmide a permis de déceler une résistance a la Kanamycine.
Article 06 :Année de publication 2002
Titre Microbiological quality of drinking water at arid zones in Adrar South Algeria..
   
Revue, référence J. Alg. Reg. Arides / Journal Algérien des Régions Arides, 01 : 45-50..
   
Autres auteurs Boutiba Z.
Henni DE.
Al
Aboudi AK.
   
Mots Clefs Drinking water, coliforms, bacteria, foggara, Adrar, Algeria, arid zones.
   
Résumé
   From ancient times the arid zones of Sahara, south Algeria, stay in a good state without signified contamination with biological and chemical matters. At the last five decades, the zones show large movement of population, therefore that became a new source of microbial and chemical contamination. The microbiological quality of drinking water has been evaluated in 28 samples from underground wells and foggara water which were analyzed. The essential microflora : aerobic bacteria (29,33 ± 0,44) cfu per100 ml, total coliforms (6,6 ± 0,15) cfu per 100 ml, fecal coliforms (2,33 ± 0,42) cfu per 100 ml and fecal streptococci (1,51 ± 0,66) cfu per 100 ml. The pH ranged from 6,22 to 7,34. Likewise, in 100 % of drinking water more than 71,42 % was non drinking water.
Article 05 :Année de publication 2002
Titre Ethique de la recherche scientifique et les risques d’utilisation des armes de destruction massives اخلاقيات البحث العلمي والاخطار الناجمة عن استخدام اسلحة الدمار الشامل .
   
Revue, référence Arab. Journal of Sciences, 39 : 73-94.
   
Autres auteurs Abdul
kadhem A
   
Mots Clefs
   
Résumé
Article 04 :Année de publication 2002
Titre Phenotypic identification of dominant homofermentative lactic acid bacteria isolated from Algerian whole camels milk.
   
Revue, référence Selected Research Papers, 33 :10-16.
   
Autres auteurs Chekroun A.
Saidi D
Henni DE
Kheroua O
   
Mots Clefs Camels’ milk, lactic acid bacteria, proteins, growth, acidification, identification.
   
Résumé
   The chemical composition of Algerian raw camel’s milk is slightly rich in fat 3.44 ± 0.28%, proteins 3.31 ± 0.21%, lactose 4.51 ± 0.31, ash 0.815 ± 0.015% and total solids 12.26 ± 0.012%. This composition varied by several factors such as feeding, breeds, milk yielding and the health of the animal. Our results showed that total casein proteins were higher than whey proteins (57% and 43%) respectively. SDS-PAGE showed that casein proteins of camel’s milk and cow’s milk have the same molecular weight about 45 kDA. The whey proteins of camel’s milk were presented by 5 bands. The pH of raw milk decrease from 6.5 to 5.4 after 72h of incubation at 30°C. The total count of lactic acid bacteria can reach 200 x 106 cfu ml-1 in MRS medium. Forty strains of lactic acid bacteria were isolated from camel milk. The isolates were divided into two groups mesophilic and thermophilic. The mesophilic group was represented by Lactococcus sp., Lactococcus lactis subsp. lactis biovar. diacetylactis is the dominant strain and represent 60% of the total acid bacteria. Lactococcus lactis subsp lactis biovar. diacetylactis produces more than 100 mM of lactic acid after 24 h of incubation at 30°C. The termophilic strains were represented by two species Streptococcus thermophilus and Enterococcus feacalis. From a technological point of view, the camel milk can be used as a source of new starters in the development of the industrial manufacture of these strains.
Article 03 :Année de publication 2002
Titre Caractérisation des bactéries lactiques isolées du lait cru de chèvre des regions arides
   
Revue, référence J. Alg. Reg. Arides / Journal Algérien des Régions Arides, 1 : 01-11..
   
Autres auteurs Saidi N.
Guessas B.
Bensalah F.
Badis A.
Hadadji M.
Henni D.E.
Prevost H.
   
Mots Clefs Lait de chèvre, bactéries lactiques, identification, pouvoir acidifiant, Protéolyse
   
Résumé
   La diversité et la densité des bactéries lactiques du lait cru de chèvre des zones arides de l’ouest d’Algérie ont fait l'objet d’analyses phénotypiques, physiologiques et biochimiques. L'étude de 206 souches de bactéries lactiques a révélé la présence de 115 de formes cocci et 91 bâtonnets. Les cocci sont représentés par les genres Lactococcus (76,16%), Streptococcus thermophilus (14,78%) et Leuconostoc (8,6%). Lactococcus lactis subsp lactis est l’espèce dominante. Les espèces de lactobacilles détectées sont curvatus (25,25%), helviticus (10,98%), plantarum (9,89%), reuteri (9,89%), casei (7.69%), brevis (5,49%), bulgaricus (5,49%), paracasei (4,39%) et acidophilus (2,19%). Deux souches de Lactococcus lactis subsp lactis possèdent une activité protéolytique considérable. Le pouvoir acidifiant obtenu par les différentes souches testées en milieu lait se situe entre 42,2 mM et 110,6 mM en culture pure. En revanche, l’évaluation de la cinétique d’acidification en culture mixte fait apparaître différentes interactions positives et négatives.
Article 02 :Année de publication 2000
Titre تحديد المجامع المتكاملة خضريا عند عشيرة فطر Fusarium oxysporum f.sp. albedinis عزلت في منطقة بني عباس بالجهة الغربية من الجزائرٍ..
   
Revue, référence مجلة مؤتة للبحوث, مجلة مؤتة للبحوث و ألدراسات : مجلد 15 ، عدد 3.
   
Autres auteurs ج
م
ا
ل
ا
ل
د
ي
ن
ه
ـ
ـ
ن
ي
،
ا
ح
م
د
ب
ن
س
ل
ط
ا
ن
   
Mots Clefs التكامل الخضري. Fusarium oxysporum . الذبول الفيوزارى ,
   
Résumé
   درس التكامل الخضري عند عزلات لعشيرة فطر (Fusarium oxysporum ) تم الحصول عليها من نباتات طماطم مصابة بالذبول الفيوزارى ومن التربة المجاورة لها، و اورمات مخبرية مرجعية. الدراسة تمت عن طريق الاندماج الخلوى لكل العزلات الطافرة للانزيم المرجع للنترات. تم تحديد مجموعة متكاملة خضريا (VCG) تضم عزلات متجانسة ممرضة تنتمي كلها إلى (albedinis .Fusarium oxysporum f.sp ، اما العزلات الباقية فهي غير متكاملة ذاتيا و غير متكاملة مع الأفراد الاخرى مكونة بدلك مجامع خضرية بعدد العزلات المختبرة.
Article 01 :Année de publication 2000
Titre La flore lactique du lait cru de chamelle (Camelus dromedarius) d’Algérie.,
   
Revue, référence Camel newsletter, ASCAD. 17 : 64.
   
Autres auteurs Bensoltane A
Saidi Dj
   
Mots Clefs Camels’milk. Lactic Acid Bacteria. Proteins. Acidification,
   
Résumé
   The chemical composition of Algerian raw camels’ milk is slightly rich in fat 34.4 ± 2.8 g.l-1, proteins 33.1 ± 2.1 g.l-1, lactose 45.1 ± 3.1 g.l-1, ash 8.15 ± 0.15 g.l-1 and total solids 122.6± 0.12 g.l-1. Our results showed that total casein proteins were higher than whey proteins.Analytical electrophoresis showed that the whey proteins of camels’ milk were presented by 5 bands. The pH of raw milk decrease from 6.5 to 5.4 after 72 h of incubation at 30°C. The total count of lactic acid bacteria can reach 200 x 106 cfu ml-1 in MRS medium. Fourty strains of lactic acid bacteria were isolated from camel milk. The mesophilic group were represented by Lactococcus sp. Lactococcus lactis subsp. lactis biovar. diacetylactis which is the dominant strain. It produce more than 100 mM of lactic acid after 24 h of incubation at 30°C. the thermophilic strains were represented by two species Streptococcusthermophilus and Enterococcus feacalis. From a technological point of view, the camel milk can be used as a source of new starters in the development of the industrial manufacture of these strains.



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